“…In the past few years, the use of wavelength modulated absorption spectroscopy (WMAS) technology to monitor the growth of microorganisms has aroused considerable interest because of its characteristics including high sensitivity, high selectivity, high resolution, simple structure, and non‐invasiveness, which are indispensable to develop a fast online detection method 17–21 . Actually, it has been widely used in trace gas analysis, 22–24 chemical reaction process measurement, atmospheric environment monitoring, industrial combustion control, early disease diagnosis and screening, aeroengine stability test and many other application fields 25–28 . Several research groups have studied the utility of WMAS as a variety of fermentation process analysis techniques, such as Karla Martinez‐Cruz et al used WMS technology to estimate the capability of anaerobic biomass to treat effluents, 29 David Brueckner et al used WMS technology to evaluated on its potential to detect bacterial growth of contaminated media fill vials, 30 Anna Solokhina et al used WMS technology to measure the effect of isoniazid on metabolic activity and respiratory capability of mature Mycobacterium tuberculosis H37Ra (an avirulent strain) and Mycobacterium smegmatis biofilms 31 .…”