Performance efficiency of some biocontrol agents on controlling Cercospora leaf spot disease of sugar beet plants under organic agriculture system

Ahmed, Mohamed; Mikhail, Sherin P. H.; Shaheen, Samir I.

doi:10.1007/s10658-023-02729-5

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…Furthermore, several isolates are now capable of exhibiting multiple types of resistance [17]. To address this issue, biological control using different types of microorganisms (fungi, bacteria, and viruses) has emerged as a highly promising technology [18].…”

Section: Introductionmentioning

confidence: 99%

Evaluating Rhizobacterial Antagonists for Controlling Cercospora beticola and Promoting Growth in Beta vulgaris

El Housni,

Ezrari,

Radouane

et al. 2024

Microorganisms

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Cercospora beticola Sacc. is an ascomycete pathogen that causes Cercospora leaf spot in sugar beets (Beta vulgaris L.) and other related crops. It can lead to significant yield losses if not effectively managed. This study aimed to assess rhizosphere bacteria from sugar beet soil as a biological control agent against C. beticola and evaluate their effect on B. vulgaris. Following a dual-culture screening, 18 bacteria exhibiting over 50% inhibition were selected, with 6 of them demonstrating more than 80% control. The bacteria were identified by sequencing the 16S rRNA gene, revealing 12 potential species belonging to 6 genera, including Bacillus, which was represented by 4 species. Additionally, the biochemical and molecular properties of the bacteria were characterized in depth, as well as plant growth promotion. PCR analysis of the genes responsible for producing antifungal metabolites revealed that 83%, 78%, 89%, and 56% of the selected bacteria possessed bacillomycin-, iturin-, fengycin-, and surfactin-encoding genes, respectively. Infrared spectroscopy analysis confirmed the presence of a lipopeptide structure in the bacterial supernatant filtrate. Subsequently, the bacteria were assessed for their effect on sugar beet plants in controlled conditions. The bacteria exhibited notable capabilities, promoting growth in both roots and shoots, resulting in significant increases in root length and weight and shoot length. A field experiment with four bacterial candidates demonstrated good performance against C. beticola compared to the difenoconazole fungicide. These bacteria played a significant role in disease control, achieving a maximum efficacy of 77.42%, slightly below the 88.51% efficacy attained with difenoconazole. Additional field trials are necessary to verify the protective and growth-promoting effects of these candidates, whether applied individually, combined in consortia, or integrated with chemical inputs in sugar beet crop production.

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Section: Introductionmentioning

confidence: 99%

Evaluating Rhizobacterial Antagonists for Controlling Cercospora beticola and Promoting Growth in Beta vulgaris

El Housni,

Ezrari,

Radouane

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Furthermore, several isolates are now capable of exhibiting multiple types of resistance [17]. To address this issue, biological control using different types of microorganisms (Fungi, Bacteria, and Virus) emerges as highly promising technology [18].…”

Section: Introductionmentioning

confidence: 99%

Identifying Antagonistic Rhizobacterial Strains with Biocontrol Potential against <em>Cercospora beticola </em>and Assessing Their Growth-Promoting Effects on <em>Beta vulgaris</em>

El Housni,

Ezrari,

Radouane

et al. 2024

Preprint

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Cercospora beticola Sacc. is an ascomycete pathogen that causes Cercospora leaf spot in sugar beets (Beta vulgaris L.) and other related crops. The disease symptoms are characterized by circular lesions with an ash-gray centre and dark brown to purple margins on the leaves. It can lead to significant yield losses if not effectively managed. This study aims to characterize and assess the impact of bacterial biological control agents against C. beticola. Following a dual-culture screening, 18 bacteria exhibiting over 50% inhibition were selected, with six of them demonstrating more than 80% control. Identification of the bacteria was carried out through sequencing of the16S rDNA, revealing potentially 12 species belonging to six genera including Bacillus which was represented by four species. Additionally, the bacteria underwent in-depth characterization of biochemical and molecular properties, as well as plant-growth promotion. PCR detection of genes responsible for producing antifungal metabolites revealed that 83%, 78%, 89%, and 56% of the selected bacteria possessed the bacillomycin, iturin, fengycin, and surfactin encoding genes, respectively. Infrared spectroscopy analysis confirmed the presence of lipopeptide structure in the bacteria supernatant filtrate. Subsequently, the bacteria were assessed for their effect on sugar beet plants in controlled conditions. The bacteria exhibited notable capabilities, promoting growth in both roots and shoots, resulting in significant increases in root length, weight, and shoot length. The field experiment with four bacterial candidates demonstrated good performance against C. beticola compared to difenoconazole fungicide. These bacteria played a significant role in disease control, achieving a maximum efficacy of 77.42%, slightly below the 88.51% efficacy attained by difenoconazole. Additional field trials are necessary to verify the protective and growth-promoting effects of these candidates, whether applied individually, combined in consortia, or integrated with chemical inputs in sugar beet crop production.

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“…These issues have been the subject of a number of scientific studies [41][42][43]. In addition, there has been interest in organic fertilization [44][45][46][47][48], organic seed dressing [49], and biological control methods for sugar beet agrophages [50].…”

Section: Introductionmentioning

confidence: 99%

Optimizing an Organic Method of Sugar Beet Cultivation and Yield Gap Decrease in Northern Poland

Tyburski,

Nowakowski,

Nelke

et al. 2024

Agriculture

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In the period of 2016–2018, two series of field studies on organic sugar beet growing (Beta vulgaris L.) were carried out in northern Poland on Luvisol loamy soil (medium–heavy) soil in Bałcyny and Płonne. The aim of this study was to decrease the yield gap between organic and conventional beets. Factors to increase the yield of organic beet were differentiated fertilization (cattle farmyard manure (FYM), compost, and Bioilsa) and choice of varieties (Eliska, Jampol, and Sobieski). The reference point was the conventional cultivation of the same sugar beet varieties, fertilized with manure and NPK mineral fertilizers, the prevailing standard of sugar beet cultivation in Poland. High sugar beet root yields exceeding the average yield in Poland by 25–30% were obtained in both studies, both in conventional and organic cultivation. Higher root and white sugar yields were obtained in the study conducted at Płonne (with similar soil conditions to those at Bałcyny), but they were characterized by higher temperatures during the growing season. The lowest root yields in both experiments were obtained by fertilizing the organic beet with compost (66.1 t per ha in Bałcyny and 78.13 t per ha in Płonne), which were 10.8% and 8.5% lower than the conventional crop, respectively. Higher root yields in organic cultivation were obtained by fertilizing the sugar beet with FYM, which reduced the differences from conventional beet to 7.7% in the study in Bałcyny and 2.1% in the study in Płonne. Thus, the results showed no need to convert cattle FYM to compost. The highest root yields in organic cultivation were obtained by fertilizing the sugar beet with Bioilsa N 12.5 supplemented with mineral fertilization of K, Mg, and S (Patentkali). This fertilization provided a yield of 78.1 t of roots per ha in Bałcyny, which is a reduction in the yield gap to 1.4%, a statistically insignificant value. Moreover, in the study at Płonne, organic sugar beet fertilized with Bioilsa and Patentkali yielded 86.7 t of roots per ha, compared to 85.6 t per ha of conventional beet, so a yield gap was not seen here. The choice of varieties was also of great importance for root and pure sugar yields in both farming systems. The lowest yields were obtained from the Eliska variety, and at Bałcyny, a change of beet cultivar to Jampol increased the organic root yield from 68.8 t per ha to 76.0 t per ha, while reducing the yield gap from 10.1% to 2.2%. At Płonne, replacing the Eliska variety with Jampol reduced the yield gap between organic and conventional roots from 6.6% to 0.3%.

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Performance efficiency of some biocontrol agents on controlling Cercospora leaf spot disease of sugar beet plants under organic agriculture system

Cited by 3 publications

References 29 publications

Evaluating Rhizobacterial Antagonists for Controlling Cercospora beticola and Promoting Growth in Beta vulgaris

Evaluating Rhizobacterial Antagonists for Controlling Cercospora beticola and Promoting Growth in Beta vulgaris

Identifying Antagonistic Rhizobacterial Strains with Biocontrol Potential against <em>Cercospora beticola </em>and Assessing Their Growth-Promoting Effects on <em>Beta vulgaris</em>

Optimizing an Organic Method of Sugar Beet Cultivation and Yield Gap Decrease in Northern Poland

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