Performance characterization of continuous-wave laser-induced forward transfer of liquid bioink

Huang, Chiung Fang; Colley, Mamadi M.S.; Long, Lu; Chang, Chia Yu; Peng, Pai; Yang, Tzu Sen

doi:10.7567/1882-0786/ab4caf

Cited by 8 publications

(5 citation statements)

References 22 publications

(23 reference statements)

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“…The potential biomedical context of this study is alveolar bone to Ti64 dental implant. We are currently using the proposed a continuous-wave laser-induced forward transfer (CW-LIFT) system [37] for cell printing on the untreated and heat-treated Ti64 specimens for tissue engineering applications, and these results will be reported in the near future.…”

Section: Discussionmentioning

confidence: 99%

Effects of Heat Treatment of Selective Laser Melting Printed Ti-6Al-4V Specimens on Surface Texture Parameters and Cell Attachment

et al. 2021

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Selective laser melting (SLM) is extensively used for fabricating metallic biomedical products. After 3D printing, it is almost always advisable to apply a heat treatment to release the internal tensions or optimize the mechanical properties of the printed parts. The aim of this paper is to investigate the effects of heat treatment of SLM printed Ti-6Al-4V (Ti64) circular specimens on the areal surface texture parameters and cell attachment. Areal surface texture parameters, including the arithmetic mean height (Sa), root-mean-square height (Sq), skewness (Ssk), and kurtosis (Sku) were characterized. In addition, wavelet-based multi-resolution analysis was applied to investigate the characteristic length scales of untreated and heat-treated Ti64 specimens. In this study, the vertical distance between the highest and lowest position of cell attachment for each sampling area was defined as DH. Results showed that an increase in the periodic characteristic length scale was primarily due to the formation of large-scale aggregations of Ti64 metal powder particles on the heat-treated surface. In addition, MG-63 cells preferred lying in concave hollows; in heat-treated specimens, values of DH statistically significantly decreased from 31.6 ± 4.2 to 8.8 ± 2.8 μm, while Sku decreased from 3.3 ± 1.4 to 2.6 ± 0.6, indicating a strong influence of Sku on cell attachment.

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Section: Discussionmentioning

confidence: 99%

Effects of Heat Treatment of Selective Laser Melting Printed Ti-6Al-4V Specimens on Surface Texture Parameters and Cell Attachment

et al. 2021

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“…The spraying ejection dynamic observed for CW lasers when scanning the laser beam on the donor layer would not seem adequate for printing single pixels [26]. However, isolated pixels of bioinks have been successfully printed in a recent study by using an absorbing layer and controlling the power and exposure time [68]. In view of the long exposure times required, the printing speed of isolated pixels is hindered.…”

Section: Droplet Printingmentioning

confidence: 99%

Laser-Induced Forward Transfer: A Method for Printing Functional Inks

Fernández-Pradas

Serra

2020

Crystals

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Laser-induced forward transfer (LIFT) is a direct-writing technique based in the action of a laser to print a small fraction of material from a thin donor layer onto a receiving substrate. Solid donor films have been used since its origins, but the same principle of operation works for ink liquid films, too. LIFT is a nozzle-free printing technique that has almost no restrictions in the particle size and the viscosity of the ink to be printed. Thus, LIFT is a versatile technique capable for printing any functional material with which an ink can be formulated. Although its principle of operation is valid for solid and liquid layers, in this review we put the focus in the LIFT works performed with inks or liquid suspensions. The main elements of a LIFT experimental setup are described before explaining the mechanisms of ink ejection. Then, the printing outcomes are related with the ejection mechanisms and the parameters that control their characteristics. Finally, the main achievements of the technique for printing biomolecules, cells, and materials for printed electronic applications are presented.

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“…Thus, administering LLLT in the red to NIR range using a diode laser for PBM represents an innovative and non-invasive therapeutic approach for treating retinal injury and disease. We recently constructed a continuous-wave laser-induced forward transfer (CW-LIFT) system capable of controlling and precise depositing cells [14]. In addition, a single-cell NIR laser irradiation system (830 nm) and the image-based approaches were proposed for studying the modulatory effects in mitochondrial membrane potential (∆Ψm), reactive oxygen species (ROS), and vesicle transport in single living human adipose mesenchymal stem cells (hAD-SCs).…”

Section: Introductionmentioning

confidence: 99%

Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing

et al. 2022

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Our objective was to assess the effect of low-level laser therapy (LLLT) administered using a diode laser on the growth processes of human fibroblast cells involved in wound healing. Initially, studies were conducted using a diode laser at wavelengths of 633, 520, and 450 nm with an irradiance of 3 mW/cm2. The distance between the light source and culture plate was 3 cm. The mechanism(s) of action of the diode laser illumination on human fibroblast cells were studied by examining different wavelengths to determine the relevant light parameters for optimal treatment. In addition, the percentages of fibroblast-mediated procollagen and matrix metallopeptidase (MMP)-1, -2, and -9 production were compared. In the clinical study, the changes in basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and fibroblast collagen production were assessed in 60 patients with complicated wounds who received LLLT (633 nm). No statistically significant difference was observed between red light versus green and blue light in the viability analysis. In addition, the effects of LLLT on the cell cultures of fibroblast cells in vitro demonstrated a decrease in the relative expression of MMP-1, -2, and -9 while using light with a wavelength of 633 nm. In the clinical study, 633 nm diode laser LLLT at 2–8 J/cm2 was administered to 60 patients with complicated wounds; all patients showed increased levels of bFGF and VEGF and the occurrence of collagen synthesis. Our studies demonstrated that LLLT might affect fibroblast cell growth processes involved in wound healing.

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Performance characterization of continuous-wave laser-induced forward transfer of liquid bioink

Cited by 8 publications

References 22 publications

Effects of Heat Treatment of Selective Laser Melting Printed Ti-6Al-4V Specimens on Surface Texture Parameters and Cell Attachment

Effects of Heat Treatment of Selective Laser Melting Printed Ti-6Al-4V Specimens on Surface Texture Parameters and Cell Attachment

Laser-Induced Forward Transfer: A Method for Printing Functional Inks

Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing

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