Performance and Practicability Evaluation of five Cardiac Troponin I Assays in Patients With Acute Coronary Syndrom

Saadeddin, Salam M.; Habbab, Moh’d A.; Siddieg, Hisham; Seeni, Mohammed Al; Tahery, Ashraf B.; Dafterdar, Rofaida

doi:10.1378/chest.124.4_meetingabstracts.153s-a

Cited by 6 publications

(8 citation statements)

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“…The aims of the present study were to determine whether high-intensity exercise elevates cTnI concentrations in fit, athletic, clinically normal horses and to determine at approximately what time after exercise cTnI concentrations peak, in order to determine when to sample horses with suspected exercise-induced cardiac disease. This results in values that are not directly comparable from one analyser to another 12,[47][48][49][50][51][52] . The ranges we obtained in this group of horses at rest were similar to that of concentrations our lab has determined to be normal (# 0.08 ng ml 21 ) using the Stratus CS (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Acute effects of short duration, maximal exercise on cardiac troponin I in healthy horses

Durando

Reef

Kline

et al. 2006

Equine Comp. Exerc. Physiol.

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This study evaluated the effects of exercise on cardiac troponin I (cTnI) concentrations in healthy, adult horses. Fifteen fit, healthy horses determined to have a normal cardiovascular system completed a standardized exercise test on a high-speed treadmill. Heparinized blood was collected for plasma cTnI concentrations before maximal exercise, and 1, 3, 6, 9, 12 and 24 h post-exercise. The cTnI concentrations were measured with a commercial system (Stratus CS, Dade Behring, Inc.). Results were analysed by a multivariate ANOVA, where indicated post hoc analysis was done by Tukey-Kramer HSD and significance was placed at p , 0.05.All horses had elevations in cTnI concentrations after maximal exercise. Values for cTnI trended higher at 3 h (0.066^0.011 ng ml 21 ) and 6 h (0.062^0.011 ng ml 21 ) post-exercise compared with pre-exercise (0.039^0.007 ng ml 21 ), although this did not reach statistical significance. Mean cTnI concentrations were within our normal reference range at all time points, although four individuals were above our normal range after exercise.These data show that short-term, high-intensity exercise induces a small rise in plasma cTnI in normal horses. This should be kept in mind when evaluating cTnI concentrations in horses that have recently completed intense exercise. In addition, these data suggest that 3-6 h after intense exercise may be the optimal time for measurement of cTnI concentrations in horses with suspected exercise-induced myocardial damage.

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Section: Discussionmentioning

confidence: 99%

Acute effects of short duration, maximal exercise on cardiac troponin I in healthy horses

Durando

Reef

Kline

et al. 2006

Equine Comp. Exerc. Physiol.

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“…[7][8][9][10][11][12] Cardiac troponin I analyzers have not been standardized and because each company can use a potentially different and proprietary target amino acid in the assay, values obtained on 1 analyzer may not be directly comparable to values obtained on a different analyzer. [13][14][15][16] In addition, assays may have different reference ranges because of assay differences in the antibody specificity for free and complexed cTnI. The predominant circulating form of cardiac troponins in human beings is cTnI complexed to cardiac troponin C; however, the predominant circulating form in dogs is not known.…”

mentioning

confidence: 99%

“…[17][18][19] Several studies in human medicine have compared analyzers, and although results obtained on different analyzers correlate well, a large (up to 20-fold) difference in the absolute values obtained between some machines has been observed. [13][14][15]17,20,21 The use of and the potential applications for cTnI analysis are growing in veterinary medicine; however, the inability to compare values obtained on different analyzers has limited collaborative studies between institutions to this point. Reference ranges for dogs have been developed for several different analyzers using the 95th percentile as the upper end of the range, and the established ranges are similar for 3 of these analyzers (Biosite Triage Meter a ,0.05-0.12 ng/mL, Dade-Behring Stratus b ,0.03-0.07 ng/mL, and Beckman-Coulter Access AccuTnI c 0.01-0.11 ng/mL).…”

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confidence: 99%

Comparison of Canine Cardiac Troponin I Concentrations as Determined by 3 Analyzers

Adin

Oyama

Sleeper

et al. 2006

Veterinary Internal Medicne

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Background: Recent interest in cardiac biomarkers has led to the validation of several commercial analyzers for cardiac troponin I (cTnI) evaluation in dogs; however, these analyzers have not been standardized.Hypothesis: It was hypothesized that canine plasma cTnI concentrations as determined by 3 different analyzers would be similar.Animals: Twenty-three dogs with cardiac disease were studied. Methods: Reconstituted purified canine free cTnI was diluted with canine plasma to 8 concentrations (0.01, 0.1, 0.78, 1.56, 3.13, 6.25, 12.5, and 25 ng/mL), for analysis by 3 analyzers, the Biosite Triage Meter, the Dade-Behring Stratus, and the Beckman-Coulter Access AccuTnI. Plasma samples from 23 dogs with cardiac disease were also analyzed for cTnI concentrations on all analyzers.Results: Troponin I concentrations in sick dogs were ,0.05-5.72 ng/mL (Biosite), 0.02-11.1 ng/mL (Access), and 0.02-9.73 ng/mL (Stratus). Analyzer results were highly correlated with each other (r 5 0.97 to 1.0 for purified dilutions, r 5 0.61 to 0.89 for samples from dogs); however, the limits of agreement were wide for both purified dilutions and clinical samples. Recovery was highest for the Access (334-1467%) and lowest for the Biosite (38-60%); Stratus 52-233%. Analyzer variability was lowest for the Access (1.2-10.4%) and highest for the Stratus (4.8-33.6%); Biosite 2.8-16.5%.Conclusions and Clinical Importance: Results from this study suggest that although canine cTnI values obtained from the Biosite, Stratus, and Access analyzers are closely correlated, they cannot be directly compared with each other. In the absence of a gold standard none of the analyzers can be considered more correct than the others.

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“…Degradation products of TnT were also found both in animal models of ischemia/reperfusion injury [38,39] and in serum of patients with AMI [40,41]. In 2000, TnI and TnT have been approved as the biomarkers for AMI diagnosis [42], and several kits have been commercially developed and used in clinical studies [43][44][45]. In addition to TnI and TnT, other myofilament proteins, including TnC [46], LC2 [46], aactinin [32] and MyBP-C [47], were also reported as useful markers in animal models of ischemia/reperfusion injury.…”

Section: Degradationmentioning

confidence: 99%

“…Alternatively, the degradation site can be mapped using antibody epitope analysis [62], in which an array of mAb recognizing different regions within protein sequences is used [43][44][45].…”

Section: Degradationmentioning

confidence: 99%

Myofilament proteins: From cardiac disorders to proteomic changes

Yuan

Solaro

2008

Proteomics Clinical Apps

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Myofilament proteins of the cardiac sarcomere house the molecular machinery responsible for generating tension and pressure. Release of intracellular Ca 21 triggers myofilament tension generation and shortening, but the response to Ca 21 is modulated by changes in key regulatory proteins. We review how these proteomic changes are essential to adaptive physiological regulation of cardiac output and become maladaptive in cardiac disorders. We also review the essentials of proteomic techniques used to study myofilament protein changes, including degradation, isoform expression, phosphorylation and oxidation. Selected proteomic studies illustrate the applications of these approaches.

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Performance and Practicability Evaluation of five Cardiac Troponin I Assays in Patients With Acute Coronary Syndrom

Cited by 6 publications

References 0 publications

Acute effects of short duration, maximal exercise on cardiac troponin I in healthy horses

Acute effects of short duration, maximal exercise on cardiac troponin I in healthy horses

Comparison of Canine Cardiac Troponin I Concentrations as Determined by 3 Analyzers

Myofilament proteins: From cardiac disorders to proteomic changes

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