Performance and potential clinical impact of Alfred60AST (Alifax®) for direct antimicrobial susceptibility testing on positive blood culture bottles

Abstract

Performance and potential clinical impact of Alfred60(AST) (Alifax®) for direct antimicrobial susceptibility testing on positive blood culture bottles

Antimicrobial susceptibility testing determined by Alfred 60/AST (Alifax®) in a multi-sites lab: performance's evaluation and optimization of workflow

“…The analytical performances of enterococci were very good and For Enterobacterales, we highlighted very good results for amoxicillinclavulanate and better results for cefotaxime (CA at 97.8% versus between 89.7 and 97.8%) and piperacillin-tazobactam (CA at 93.3% versus between 77.3 and 85.7%) AST than in other studies (Barnini et al, 2016;Giordano et al, 2018;Van den Poel et al, 2020). Indeed, piperacillintazobactam antibiotic reagent has been reviewed by Alifax® and marketed in October 2018 because of previous high rates of VME and ME.…”

“…showed a CA of 100% for both ampicillin and vancomycin. For staphylococci, Alfred 60/AST cannot detect inducible clindamycin resistance as we obtained a high rate of VME (40.9%) such as those of other studies (12% to 29.4%)(Anton-Vazquez et al, 2019;Van den Poel et al, 2020).…”

“…For meropenem, 9 ME were observed with a rate of 6.2% which was close to that described by Giordano & all. It should be pointed out that meropenem ME rate (1.2% to 14.3%) is variable between studies ( Barnini et al, 2016;Giordano et al, 2018;Van den Poel et al, 2020). We hypothesize that this variation could be due to an unstable antibiotic reagent.…”

Rapid Detection of Imipenem Resistance in Gram-Negative Bacteria Using Tabletop Scanning Electron Microscopy: A Preliminary Evaluation

“…This method allowed to improve the AST of Gram-negative bacilli toward IPM, where the whole process from sample preparation to results yield takes 1 to 2 h depending on the specie, whereas for the routinely used techniques, results can take up to 48 h before judgment. Novel technologies available on the market also reduced the AST time-to-result, such as Quantamatrix direct rapid AST yielding results after 6 h (Wang et al, 2017 ), Accelerate Pheno-Systems generating AST results within 7 h (Charnot-Katsikas et al, 2018 ; De Angelis et al, 2019 ), and Alifax Alfred60 AST with a time-to-result of 5 h (Boland et al, 2019 ; Van den Poel et al, 2020 ). However, costs are very high to be applied globally, especially in under-developed countries.…”

Evaluation of the Performance of Filmarray Blood Culture Identification Panel on Detecting Blood Cultures Containing Activated Carbon Powder

“…So far, blood culture (BC) is an essential tool for BSI diagnosis, but its time-consuming turn-around-time obstructs the timely treatment of patients. Several new technologies such as MALDI-TOF mass spectrometer have been adapted by microbiology laboratories to shorten the time spend on microbial identification [3, 4], but it relies on the outcome of the subculture on the agar plate, which may take several additional days after the blood culture.…”