Performance and microbial characteristics of integrated fixed-film activated sludge system treating industrial wastewater

Li, C.; Li, X. L.; Ji, Min; Liu, J.

doi:10.2166/wst.2012.421

Cited by 23 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparable research in this area generally reports a trend of greater AOB abundance and/or nitrification activity in IFAS media relative to the ML fraction [33,34,45]; although this trend is apparently not universal (e.g., Li et al [32]). The above correlations, in particular the positive association between media biofilm AOB amoA abundance and %NH 4 + -N removal, complement the general findings of prior IFAS studies.…”

Section: Resultsmentioning

confidence: 99%

Application of a Novel Functional Gene Microarray to Probe the Functional Ecology of Ammonia Oxidation in Nitrifying Activated Sludge

et al. 2013

View full text Add to dashboard Cite

We report on the first study trialling a newly-developed, functional gene microarray (FGA) for characterising bacterial and archaeal ammonia oxidisers in activated sludge. Mixed liquor (ML) and media biofilm samples from a full-scale integrated fixed-film activated sludge (IFAS) plant were analysed with the FGA to profile the diversity and relative abundance of ammonia-oxidising archaea and bacteria (AOA and AOB respectively). FGA analyses of AOA and AOB communities revealed ubiquitous distribution of AOA across all samples – an important finding for these newly-discovered and poorly characterised organisms. Results also revealed striking differences in the functional ecology of attached versus suspended communities within the IFAS reactor. Quantitative assessment of AOB and AOA functional gene abundance revealed a dominance of AOB in the ML and approximately equal distribution of AOA and AOB in the media-attached biofilm. Subsequent correlations of functional gene abundance data with key water quality parameters suggested an important functional role for media-attached AOB in particular for IFAS reactor nitrification performance and indicate possible functional redundancy in some IFAS ammonia oxidiser communities. Results from this investigation demonstrate the capacity of the FGA to resolve subtle ecological shifts in key microbial communities in nitrifying activated sludge and indicate its value as a tool for better understanding the linkages between the ecology and performance of these engineered systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Application of a Novel Functional Gene Microarray to Probe the Functional Ecology of Ammonia Oxidation in Nitrifying Activated Sludge

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The pilot IFAS system as well as the characteristics of the bio-carriers, wastewater and the seeded activated sludge used in the system had all been described in our previous research [8]. The system included an anaerobic, an anoxic and two aerobic reactors.…”

Section: A Description Of Ifas System and Samplingmentioning

confidence: 99%

“…Pretreatment and DNA extraction of sludge samples were conducted following the established method [8].…”

Section: B Dna Extraction and Nested Pcrmentioning

confidence: 99%

See 1 more Smart Citation

Community Structure and Quantification of Nitrifying Bacteria in Integrated Fixed Film Activated Sludge System Treating Industrial Wastewater

Li¹,

Xi²,

Bi³

2015

Proceedings of the 2nd International Conference on Green Materials and Environmental Engineering

View full text Add to dashboard Cite

Abstract-To reveal the community structure and abundance of ammonia-oxidizing bacterial (AOB) and nitrite-oxidizing bacterial (NOB) in integrated fixed film activated sludge process (IFAS) treating industrial wastewater, the molecular biological techniques of polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), cloning and sequencing and realtime quantitative PCR were applied. DGGE results showed that in the process of the experiment, the dominant species of suspended sludge and biofilm were different and that some kinds of populations had been highly preponderance consistently. Additionally, variation in influents had no significant effect on the distribution of the AOB and NOB communities. Sequencing analysis of screened clones revealed Nitrosospira sp. was the particular AOB population in suspended sludge, and some members of Nitrosomonas sp. are helpful to keep the biomass remained in the bio-carrier surface. Real-time PCR indicated that the proportion of the AOB and NOB in total amount of bacteria increased significantly after the domestication period. The AOB and NOB population in the suspended sludge were even higher than the content of the biofilm.

show abstract

“…The suspended-growth and biofilms communities from an integrated fixed film activated sludge (IFAS) process were analyzed using denaturing gradient gel electrophoresis (DGGE) and FISH. Denitrifying bacteria were identified in the biofilm and species associated with the genera Firmicutes and Pseudomonas were implicated in organic degradation Li et al (2012a).…”

mentioning

confidence: 99%

Novel Applications of Molecular Biological and Microscopic Tools in Environmental Engineering

Gedalanga¹,

Kotay²,

Sales³

et al. 2013

Water Environment Research

View full text Add to dashboard Cite

Molecular biological methods offer flexible and powerful tools to environmental practitioners and researchers interested in studying environmental challenges in natural and engineered systems. In recent years, these techniques have allowed investigators to connect the fate, transport, and transformation of environmental chemical contaminants and pathogens with biological processes of functionally diverse microorganisms or microbial communities. Indeed, the boundaries of microbial ecosystems are constantly refined as researchers discover new links that extend beyond Bacteria to include Archaea and unicellular Eukarya.  Quantitative polymerase chain reaction (qPCR) provides a rapid and sensitive approach to determine gene abundance and expression from a wide range of microorganisms from complex environments. Whole genome arrays (WGA) and functional gene arrays (FGA) are being used to elucidate transcriptional changes in response to environmental parameters. Antibiotic resistance profiling and microbial source tracking studies continue to benefit from the information provided by a molecular‐based experimental design. Quantitative fluorescent in situ hybridization (qFISH) and next generation sequencing technologies are changing the way we view suspended solids in wastewater treatment. Innovative sensors are being developed that couple molecular biological, chemical, or physical properties to improve the sensitivity and specificity for intended targets. Thus, advanced molecular analysis complements conventional approaches to provide a more comprehensive understanding of microbes and microbial processes in the environment. The purpose of this review is to summarize high impact studies and novel applications of molecular methods for environmental engineering professionals and scientists.

show abstract

Performance and microbial characteristics of integrated fixed-film activated sludge system treating industrial wastewater

Cited by 23 publications

References 0 publications

Application of a Novel Functional Gene Microarray to Probe the Functional Ecology of Ammonia Oxidation in Nitrifying Activated Sludge

Application of a Novel Functional Gene Microarray to Probe the Functional Ecology of Ammonia Oxidation in Nitrifying Activated Sludge

Community Structure and Quantification of Nitrifying Bacteria in Integrated Fixed Film Activated Sludge System Treating Industrial Wastewater

Novel Applications of Molecular Biological and Microscopic Tools in Environmental Engineering

Contact Info

Product

Resources

About