Performance and Limitations of Phosphate Quantification: Guidelines for Plant Biologists

Kanno, Satoshi; Cuyas, Laura; Javot, Hélène; Bligny, Richard; Gout, Elisabeth; Dartevelle, Thibault; Hanchi, Mohamed; Nakanishi, Teruyuki; Thibaud, Marie-Christine; Nussaume, Laurent

doi:10.1093/pcp/pcv208

Cited by 54 publications

(53 citation statements)

References 109 publications

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“…) and inorganic phosphate ( c . 50–70 μmol g −1 DW for plants grown in high‐phosphate conditions, calculations from data in Kanno et al ., ). The huge difference ( c .…”

Section: Discussionmentioning

confidence: 97%

Arabidopsis thaliana plants challenged with uranium reveal new insights into iron and phosphate homeostasis

et al. 2017

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Uranium (U) is a naturally occurring radionuclide that is toxic to plants. It is known to interfere with phosphate nutrition and to modify the expression of iron (Fe)-responsive genes. The transporters involved in the uptake of U from the environment are unknown. Here, we addressed whether IRT1, a high-affinity Fe transporter, could contribute to U uptake in Arabidopsis thaliana. An irt1 null mutant was grown hydroponically in different conditions of Fe bioavailability and phosphate supply, and challenged with uranyl. Several physiological parameters (fitness, photosynthesis) were measured to evaluate the response to U treatment. We found that IRT1 is not a major route for U uptake in our experimental conditions. However, the analysis of irt1 indicated that uranyl interferes with Fe and phosphate homeostasis at different levels. In phosphate-sufficient conditions, the absence of the cation chelator EDTA in the medium has drastic consequences on the physiology of irt1, with important symptoms of Fe deficiency in chloroplasts. These effects are counterbalanced by U, probably because the radionuclide competes with Fe for complexation with phosphate and thus releases active Fe for metabolic and biogenic processes. Our study reveals that challenging plants with U is useful to decipher the complex interplay between Fe and phosphate.

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“…) and inorganic phosphate ( c . 50–70 μmol g −1 DW for plants grown in high‐phosphate conditions, calculations from data in Kanno et al ., ). The huge difference ( c .…”

Section: Discussionmentioning

confidence: 97%

Arabidopsis thaliana plants challenged with uranium reveal new insights into iron and phosphate homeostasis

et al. 2017

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“…In (b), plants were grown for 10 days on MGRL agar media supplemented with 1500 µM (+S) or 15 µM sulfate (-S). Plants were then transferred to +S or -S hydroponic medium containing 32 Furthermore, we analyzed the Pi uptake using the radioactive isotope of phosphorus ( 32 P) ( [45,46], Figure 2b). Plants grown for 10 days on +S or -S agar media were transferred to +S or -S hydroponic solution containing 32 P. After 60 min, the intensity of 32 P were significantly higher in plants subjected to -S comparing to that subjected to +S (Figure 2b), indicating an enhancement of Pi uptake as an early response to -S.…”

Section: Of 15mentioning

confidence: 99%

Sulfur Deficiency Increases Phosphate Accumulation, Uptake, and Transport in Arabidopsis thaliana

Allahham

Kanno

Zhang

et al. 2020

IJMS

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Recent studies have shown various metabolic and transcriptomic interactions between sulfur (S) and phosphorus (P) in plants. However, most studies have focused on the effects of phosphate (Pi) availability and P signaling pathways on S homeostasis, whereas the effects of S availability on P homeostasis remain largely unknown. In this study, we investigated the interactions between S and P from the perspective of S availability. We investigated the effects of S availability on Pi uptake, transport, and accumulation in Arabidopsis thaliana grown under sulfur sufficiency (+S) and deficiency (−S). Total P in shoots was significantly increased under −S owing to higher Pi accumulation. This accumulation was facilitated by increased Pi uptake under −S. In addition, −S increased root-to-shoot Pi transport, which was indicated by the increased Pi levels in xylem sap under −S. The −S-increased Pi level in the xylem sap was diminished in the disruption lines of PHT1;9 and PHO1, which are involved in root-to-shoot Pi transport. Our findings indicate a new aspect of the interaction between S and P by listing the increased Pi accumulation as part of −S responses and by highlighting the effects of −S on Pi uptake, transport, and homeostasis.

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“…Total Pi in the samples was estimated from crude cell extracts using the molybdenum blue method as described (Kanno et al 2016). Total Pi levels in samples were normalized by the amount of protein in the samples.…”

Section: Estimation Of Intracellular Pimentioning

confidence: 99%

Protein synthesis controls phosphate homeostasis

Pontes

Groisman

2018

Genes Dev.

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Phosphorus is an essential element assimilated largely as orthophosphate (Pi). Cells respond to Pi starvation by importing Pi from their surroundings. We now report that impaired protein synthesis alone triggers a Pi starvation response even when Pi is plentiful in the extracellular milieu. In the bacterium serovar Typhimurium this response entails phosphorylation of the regulatory protein PhoB and transcription of PhoB-dependent Pi transporter genes and is eliminated upon stimulation of adenosine triphosphate (ATP) hydrolysis. When protein synthesis is impaired due to low cytoplasmic magnesium (Mg), triggers the Pi starvation response because ribosomes are destabilized, which reduces ATP consumption and thus free cytoplasmic Pi. This response is transient because low cytoplasmic Mg promotes an uptake in Mg and a decrease in ATP levels, which stabilizes ribosomes, resulting in ATP consumption and Pi increase, thus ending the response. Notably, pharmacological inhibition of protein synthesis also elicited a Pi starvation response in the bacterium and the yeast Our findings identify a regulatory connection between protein synthesis and Pi homeostasis that is widespread in nature.

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Performance and Limitations of Phosphate Quantification: Guidelines for Plant Biologists

Cited by 54 publications

References 109 publications

Arabidopsis thaliana plants challenged with uranium reveal new insights into iron and phosphate homeostasis

Arabidopsis thaliana plants challenged with uranium reveal new insights into iron and phosphate homeostasis

Sulfur Deficiency Increases Phosphate Accumulation, Uptake, and Transport in Arabidopsis thaliana

Protein synthesis controls phosphate homeostasis

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