Performance and immunological resistance of Litopenaeus vannamei fed a β-1,3/1,6-glucan-supplemented diet after per os challenge with the Infectious myonecrosis virus (IMNV)

Abstract

-This study determined the efficacy of a β-1,3/1,6-glucan source on the survival, growth, and immunological responses of Litopenaeus vannamei challenged with infectious myonecrosis virus (IMNV). The work was conducted in 20 tanks of 500 L with clear water with constant recirculation and aeration. Shrimp weighing 2.6±0.4 g were stocked at 100 animals/m 2 and reared for 10 weeks. Viral challenge occurred through oral administration of IMNV positive extract (1.82 £ × 103 copies of IMNV/µL RNA) during three consecutive days, twice a day. One treatment and three controls, with five replicate tanks each, were used, as follows: Com -commercial diet fed to non-challenged shrimp (

Effect of biofloc technology in Farfantepenaeus aztecus culture: The optimization of dietary protein level on growth performance, digestive enzyme activity, non‐specific immune response, and intestinal microbiota

“…Subsequently, the sample was centrifuged three times at 15000 g for 15 min. The resulting serum was collected, transferred to an Eppendorf tube, and stored at −20°C until analysis (Sabry Neto & Nunes, 2015).…”