Abstract-In order to reduce the dependency on fossil oil energy resources, the using of several energy resources in compression-ignition engine has been the target of attention of researchers. The major renewable alternative combustible species are methanol, biogas, ethanol, biodiesel, and hydrogen. Fusel oil is a by-product of bioethanol production during the fermentation process present one of the new alternative fuels for compression-ignition engine. The aim of this work is to experimentally investigate the effect of fusel oil -diesel blending on performance and emission in single cylinder compression ignition engines. The test achieved under 25 % engine load at five-engine speed 1200 to 2400 rpm with intervals of 300 rpm. The experimental results revealed that the engine indicted power and torque slightly decrease for F 2 0 compared with pure diesel, and the increment was happened in fuel consumption. Although diesel fuel yields, the highest volumetric efficiency and exhaust temperature. Moreover, the results showed CO 2 and CO were increased while the NOx emissions decrease with the use F 2 0. All the negative effects that happened in power and torque was because of the water content, low heating value, low cetane number for fusel oil.Index Terms-Fusel oil, NOx emissions, single cylinder engine, alternative fuels, engine performance.I.