Perfluorodecanoic acid and lipid metabolism in the rat

Abstract

Alterations in lipid metabolism were examined in adult male Sprague-Dawley rats seven days after a single intraperitoneal injection of perfluorodecanoic acid (PFDA; 20, 40 or 80 mg/kg). Because PFDA treatment caused a dose-related reduction in feed intake, the response of vehicle-treated rats pair-fed to those receiving PFDA was monitored to distinguish direct effects of the perfluorinated fatty acid from those secondary to hypophagia. Carcass content of lipid phosphorus and free cholesterol decreased in dose-dependent fashion in both PFDA-treated and pair-fed rats. Carcass triacylglycerols diminished in a similar manner, yet PFDA-treated rats at each dose had a higher concentration of neutral acylglycerols than their vehicle-treated, pair-fed counterparts. In vehicle-treated, pair-fed rats at the 80 mg/kg dose level, lipid phosphorus and free cholesterol as a proportion of carcass fat increased, whereas the share of the triacylglycerols declined. Because of the higher concentration of triacylglycerols in the carcass of rats treated with 80 mg/kg PFDA, enrichment of lipid phosphorus and free cholesterol in carcass fat was less than in their pair-fed partners. The amount of lipid phosphorus and free cholesterol per hepatocyte was similar in both PFDA-treated rats and their pair-fed partners. Liver triacylglycerols were markedly increased in PFDA-treated rats. A similar but less extensive augmentary effect of PFDA on hepatic esterified cholesterol was found. Concentration of triacylglycerols in plasma was not elevated in PFDA-treated rats, in spite of hepatic accumulation of esterified compounds. Also, the plasma level of free fatty acids and 3-hydroxybutyrate was similar in all treatment groups, including those receiving PFDA.(ABSTRACT TRUNCATED AT 250 WORDS)

Effect of the peroxisome proliferator perfluorodecanoic acid on growth and lipid metabolism in Sprague Dawley rats fed three dietary levels of selenium

“…In contrast, administration of other peroxisome proliferators to rats, such as clofibrate or fenofibrate, result in increases in both fatty acyl-CoA oxidase activity and peroxisomal 13-oxidation rate (Moody and Reddy 1978a, b;Cohen and Grasso 1981;Hawkins et al, 1987;Steinberg et al 1988). The PFDA-induced accumulation of liver fatty acids and triglycerides (George and Andersen 1986;Van Rafelghem et al 1987), substrates for fatty acyl-CoA oxidase, may lead to increases in this enzyme (Hawkins et al 1987). However, the increase in PFDA-induced fatty acyl-CoA oxidase activity was not accompanied by an increase in peroxisomal [3-oxidation.…”

“…Along with the peroxisomal proliferation, increases in peroxisomal fatty acyl-CoA oxidase activity have also been reported (Harrison et al 1988). These changes may contribute to the disruption of lipid metabolism in the liver (Van Rafelghem et al 1987).…”

Effects of 8-2 Fluorotelomer Alcohol on Oleic Acid Formation in the Liver of Rats

“…24,25) Therefore, these compounds may affect human or animal health through alterations in energy metabolism since oleic acid is a major fatty acid synthesized via de novo pathway and enriched in triglyceride. However, information is lacking about the effect of 8-2 fluorotelomer alcohol on fatty acid composition in the liver of animals.…”

Induction of Triglyceride Accumulation in the Liver of Rats by Perfluorinated Fatty Acids with Different Carbon Chain Lengths: Comparison with Induction of Peroxisomal .BETA.-Oxidation.

“…4) Perfluorodecanoic acid (C10), a PFCA with a 10 carbon chain length, has also been reported to cause considerable lipid accumulation in the liver of male rats, 7) and the potency of C10 in TG accumulation appears to be much greater than that of C8. 4,7) These results raise the question of whether the induction of hepatic TG accumulation is specific to PFCAs with longer carbon chain lengths. There is another major difference in the induction of enzymes such as peroxisomal b-oxidation between C8 and C10.…”

“…[2][3][4][5][6] In addition to the above-mentioned effects, C8 accumulates triglyceride (TG) in the liver of male rats. 4) Perfluorodecanoic acid (C10), a PFCA with a 10 carbon chain length, has also been reported to cause considerable lipid accumulation in the liver of male rats, 7) and the potency of C10 in TG accumulation appears to be much greater than that of C8. 4,7) These results raise the question of whether the induction of hepatic TG accumulation is specific to PFCAs with longer carbon chain lengths.…”