Perfluorodecalin-supported system enhances taxane production in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene

Sykłowska-Baranek, Katarzyna; Pilarek, Maciej; Bonfill, Mercedes; Kafel, K.; Pietrosiuk, Agnieszka

doi:10.1007/s11240-014-0659-1

Cited by 33 publications

(41 citation statements)

References 42 publications

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“…The results obtained in previous research performed by our group indicated that direct application of PFD to the culture system constitutes simple and efficient conditions for the enhancement of secondary metabolites production (Sykłowska-Baranek et al 2014, 2015a. In cultures of KT root line supplemented with PFD-aerated or degassed but without elicitors no lignan content was detected (Table 4).…”

Section: Lignan Accumulation In Cultures Of Atma and Kt Hairy Root LImentioning

confidence: 77%

“…2d-f). In the previous investigation of our group performed in ATMA root line cultures the diminished biomass growth was noted as a result of PFD-aerated or degassed applied alone or in combination with 100 µM MeJa with a marked tendency of more pronounced growth suppression in PFD-aerated supported cultures (Sykłowska-Baranek et al 2015a). …”

Section: Pfd-phases Supported Cultures Of Atma and Kt Hairy Root Linesmentioning

confidence: 87%

“…The first hairy root line-KT was established by transformation with wild type Agrobacterium rhizogenes LBA 9402 strain (Furmanowa and Syklowska-Baranek 2000). While the second hairy root line-ATMA was obtained as a result of direct inoculation of 10-year-old Taxus plantlets cultivated in vitro with the C58C1 strain of Agrobacterium tumefaciens carrying the plasmid RiA4 of A. rhizogenes and the binary plasmid pCAMBIA-TXS-His, harbouring the taxadiene synthase (txs) gene of T. baccata (GenBank accession: AY424738), under the control of the 35S CaMV promoter, and the hygromycin phosphotransferase gene (hptII) as a resistance marker (Sykłowska-Baranek et al 2015a). The cultures of KT and ATMA hairy root lines were established in 1996 and 2008, respectively.…”

Section: Hairy Root Culturesmentioning

confidence: 99%

“…PFD-aerated applied to the cell suspension cultures of Arnebia euchroma allowed to enhance naphthoquinone yield about 50% in comparison to control cultures (Sykłowska-Baranek et al 2014). The influence of PFD application at two time points: the day of inoculation (day 0) and the 14th day of culture together with elicitation (100 µM PHEN and MeJa) in ATMA transformed root cultures was studied (Sykłowska-Baranek et al 2015a). It was demonstrated that the most suitable time point for PFD addition is the 14th day of culture and the highest total (i.e.…”

Section: Lignan Accumulation In Cultures Of Atma and Kt Hairy Root LImentioning

confidence: 99%

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Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Sykłowska-Baranek

Łysik

Jeziorek

et al. 2018

Plant Cell Tiss Organ Cult

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The biosynthetic potential for six lignans accumulation in two lines of Taxus x media hairy roots was investigated. The cultures of KT and ATMA hairy root lines were supplemented with precursors: coniferyl alcohol (CA 1, 10 or 100 µM) and/or l-phenylalanine (100 µM PHEN) and/or methyl jasmonate (100 µM MeJa). Moreover the two-phase in vitro cultures supported with perfluorodecalin (PFD) as a gas carrier and in situ extrahent were used. The hairy root lines differed in lignan production profiles. In the control untreated cultures KT roots did not accumulate secoisolariciresinol and lariciresinol while ATMA roots did not accumulate matairesinol. In ATMA roots the treatment with CA (1 or 10 µM) resulted in the production of lariciresinol and secoisolariciresinol whereas solely lariciresinol was present after 100 µM CA application. Elicitation with 1 µM CA and MeJa yielded with hydroxymatairesinol aglyca and lariciresinol glucosides with their highest content 37.88 and 3.19 µg/g DW, respectively. The stimulatory effect of simultaneous treatment with 1 µM CA, PHEN and MeJa on lignan production was observed when the cultures were supplemented with PFD-aerated or degassed. In ATMA root cultures these applied conditions were the most favourable for matairesinol content which amounted to 199.86 and 160.25 µg/g DW in PFD-aerated and PFD-degassed supported cultures, respectively. In KT root cultures solely, hydroxymatairesinol and coniferin/CA content was enhanced with their highest yield 59.29 and 134.60 µg/g DW in PFD-aerated and PFD-degassed cultures, respectively.

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Section: Lignan Accumulation In Cultures Of Atma and Kt Hairy Root LImentioning

confidence: 77%

Section: Pfd-phases Supported Cultures Of Atma and Kt Hairy Root Linesmentioning

confidence: 87%

Section: Hairy Root Culturesmentioning

confidence: 99%

Section: Lignan Accumulation In Cultures Of Atma and Kt Hairy Root LImentioning

confidence: 99%

See 2 more Smart Citations

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Sykłowska-Baranek

Łysik

Jeziorek

et al. 2018

Plant Cell Tiss Organ Cult

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“…Moreover, the txs synthase transgene has recently been successfully introduced in Taxus x media var. Hicksii, resulting in taxaneproducing transgenic hairy root cultures (Sykłowska-Baranek et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Paclitaxel production and PAL activity in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene elicited with nitric oxide and methyl jasmonate

et al. 2015

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The role of sodium nitroprusside (SNP, 10 lM), a nitric oxide (NO) donor, and/or methyl jasmonate (MJ, 100 lM) spiked with L-phenylalanine (PHEN, 100 lM) and additional sucrose (S; 30 g l -1 ), in taxane production and phenyl ammonia lyase (PAL) activity in cultures of two Taxus media x var. Hicksii transgenic root lines (ATMA and ATM) carrying the taxadiene synthase transgene was investigated. SNP addition, when applied together with MJ and/or PHEN, resulted in paclitaxel production only in ATMA cultures. The application of the NO donor gave the highest paclitaxel content (7.56 mg l -1) in the combination of SNP?S?MJ?PHEN, after 2 weeks of treatment in the ATMA root line. In ATM cultures, taxane production was not affected by SNP. In both ATMA and ATM lines the highest total (intra?extracellular) paclitaxel yield was determined when elicited with MJ?PHEN, and amounted to 10.78 mg l -1 at 1 week and 1.63 mg l -1 at 2 weeks of treatment, in cultures of ATMA and ATM lines, respectively. The excretion of paclitaxel was observed only in ATMA cultures, with the highest level (2.34 mg l -1 ) obtained after elicitation with S?MJ?PHEN. The comparison of PAL activity in the two root lines revealed that this enzyme was almost 3-times more active in ATM than ATMA roots. An increase in both PAL activity and paclitaxel production was only observed in ATMA cultures growing in medium supplemented with S?MJ?PHEN.

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