Perfil De Resistência Antimicrobiana De Isolados Bacterianos De Afecções Clínicas Do Hospital Veterinário Da Universidade Estadual De Maringá

Sfaciotte, Ricardo Antonio Pilegi; Vignoto, Vanessa Kelly Capóia; Wosiacki, Sheila Rezler

doi:10.4025/revcivet.v1i1.23281

Cited by 5 publications

(11 citation statements)

References 4 publications

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“…With regard to the multidrug resistance index, Arias et al [3] studied samples of infected surgical wounds, and found 19 out of 23 samples (82.6%) to have a MAR index ≥ 0.2, three of which with a value of 1. Sfaciotte et al [27] isolated 89.4% of the analyzed samples with a MAR index ≥ 0.2. Both studies thus registered a high index medium, however only a few samples were studied and only a few antimicrobial drugs were tested.…”

Section: Discussionmentioning

confidence: 99%

“…For isolated MRS detected in this study, chloramphenicol was the antimicrobial that showed the highest sensitivity, 74.29% (27/36); thus it is the most suitable antimicrobial for treatment of this type of micro-organism. In the PABA class of inhibitors, Sfaciotte et al [27] found resistance in 100% of the samples studied, Arias et al [3] concluded 92.3% (12/13) in samples of contaminated and infected wounds, and according to Dal-Bo et al [12] 75% to Staphylococcus sp. and 50% to Gram-negative bacilli.…”

Section: Discussionmentioning

confidence: 99%

“…The results were submitted for descriptive analysis in order to calculate absolute and relative frequencies [27].…”

Section: Methodsmentioning

confidence: 99%

“…The resistance rates towards antimicrobials in companion animals have risen considerably. Studies of local antimicrobial susceptibility profiles are needed as well as proper education and warning about the use of tests for the identification and susceptibility of pathogenic bacterial strains [18,27]. Thus, the aim of this study was to identify the main antimicrobial resistance in clinical samples of dogs and to detect the multidrug-resistant bacterial pathogens involved.…”

Section: Introducionmentioning

confidence: 99%

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Antimicrobial Resistance Phenotypic Profile of Isolates from Clinical Infections in Dogs

Sfaciotte

Coronel

Snak

et al. 2017

Acta Scientiae. Vet.

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Background: Antimicrobial resistance is described as a condition in which a micro-organism is able to survive when exposed to an antimicrobial agent. The resistance rates to antimicrobials in companion animals have risen considerably. Studies of local antimicrobial susceptibility profiles are needed as well as education and warning about the use of tests for the identification and susceptibility of pathogenic bacterial strains. The aim of this study was to identify the main antimicrobial resistance in clinical samples of dogs, and to detect multidrug-resistant strains of importance to public health.Materials, Methods & Results: Bacterial pathogens of 77 dog infections were isolated and their sensitivity profile to antimicrobials was determined. One hundred bacterial isolates were identified. Of these, 61 were Gram-positive (55 Staphylococcus spp., 4 Enterococcus spp. and 2 Streptococcus spp.) and 39 Gram-negative (36 fermenters and 3 non-fermenters). Seventy-nine isolates were considered multiresistant following individual assessment of drugs, and 85 following the evaluation of classes. Only 3 were sensitive to all drugs. Four isolates were resistant to all classes and only sensitive to some antibiotics. Of the 55 samples of Staphylococcus spp., 36 (65.45%) were identified as phenotypically MRS. Two isolates of Enterococcus spp. were resistant to vancomycin (VRE). Also 66.67% (26/39) of the samples were positive for the presumptive test for ESBL. For the MRS-positive isolates detected in this study, chloramphenicol was the antimicrobial that showed superior sensitivity in 74.29% of the cases (27/36); therefore it is considered the most appropriate for treatment of this type of micro-organism. In case of aminoglycosides, when their resistance was checked in MRS isolates, all resistance percentages increased, implying a limited use of this class for such a type of multi-resistant micro-organism. Contrarily, in case of ESBL, a superior sensitivity was observed towards MRS isolates, thus making them a prime treatment choice for the infection caused by these micro-organisms.Discussion: Literature have reported a gradual increase in multidrug resistance towards antimicrobial agents in veterinary medicine over the past decades. In this study, 64% of multiresistant strains were considered of significant importance, notably MRS (36), VRE (2) and ESBL (26). The early identification of pathogens in animals has become an important step in order to minimize the transmission of antibacterial resistance. The increase in the number of multidrug-resistant bacteria in animals and humans demonstrates the need to develop and implement measures in order to monitor and control the spread of this resistance. It is possible that the increased drug resistance is linked to the constant exposure to these drugs and the subsequent selective pressure, causing the transfer of resistant genes between strains. Carbapenems and glycopeptides should be used with caution in veterinary medicine in order to prevent such processes of selection that develop resistance in micro-organisms to these two classes, which can result in cross-resistance between animals and humans and create obstacles in the treatment of patients, especially for the two drugs mentioned, since they are important for the treatment of nosocomial infections in humans. The resistance percentage towards fluoroquinolones was identified to be higher in Gram-positive isolates, particularly in MRS, which showed 75% resistance against this class (according to the CLSI, resistance to one fluoroquinolone antimicrobial agent provides resistance to other antimicrobials of this class). For ESBL isolates, the resistance was shown to be 50%. The resistance towards the fluoroquinolones and aminoglycosides class can be associated with the expression of the genes that produce ESBL.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The results were submitted for descriptive analysis in order to calculate absolute and relative frequencies [27].…”

Section: Methodsmentioning

confidence: 99%

Section: Introducionmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial Resistance Phenotypic Profile of Isolates from Clinical Infections in Dogs

Sfaciotte

Coronel

Snak

et al. 2017

Acta Scientiae. Vet.

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“…(PLESSAS et al, 2013) e Staphylococcus Aureus resistente à meticilina (STULL; WEESE, 2015). Estudos realizados em hospitais veterinários (SFACIOTTE et al, 2014), relataram a ocorrência de resistência bacteriana a antibióticos como penicilinas, amoxicilina, ampicilina, entre outros. Tal panorama está intimamente relacionado à utilização profilática e empírica de antimicrobianos no pré e trans-cirúrgico, o que aumenta a pressão de seleção a resistência (KOHL et al, 2016).…”

Section: Fatores De Risco Trans-operatóriosunclassified

Infecção Relacionada À Assistência À Saúde Em Cirurgias Limpas

Silva¹,

Coelho²,

Barbosa³

et al. 2018

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RESUMO Infecção relacionada com assistência à saúde (IRAS) é atribuída aos quadros adquiridos tanto no momento da admissão em ambientes de atenção à saúde, quanto durante a internação, e até mesmo após alta médica, sendo assim, correlacionada com a permanência hospitalar. Na rotina cirúrgica veterinária, pacientes tanto hígidos quanto imunocomprometidos, tornam-se susceptíveis. Ressalva-se, que a ferida cirúrgica infectada por microrganismos endógenos ou exógenos, com classificação de multirresistentes ou não, pode desencadear quadros sépticos. Denomina-se infecção no sítio cirúrgico (ISC), a colonização da ferida cirúrgica por microrganismos, sendo sua ocorrência no pós-operatório, uma das principais complicações relacionadas à IRAS. Os principais fatores que podem levar a ISC estão ligados ao pré, trans e pós-operatório. Os médicos veterinários que atuam diretamente em ambiente hospitalar, devem estar preparados para prevenir e tratar casos de ISC de maneira integrada e dinâmica. Entre as ferramentas importantes nesse processo, destacam-se os programas multidisciplinares de Antibiotic Stewardship associados ao desenvolvimento de técnicas diagnósticas rápidas e ao incentivo de medidas consagradas para o controle da infecção hospitalar. Objetiva-se com o presente trabalho ressaltar os principais fatores de risco, medidas profiláticas, e o programa Antibiotic Stewardship, para o controle da ISC.

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Antimicrobial resistance evaluation of bacteria isolated from infections in small animals in the Umuarama region, Paraná

et al. 2020

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Bacterial resistance is shown to be an inevitable side effect due to the excessive use of antibiotics, becoming a significant concern worldwide. Knowledge of regional bacterial resistance profiles enables the development of site-specific infection control practices, making conscious and moderate use of commercially available antibiotics. The aim of this study was the retrospective evaluation of the antimicrobial resistance profile of bacteria isolated from companion animal infections in the region of Umuarama/PR, from 2013 to 2017. This research was performed by analyzing the database belonging to the “Laboratório de Microbiologia Animal” at the “Universidade Estadual de Maringá” (UEM). Staphylococcus spp. represented 45.53% of the bacteria isolated from clinical infections in small animals in the period and place evaluated, followed by enterobacteria (34.04%), non-fermenting Gram-negative bacilli (NFGNB, 11.06%) and Streptococcus/Enterococcus (9.36%). A high number of antimicrobial resistance to antibiotics used in veterinary medicine was found. The lowest resistances associated with the best impact factor values were found for aminoglycosides, especially amikacin, chloramphenicol, and fluoroquinolones (norfloxacin and ciprofloxacin). Intermediate results were found for sulbactam-associated ampicillin, ceftriaxone, amoxicillin-clavulanic acid, and enrofloxacin. According to the number of resistant antimicrobial drugs, 64.26% (151/235) of the isolates were classified as multidrug-resistant, being 15.32% extensively resistant. Considering the resistance to antimicrobial classes, 68.94% (162/235) of the isolates were classified as multiresistant, being 19.15% extensively resistant. No bacterial strains were characterized as pan-resistant, but ten bacteria were resistant to all classes tested, with isolated susceptibility to certain drugs. Through the evaluation of resistance profiles found in the period and place studied and relevant literature, it is clear that there is a growing increase in the number of multiresistant bacteria among domestic animals which characterizes a serious risk to public health. The therapeutic arsenal is becoming increasingly diminished, and there is more difficulty in empirical drug selection, making antimicrobial susceptibility testing essential for more specific selection in antimicrobial therapy. Educational measures on the conscious use of antibiotics, infection control, and prevention of local specific zoonoses need to be instituted for the knowledge of health professionals and general access of the population.

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Perfil De Resistência Antimicrobiana De Isolados Bacterianos De Afecções Clínicas Do Hospital Veterinário Da Universidade Estadual De Maringá

Cited by 5 publications

References 4 publications

Antimicrobial Resistance Phenotypic Profile of Isolates from Clinical Infections in Dogs

Antimicrobial Resistance Phenotypic Profile of Isolates from Clinical Infections in Dogs

Infecção Relacionada À Assistência À Saúde Em Cirurgias Limpas

Antimicrobial resistance evaluation of bacteria isolated from infections in small animals in the Umuarama region, Paraná

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