Existent literature has evident that dysfunctional attitudes and automatic thoughts are associated with mental health difficulties. The present study attempts to investigate the relationship between dysfunctional attitudes and automatic thoughts and examining gender differences across both variables. A sample of 27 male and 53 females were collected from different universities of Rawalpindi and Islamabad, Pakistan. Dysfunctional attitudes of students were measured by the short form of Dysfunctional Attitude Scale (DAS) while automatic thoughts were measured by Automatic thoughts Questionnaire-negative (ATQ). Results indicate that dysfunctional attitudes are not associated with automatic thoughts (p > 0.001), except confusion escape (r = .348, p < 0.001). We found that male university students experience more dysfunctional attitudes and confusion escape subscale of automatic thoughts as compared to female students (p < 0.001). However, there were no gender differences in negative self-concept, personal maladjustment, loneliness, and giving up subscales of automatic thoughts. Result suggests that students should be trained to control these thought negative patterns and resultant psychopathologies.