Perez-Chacon et al=SUPPLEMENTARY INFORMATION from Indole-3-Carbinol Synergizes with and Restores Fludarabine Sensitivity in Chronic Lymphocytic Leukemia Cells Irrespective of p53 Activity and Treatment Resistances

Abstract

<p>Supplementary Table 1. Statistical analysis of the effect of the combinatory I3C/F-ara-A treatment versus single I3C or F-ara-A treatments. Supplementary Table 2. Combination index (CI) for effective dose (ED) 50, 75 and 90 for I3C combined with F-ara-A, vincristine or chlorambucil and dose reduction index (DRI) for these drugs in the combined treatment with I3C. Supplementary Figure S1. I3C is highly cytotoxic to CLL cells while having reduced cytotoxicity against PBMCs from healthy donors. Supplementary Figure S2. I3C and F-ara-A show strong synergy against CLL cells. Supplementary Figure S3. I3C cytotoxicity and synergy with F-ara-A is fully functional in the presence of human serum. Supplementary Figure S4. Effect of I3C, F-ara-A and the I3C/F-ara-A combination treatment on the expression of proteins involved in the control of apoptosis. Supplementary Figure S5. A. Effect of I3C, F-ara-A and the combined F-ara-A/I3C treatment on mitochondrial depolarization (ΔΨm). B. Effect of I3C, F-ara-A and the combined F-ara-A/I3C treatment on reactive oxygen species (ROS) production. Supplementary Figure S6. I3C has reduced toxicity in T cells from CLL patients compared to CLL cells.</p>