Percutaneous needle muscle biopsy in the diagnosis of neuromuscular disorders in children. Histological, histochemical and electron microscopic studies

Fukuyama, Yukio; Suzuki, Yoko; Hirayama, Yoshito; Harada, Junko; Ohsawa, Makiko; Saito, Kayoko; Ochiai, Ekukoi; Nakane, Atsuko

doi:10.1016/s0387-7604(81)80050-2

Cited by 13 publications

(14 citation statements)

References 22 publications

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“…The biopsy itself took between 2 and 3 s to perform and resulted in minimal discomfort. In over 200 previously studied patients there have been minimal or no complications [22][23][24]. There were no complications from the biopsy proce dure itself in any of our CFS patients or controls.…”

Section: Methodsmentioning

confidence: 64%

“…21], and in the work of Behan et al [8][9][10][11] the EM specimens were of this size. The Tru-Cut needle has been used in previous investigations and has given tissue samples with excellent histologic and ultrastructural qualities [22][23][24], After the procedure, pressure was placed on the site and after several minutes a band-aid was applied. As reported in many pre vious studies, the needle muscle biopsies were very' well tolerated by all of our CFS patients and controls.…”

Section: Methodsmentioning

confidence: 99%

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Electron-Microscopic Investigation of Muscle Mitochondria in Chronic Fatigue Syndrome

Plioplys

1995

Neuropsychobiology

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Patients with chronic fatigue syndrome (CFS) suffer from disabling physical and mental fatigue. Abnormalities in mitochondrial function can lead to fatigue and weakness. Ultrastructural mitochondrial abnormalities have been reported to be present in CFS patients. We obtained percutaneous needle muscle biopsies from 15 CFS patients and 15 age- and sex-matched controls. We investigated previously reported ultrastructural abnormalities in CFS: subsarcolemmal mitochondrial aggregates, intermyofibrillar mitochondrial aggregates, mitochondrial circumference, area, pleomorphism and the presence of compartmentalization of the inner mitochondrial membrane. All of the steps of tissue processing, electron microscopy and data abstracting and analysis were performed in a totally blinded fashion. All of our data were rigorously quantified. We found no difference in any of these studied parameters between CFS patients and controls. Although there is no ultrastructural mitochondrial abnormality in CFS patients, other lines of evidence suggest the presence of a possible functional mitochondrial abnormality.

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Section: Methodsmentioning

confidence: 64%

Section: Methodsmentioning

confidence: 99%

Electron-Microscopic Investigation of Muscle Mitochondria in Chronic Fatigue Syndrome

Plioplys

1995

Neuropsychobiology

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“…Potential limitations include the small sample size, which may prevent determination of a focal disorder, interfere with quantification of fiber types, or provide insufficient material for metabolic studies. [16][17][18][19] Good correlation of results is seen in comparisons between NMB and open biopsy performed in the same setting at the same incision site. 17,19 NMB has been shown to yield a definitive diagnosis in patients with a prior open muscle biopsy.…”

Section: -14mentioning

confidence: 94%

“…[16][17][18][19] Good correlation of results is seen in comparisons between NMB and open biopsy performed in the same setting at the same incision site. 17,19 NMB has been shown to yield a definitive diagnosis in patients with a prior open muscle biopsy. 17 Postulated reasons for repeat NMB include insufficient material, clinical suspicion of disease in setting of non-diagnostic biopsy, or need for additional material for mitochondrial DNA screening.…”

Section: -14mentioning

confidence: 94%

Role of repeat skeletal muscle biopsy: How useful is it?

Goutman

Prayson

2013

Muscle and Nerve

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“…Other authors have reported that percutaneous biopsies produce samples of comparable quality to open biopsies, are less traumatic and require few resources 4,[7][8][9] . When performed by someone familiar with the technique, the volume of muscle obtained is rarely insufficient for microscopic analysis 4,9,10 with no compromise of histological or histochemical features 11,12 .…”

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confidence: 99%

Percutaneous Muscle Biopsies: Review of 900 Consecutive Cases at London Health Sciences Centre

Derry

Nicolle

Keith-Rokosh

et al. 2009

Can. j. neurol. sci.

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Muscle biopsies are an important tool in the diagnosis and management of neuromuscular disorders 1,2 . The biopsies can be performed open or by percutaneous means. Open biopsy benefits from the opportunity to directly visualize the muscle, which is typically held in a muscle biopsy clamp which in turn facilitates orientation (for histopathological examination) and maintains the fibres in a fixed length optimal for ultrastructural examination. If necessary, larger samples can be obtained with an open biopsy and the opportunity to sample peripheral nerve exists.Percutaneous (needle) biopsies were likely first performed in the mid 1860's, at which time Charriere and Duchenne described the procedure using a trocar-style instrument 3 . Modern muscle biopsy trocars are similar in design. ABSTRACT:Objective: In the present study we review our experience with 900 consecutive percutaneous muscle biopsies over the period 1993 to 2007. We examined the advantages and limitations of the procedure, biopsy site preferences, diagnostic range, frequency of diagnoses and quality of histopathology. Demographics, referral patterns and patients' perceptions of the procedure were also assessed. Methods: Cases were identified through the London Health Sciences Centre Department of Pathology database. Standard biopsy procedures were followed using a manual trocar style instrument. With a neuropathology technologist in attendance at all biopsies, biopsies were oriented in the fresh state and snap frozen. Results: Most referrals for muscle biopsy were from neuromuscular neurologists. The procedure was found to be efficient, well-tolerated and produced high quality specimens in all diagnostic categories. No major complications occurred. Failure to obtain an adequate tissue sample, although uncommon (<2%), was usually due to marked obesity, edema or muscle wasting. Bleeding at the site was rarely problematic and no wound infections were reported. Conclusions: Needle muscle biopsies represent an efficient alternative to open biopsies when peripheral nerve sampling is not required and when large tissue samples are not needed for extensive biochemical analyses.RÉSUMÉ: Biopsie musculaire percutanée : revue de 900 cas consécutifs au London Health Sciences Centre. Objectif : Nous avons revu notre expérience concernant 900 cas consécutifs de biopsies musculaires percutanées effectuées entre 1993 et 2007. Nous avons examiné les avantages et les limites de cette intervention, l'endroit choisi pour faire la biopsie, la plage diagnostique, la fréquence des diagnostics et la qualité de l'histopathologie. Nous avons également analysé les données démographiques, les modalités de référence et les perceptions des patients concernant l'intervention. Méthodes : Les cas ont été identifiés au moyen de la base de données du département de pathologie du London Health Sciences Centre. Un trocart manuel a été utilisé et la procédure standard de biopsie a été suivie. Un technicien en neuropathologie était présent au moment de chacune des biopsies et il prenait cha...

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Percutaneous needle muscle biopsy in the diagnosis of neuromuscular disorders in children. Histological, histochemical and electron microscopic studies

Cited by 13 publications

References 22 publications

Electron-Microscopic Investigation of Muscle Mitochondria in Chronic Fatigue Syndrome

Electron-Microscopic Investigation of Muscle Mitochondria in Chronic Fatigue Syndrome

Role of repeat skeletal muscle biopsy: How useful is it?

Percutaneous Muscle Biopsies: Review of 900 Consecutive Cases at London Health Sciences Centre

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