Percutaneous exposure of adjuvant oil causes arthritis in DA rats

Abstract

SUMMARYThe arthritogenic properties of adjuvant oil upon percutaneous administration in DA rats was investigated. Groups of rats were administered single or repeated percutaneous applications of Freund's incomplete adjuvant (FI A) or olive oil on shaved skin with or without prior abrasion of the skin. Control rats were shaved and abrased only. A transient arthritis developed in 8/16 animals after repeated applications of FIA on abrased skin. The incidence of arthritis increased to 7/8 animals when FIA was repeatedly administered via filter paper on abrased skin and covered with a bandage. Histological examination of the arthritic joints showed proliferation of the synovial lining layer, infiltration of mononuclear cells and polymorphonuclear cells in the subsynovial tissue. Some bone and cartilage destruction was also seen. Repeated treatment with olive oil on abrased skin induced joint swelling in 3/15 animals, which did not. however, correspond to any microscopically observable signs of inflammation. Also, a single application of FIA on abrased skin or repeated applications of Fi A without abrasion induced arthritis, although with low penetration, whereas control animals had no clinical signs of joint involvement. These findings demonstrate that percutaneous administration of adjuvant oil can cause arthritis in genetically susceptible animals.

Evaluation Of The Anti-Arthritic Activity Of The Hydroethanolic Leaf Extract Of <i>Alchornea Cordifolia</i> In Rats

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Effector T cells in rheumatoid arthritis: Lessons from animal models

“…One possible explanation for the induction of arthritis following immunisation with adjuvants is that there is an increase in the activity of APC [56], leading to the presentation to autoreactive T cells of a hitherto unrecognised or ''sequestered'' endogenous antigen. The possibility that human RA could also be initiated by exposure to environmental factors with adjuvant-like activity has been highlighted by experiments in which arthritis was found to be induced in susceptible DA rats by percutaneous exposure of adjuvant oils [57], or even a mineral oil-containing cosmetic product [58]. Proteoglycan-induced arthritis (PGIA) is a model of polyarthritis induced by the injection of human cartilage proteoglycan plus adjuvant intraperitoneally genetically susceptible mice (BALB/c and C3H).…”

Protective effect of latex of Calotropis procera in Freund's Complete Adjuvant induced monoarthritis

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