Microwave ablation (MWA) status monitoring in real time plays a key role in assessment of therapeutic e®ectiveness. As a novel real-time assessment method, near infrared spectroscopy (NIRs) was used to evaluate the ablation e±cacy. MWA experiments were carried out on in vitro porcine livers. An optical measurement system for biological tissue is developed by our lab to monitor reduced scattering coe±cient ( 0 s ) at 690 nm of the coagulation zones. It is noted that 0 s of liver tissue, which increases as the liver tissue being ablated, is clearly related with the coagulation status. 0 s of normal tissue and coagulated tissue is 3-5 and 17-19 cm À1 , respectively. Continuous changes of 0 s demonstrate that optical parameter can be used as an e±cacy evaluation factor because it essentially indicates the degree of thermal damage. Compared with temperature, optical parameter is more sensitive and accurate, which is promising for real-time therapeutic e±cacy assessment in MWA.