Perchlorate bio-reduction in a methane-based membrane biofilm reactor in the presence and absence of oxygen

Wu, Mengxiong; Luo, Jing; Hu, Shihu; Yuan, Zhiguo; Guo, Jianhua

doi:10.1016/j.watres.2019.04.008

Cited by 36 publications

(22 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although it is close to impossible to predict the actual substrates of the NarG LFLS (or PcrA LFLS ), the phylogenetic analysis is, at least, suggestive of the potential utilisation of (per)chlorate. The oxidation of methane linked to the bioreduction of perchlorate has been proposed a number of times in the past ( Luo et al, 2015 ; Xie et al, 2018 ; Wu et al, 2019 ). Recent works ( Xie et al, 2018 ; Wu et al, 2019 ) have suggested the possible involvement of ANME archaea in (per)chlorate reduction based on chemical and amplicon data analyses, an assumption considered more likely given that nitrate was rarely measured in noticeable concentrations in the LFLS sampling trench ( Vázquez-Campos et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…The oxidation of methane linked to the bioreduction of perchlorate has been proposed a number of times in the past ( Luo et al, 2015 ; Xie et al, 2018 ; Wu et al, 2019 ). Recent works ( Xie et al, 2018 ; Wu et al, 2019 ) have suggested the possible involvement of ANME archaea in (per)chlorate reduction based on chemical and amplicon data analyses, an assumption considered more likely given that nitrate was rarely measured in noticeable concentrations in the LFLS sampling trench ( Vázquez-Campos et al, 2017 ). Indeed, nitrate concentrations were below the detection limit (<0.01 μM) before any ‘ Ca.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site

et al. 2021

View full text Add to dashboard Cite

During the 1960s, small quantities of radioactive materials were co-disposed with chemical waste at the Little Forest Legacy Site (LFLS, Sydney, Australia). The microbial function and population dynamics in a waste trench during a rainfall event have been previously investigated revealing a broad abundance of candidate and potentially undescribed taxa in this iron-rich, radionuclide-contaminated environment. Applying genome-based metagenomic methods, we recovered 37 refined archaeal MAGs, mainly from undescribed DPANN Archaea lineages without standing in nomenclature and ‘Candidatus Methanoperedenaceae’ (ANME-2D). Within the undescribed DPANN, the newly proposed orders ‘Ca. Gugararchaeales’, ‘Ca. Burarchaeales’ and ‘Ca. Anstonellales’, constitute distinct lineages with a more comprehensive central metabolism and anabolic capabilities within the ‘Ca. Micrarchaeota’ phylum compared to most other DPANN. The analysis of new and extant ‘Ca. Methanoperedens spp.’ MAGs suggests metal ions as the ancestral electron acceptors during the anaerobic oxidation of methane while the respiration of nitrate/nitrite via molybdopterin oxidoreductases would have been a secondary acquisition. The presence of genes for the biosynthesis of polyhydroxyalkanoates in most ‘Ca. Methanoperedens’ also appears to be a widespread characteristic of the genus for carbon accumulation. This work expands our knowledge about the roles of the Archaea at the LFLS, especially, DPANN Archaea and ‘Ca. Methanoperedens’, while exploring their diversity, uniqueness, potential role in elemental cycling, and evolutionary history.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Although it is close to impossible to predict the actual substrates of the NarG LFLS (or PcrA LFLS ), the phylogenetic analysis is, at least, suggestive of the potential utilisation of (per)chlorate. The oxidation of methane linked to the bioreduction of perchlorate has been proposed a number of times in the past (Luo et al, 2015; Xie et al, 2018; Wu et al, 2019). Recent works (Xie et al, 2018; Wu et al, 2019) have suggested the possible involvement of ANME archaea in (per)chlorate reduction based on chemical and amplicon data analyses, an assumption considered more likely given that nitrate was rarely measured in noticeable concentrations in the LFLS sampling trench (Vázquez-Campos et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Genomic insights into the Archaea inhabiting an Australian radioactive legacy site

Vázquez-Campos

Kinsela

Bligh

et al. 2019

Preprint

View full text Add to dashboard Cite

18During the 1960s, small quantities of radioactive materials were co-disposed with chemical waste at 19 the Little Forest Legacy Site (LFLS, Sydney, Australia). The microbial function and population 20 dynamics during a rainfall event using shotgun metagenomics has been previously investigated. This 21 revealed a broad abundance of candidate and potentially undescribed taxa in this iron-rich, 22 radionuclide-contaminated environment. 23Here, applying genome-based metagenomic methods, we recovered 37 refined archaeal bins (≥50% 24 completeness, ≤ 10% redundancy) from 10 different major lineages. They were, for the most part, 25 included in 4 proposed lineages within the DPANN supergroup (LFWA-I to IV) and 26 Methanoperedenaceae. 27The new Methanoperedens spp. bins, together with previously published data, suggests a potentially 28 widespread ability to use nitrate (or nitrite) and metal ions as electron acceptors during the anaerobic 29 oxidation of methane by Methanoperedens spp. 30 While most of the new DPANN lineages show reduced genomes with limited central metabolism 31 typical of other DPANN, the candidate species from the proposed LFWA-III lineage show some 32 unusual features not often present in DPANN genomes, i.e. a more comprehensive central metabolism 33 and anabolic capabilities. While there is still some uncertainty about the capabilities of LFW-121_3 34 and closely related archaea for the biosynthesis of nucleotides de novo and amino acids, it is to date 35 the most promising candidate to be a bona fide free-living DPANN archaeon. 36 environments such as rice fields and ocean floors before it is released into the atmosphere [12]. Our 64 previous research showed that the archaeal community in the LFLS trenches was mainly composed of 65 methanogens and anaerobic methane oxidisers (ANME-2d) from the Methanomicrobia class and 66 DPANN archaea [3]. While they constituted a minor component of the community, they still may 67 have an important role in facilitating redox cycling and therefore impacting upon contaminant 68 mobilisation. 69 Here we describe the analysis of 37 new archaeal genomes with different degrees of completeness, 70 derived from samples collected in a legacy radioactive waste trench at the LFLS during a redox 71 cycling event. We present evidence of four new DPANN lineages and six non-conspecific 72 Methanoperedens sp. genomes, while exploring their potential role in the biogeochemical cycles and 73 uniqueness. 74 Material and Methods 75 Data source and experimental details 76 Raw sequencing reads from ENA project PRJEB14718 [3] were reanalysed, this time using 77 genome-based metagenomic methodologies in order to better understand the contributions of the 78 Archaea in the LFLS groundwater to the biogeochemistry of the site. Briefly, samples were collected 79 in triplicate over a period of 47 days at 4 time-points (0, 4, 21 and 47 days) after an intense rainfall 80 event that completely filled the trench. Thorough chemical and radiochemical analyses were 81 conducted on the samples in...

show abstract

“…MBRs overcome diffusional limitations by promoting high masstransfer rates of sparingly soluble gaseous substrates to the biomass. MBRs have been reported for denitrification (Lee et al, 2019) and removal of perchlorate (Wu et al, 2019), chromate (Luo et al, 2019), vanadate (Wang et al, 2019), selenate (Shi et al, 2020), etc. from contaminated wastewater and groundwater using methanotrophs.…”

Section: Mass Transfer and Solubility Limitations In Methanotroph Basmentioning

confidence: 99%

Biotransformation of Methane and Carbon Dioxide Into High-Value Products by Methanotrophs: Current State of Art and Future Prospects

2021

View full text Add to dashboard Cite

Conventional chemical methods to transform methane and carbon dioxide into useful chemicals are plagued by the requirement for extreme operating conditions and expensive catalysts. Exploitation of microorganisms as biocatalysts is an attractive alternative to sequester these C1 compounds and convert them into value-added chemicals through their inherent metabolic pathways. Microbial biocatalysts are advantageous over chemical processes as they require mild-operating conditions and do not release any toxic by-products. Methanotrophs are potential cell-factories for synthesizing a wide range of high-value products via utilizing methane as the sole source of carbon and energy, and hence, serve as excellent candidate for methane sequestration. Besides, methanotrophs are capable of capturing carbon dioxide and enzymatically hydrogenating it into methanol, and hence qualify to be suitable candidates for carbon dioxide sequestration. However, large-scale production of value-added products from methanotrophs still presents an overwhelming challenge, due to gas-liquid mass transfer limitations, low solubility of gases in liquid medium and low titer of products. This requires design and engineering of efficient reactors for scale-up of the process. The present review offers an overview of the metabolic architecture of methanotrophs and the range of product portfolio they can offer. Special emphasis is given on methanol biosynthesis as a potential biofuel molecule, through utilization of methane and alternate pathway of carbon dioxide sequestration. In view of the gas-liquid mass transfer and low solubility of gases, the key rate-limiting step in gas fermentation, emphasis is given toward reactor design consideration essential to achieve better process performance.

show abstract

Perchlorate bio-reduction in a methane-based membrane biofilm reactor in the presence and absence of oxygen

Cited by 36 publications

References 39 publications

Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site

Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site

Genomic insights into the Archaea inhabiting an Australian radioactive legacy site

Biotransformation of Methane and Carbon Dioxide Into High-Value Products by Methanotrophs: Current State of Art and Future Prospects

Contact Info

Product

Resources

About