Perchlorate and nitrate reductase activity in the perchlorate-respiring bacterium perclace

Giblin, Tara; Frankenberger, W. T.

doi:10.1078/0944-5013-00111

Cited by 56 publications

(42 citation statements)

References 14 publications

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“…It has been found that some PRB, for example Dechloromonas agitata CKB , can respire using perchlorate but not nitrate. In a PRB capable of denitrification (strain perclace), it was found that nitrate and perchlorate reductases were located in different cell fractions (membrane and periplasmic fractions, respectively), indicating that these two enzymes were separate (Giblin and Frankenberger, 2001). Further evidence of completely separate denitrifying and perchlorate pathways was provided by Xu et al (2002).…”

Section: Clomentioning

confidence: 78%

Microbial Degradation of Perchlorate: Principles and Applications

Xu¹,

Song²,

Min³

et al. 2003

Environmental Engineering Science

163

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Perchlorate (ClO 42 ) release into the environment has occurred primarily in association with its manufacture and use in solid rocket propellant. When released into groundwater, perchlorate can spread over large distances because it is highly soluble in water and adsorbs poorly to soil. Two proven techniques to remove perchlorate from drinking water are anaerobic biological reactors and ion exchange. In this review, we focus on the application of microbiological systems for degrading perchlorate. Some bacteria can use perchlorate as an electron acceptor while oxidizing a large range of substrates. Perchlorate-respiring bacteria (PRB) are widely distributed in the environment, and are enriched at perchlorate-contaminated sites. We review the pathways by which PRB degrade perchlorate, and the different biological treatment processes that have been developed to remove perchlorate from water sources. We also discuss the effects of alternate electron acceptors in the water, such as oxygen and nitrate, on perchlorate removal. Although many different biological treatment systems using PRB have so far only been proven at the bench scale, all pilot scale tests performed to date with a few of these systems have been successful. The success of these perchlorate bioreactor tests indicates that biological treatment is a suitable method for soil remediation and water treatment of perchlorate-contaminated water.

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Section: Clomentioning

confidence: 78%

Microbial Degradation of Perchlorate: Principles and Applications

Xu¹,

Song²,

Min³

et al. 2003

Environmental Engineering Science

163

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“…The main source of chlorate contamination is chlorine dioxide (ClO 2 ) bleaching in the pulp and paper industry (8), and the most efficient remediation techniques for both chlorate and perchlorate are based on degradation performed by microorganisms (13,33,43). More than 40 bacterial strains that are able to grow by dissimilatory perchlorate or chlorate reduction have been isolated (2,6,10,13,15,18,21,22,24,36,37,39,40). They are phylogenetically diverse, with members in the Alpha-, Beta-, Gamma-, and Epsilonproteobacteria (13,15).…”

mentioning

confidence: 99%

Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans

Lindqvist

Johansson

Nilsson

et al. 2012

Appl Environ Microbiol

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ABSTRACTThe ability of microorganisms to perform dissimilatory (per)chlorate reduction is, for most species, known to be oxygen sensitive. Consequently, bioremediation processes for the removal of oxochlorates will be disturbed if oxygen is present. We measured the expression of chlorite dismutase and chlorate reductase in the presence of different terminal electron acceptors in the chlorate reducerIdeonella dechloratans. Enzyme activity assays and mRNA analyses by real-time quantitative reverse transcription (qRT)-PCR were performed on cell extracts from cells grown aerobically with and without chlorate and on cells grown anaerobically in the presence of chlorate. Our results showed that both chlorite dismutase and chlorate reductase are expressed during aerobic growth. However, transfer to anaerobic conditions with chlorate resulted in significantly enhanced enzyme activities and mRNA levels for both enzymes. Absence of oxygen was necessary for the induction to occur, since chlorate addition under aerobic conditions produced neither increased enzyme activities nor higher relative levels of mRNA. For chlorite dismutase, the observed increase in activity was on the same order of magnitude as the increase in the relative mRNA level, indicating gene regulation at the transcriptional level. However, chlorate reductase showed about 200 times higher enzyme activity in anaerobically induced cells, whereas the increase in mRNA was only about 10-fold, suggesting additional mechanisms influence the enzyme activity.

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“…These results showed that the optimal mass concentration ratio of acetate to perchlorate was approximately 5 (molar ratio of 8.5). This molar ratio of PMJ was much larger than previously-isolated PCRBs (molar ratio of 1∼3.2) (Giblin and Frankenberger 2001;Logan et al 2001;Nerenberg et al 2006;Rikken et al 1996;Waller et al 2004;Xu et al 2004), but these previously reported acetate concentrations were merely culture conditions. Besides, the results were similar to those of recent reports (Xu et al 2015;Zhu et al 2016) in that Azospira sp.…”

Section: Concentration Effects Of Perchlorate and Acetatementioning

confidence: 84%

“…KJ (Bardiya and Bae 2011;Xu et al 2015) in which nitrate reduction was inhibited by the perchlorate. For another case, Giblin and Frankenberger (2001) (Fig. 6).…”

Section: Discussionmentioning

confidence: 95%

“…− , but most studies on genus Azospira in PCRB have shown that perchlorate reduction was inhibited by nitrate (Bardiya and Bae 2011;Giblin and Frankenberger 2001;Xu et al 2015). On the contrary, perchlorate was more rapidly reduced than nitrate by PMJ, which suggested that preferred electron acceptor was perchlorate rather than nitrate (Fig.…”

Section: Competition Among Electron Acceptorsmentioning

confidence: 94%

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A novel perchlorate- and nitrate-reducing bacterium, Azospira sp. PMJ

Nam

Ventura

Yeom

et al. 2016

Appl Microbiol Biotechnol

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A novel perchlorate-reducing bacterium (PCRB), PMJ, was isolated from the mixed liquor suspended solids in the aerobic tank of a wastewater treatment plant. The 16S ribosomal RNA (rRNA), perchlorate reductase, and chlorite dismutase gene sequences revealed that PMJ belonged to the genus Azospira. PMJ was removed high-strength (700 mg/L) perchlorate and also removed low-strength (≤50 mg/L) perchlorate below the detection limit (2 μg/L) when acetate was used as a sole and carbon source. The maximum specific perchlorate utilization rate, q max, was 0.96 mg ClO4 (-)/mg dry cell weight day, and the half-saturation constant, K S , was lower than 0.002 mg ClO4 (-)/L. PMJ also utilized inorganic electron donors [(H2, S(0), and Fe(II)] with perchlorate as an electron acceptor. Perchlorate reduction by PMJ was completely inhibited by oxygen and chlorate but was not inhibited by nitrate. In the presence of similar concentrations (100∼140 mg/L) of nitrate and perchlorate, PMJ simultaneously removed both electron acceptors. Therefore, it was concluded that the strains PMJ might possess separate pathways for perchlorate and nitrate reduction. These results indicated that Azospira sp. PMJ could be efficiently used for treating perchlorate-contaminated groundwater and wastewater because many of these water bodies are known to contain both perchlorate and nitrate. In addition, low K S value and autotrophic perchlorate reduction of PMJ might be useful to design the biological treatment systems.

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Perchlorate and nitrate reductase activity in the perchlorate-respiring bacterium perclace

Cited by 56 publications

References 14 publications

Microbial Degradation of Perchlorate: Principles and Applications

Microbial Degradation of Perchlorate: Principles and Applications

Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans

A novel perchlorate- and nitrate-reducing bacterium, Azospira sp. PMJ

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