Perbandingan Metode Uji Gula Pereduksi Dalam Penentuan  Aktivitas ?-L-Arabinofuranosidase Dengan Substrat  Janur Kelapa (Cocos Nucifera)

Pratiwi, YuliaRatna; Ratnayani, Oka; Wirajana, I Nengah

doi:10.24843/jchem.2018.v12.i02.p07

Cited by 9 publications

(8 citation statements)

References 3 publications

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“…Standard glucose solution was made according to the method employed by [9] with slight modification [10 mg glucose/100ml]. Sugar content was determined using the Nelson -Somogyi method.…”

Section: Reducing Sugar Contentmentioning

confidence: 99%

QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND THE ANTIOXIDANT PROPERTIES OF THE METHANOL EXTRACT FROM THE LEAVES OF Solanum americanum.

Nwabiani¹,

Ogbuagu²,

Okerulu³

2023

JCSN

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This study estimated the quantity of some phyto- compounds as well as the antioxidants activities of the methanol leave extract of Solanum americanum [S.a]. The quantitative phytochemical analysis were carried out using gravimetric and spectroscopic method while the antioxidant activities were assayed using free radical scavenging activities of DPPH, Nitric oxide, hydrogen peroxide and ABTS. The quantitative analysis showed that the phenolic content of the leaves to have highest concentration at 62mg/100g alkaloids 60mg/100g, total flavonoids and tannins 48 and 45mg/100g respectively. The extract showed inhibitory potential against DPPH free radical, the inhibitory percentages vary from 33.68±0.74 to 80.83±1.10 at 100µg/ml for the methanol extract of the plant Solanum americanum to 48.19±0.73 to 84.59±0.91 at 100µg/ml for the standard [ascorbic acid]. The maximum NO scavenging activity of the plant extract to ascorbic acid are 77.89±0.69 to 89.19±0.35 at a concentration of 100µg/ml. The IC50 value are 47.79 for the inhibition of the plant extract while that of the standard is 27.80. ABTS assay free radical scavenging percent inhibition has an increased concentration from 25-100 μg/ml at 33.75±0.87- 71.92±0.15. H2O2 radicals scavenging activity inhibition percentage of methanol leaf extract of the plant Solanum americanum was from 53.82±0.37 to 76.89±0.32 at 25-100μg/ml as compared to the standard with inhibition percentage from 78.35±0.79 to 95.18±0.37. The quantitative analysis showed that the phenolic content has the highest concentration, hence has the most antioxidant activity also that the plant has strong free radical scavenging activities.

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“…Standard glucose solution was made according to the method employed by [9] with slight modification [10 mg glucose/100ml]. Sugar content was determined using the Nelson -Somogyi method.…”

Section: Reducing Sugar Contentmentioning

confidence: 99%

QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND THE ANTIOXIDANT PROPERTIES OF THE METHANOL EXTRACT FROM THE LEAVES OF Solanum americanum.

Nwabiani¹,

Ogbuagu²,

Okerulu³

2023

JCSN

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“…Maltosa merupakan salah satu gula pereduksi sehingga akan bereaksi dengan asam dinitro salisilat (DNS). (Pratiwi et al, 2018). Perubahan warna diukur menggunakan spektrofotometer UV-Vis.…”

Section: Metodeunclassified

Pendekatan Metode Mikroenkapsulasi Enzim β-amilase pada Alginat untuk Reaksi Hidrolisis Pati Menjadi Maltosa

Maulia

Amran

2022

WARTA AKAB

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β-amilase merupakan enzim karbohidrolase yang menghidrolisis ikatan α-1,4-glikosidik pada pati secara spesifik menghasilkan maltosa. Penggunaan enzim β-amilase untuk menghasilkan maltosa selama ini terkendala oleh sulitnya pemisahan antara enzim dengan substrat sehingga hanya dapat digunakan untuk satu kali reaksi. Pada penelitian ini dilakukan pendekatan metode amobilisasi dengan cara mikroenkapsulasi β-amilase menggunakan Na-alginat dan glutaraldehid. Pembuatan kapsul alginat dilakukan dengan meneteskan campuran enzim, glutaraldehid dan alginat yang telah dilarutkan dalam buffer asetat 0,05 M pH 5,5 ke dalam larutan Ca2+ menggunakan syringe berdiameter 0,6 mm. Kadar alginat divariasikan dengan mengukur kinerja enzim pada penggunaan alginat 2% dan 3% dengan kadar kalsium yang dibuat tetap. Kadar kalsium divariasikan 2%, 3% dan 4% pada kadar alginat yang memberikan kinerja optimum. Aktivitas enzim β-amilase terenkapsulasi alginat dievauasi dengan mengukur jumlah maltosa yang dihasilkan. Jumlah maltosa ditentukan dengan metode spektrofotometri UV-Vis pada panjang gelombang 540 nm dengan pereaksi asam 3,5-dinitrosalisilat (DNS) sebagai pembentuk warna. Enzim β-amilase terenkapsulasi alginat memiliki aktivitas tertinggi sebesar 2,34 mg maltosa/50 butir kapsul/10 menit dengan kadar alginat 2% dan kalsium 3%.

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“…Prinsip dari metode dinitrosalisilat (DNS) adalah gugus aldehid pada rantai polisakarida dioksidasi menjadi gugus karboksil, disaat yang bersamaan gugus aldehid gula akan mereduksi asam 3,5dinitrosalisilat menjadi asam 3-amino-5-nitrosalisilat, Reaksi akan berlangsung secara simultan selama terdapat gula pereduksi dalam larutan yang akan diujikan (Hasanah & Iwan, 2015). Gula pereduksi pada sampel akan bereaksi dengan larutan DNS yang awalnya berwarna kuning akan menjadi warna jingga kemerahan atau membentuk senyawa asam 3-amino-5-nitrosalisilat berwarna kuning kecoklatan (Pratiwi et al, 2018). Satu unit enzim adalah jumlah enzim yang dibutuhkan untuk memecah 1 μmol selulosa menjadi gula pereduksi per menit pada kondisi pengujian Kode isolat Label Kultur…”

Section: Pendahuluanunclassified

SELECTION OF ISOLATES OF LOCAL ENDOPHYTIC BACTERIA CELLULOLYTIC STRAINS FROM MANGROVE ROOTS Ceriops tagal (Perr) C. B. Rob

Devy¹

2022

Photon

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Cellulose agricultural waste is a source of environmental pollution and an alternative solution to overcome it is to degrade this waste with the help of cellulolytic enzymes. The Biochemistry Laboratory of FMIPA Riau University has a number of endophytic bacterial isolates from the roots of Mangrove Ceriops tagal (Perr) C. B. Rob in the Bengkalis area whose potential to produce cellulolytic enzymes is not yet known. Qualitative selection in carboxy methyl cellulose (CMC) solid media from 24 isolates of endophytic bacteria that were able to live because they produced cellulolytic enzymes, only 22 isolates. Quantitatively using the DNS (Dinitro Salicylic Acid) method, 3 of these 22 isolates had relatively higher activity than other isolates. The activity of isolate C-9 (LBKURCC332) was 572x10-4±0.0069U/mL, isolate C-23(LBKURCC346) was 423x10-4±0.0027U/mL and isolate C-30 (LBKURCC353) was 384x10-4± 0.0003 U/mL and statistically these 3 cellulolytic activities were significantly different

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Perbandingan Metode Uji Gula Pereduksi Dalam Penentuan Aktivitas ?-L-Arabinofuranosidase Dengan Substrat Janur Kelapa (Cocos Nucifera)

Cited by 9 publications

References 3 publications

QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND THE ANTIOXIDANT PROPERTIES OF THE METHANOL EXTRACT FROM THE LEAVES OF Solanum americanum.

QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND THE ANTIOXIDANT PROPERTIES OF THE METHANOL EXTRACT FROM THE LEAVES OF Solanum americanum.

Pendekatan Metode Mikroenkapsulasi Enzim β-amilase pada Alginat untuk Reaksi Hidrolisis Pati Menjadi Maltosa

SELECTION OF ISOLATES OF LOCAL ENDOPHYTIC BACTERIA CELLULOLYTIC STRAINS FROM MANGROVE ROOTS Ceriops tagal (Perr) C. B. Rob

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