Per-follicle measurements indicate that anti-Müllerian hormone secretion is modulated by the extent of follicular development and luteinization and may reflect qualitatively the ovarian follicular status

Fanchin, Rénato; Louafi, Nabil; Lozano, Daniel H Méndez; Achour-Frydman, Nelly; Taieb, Julien; Frydman, R

doi:10.1016/j.fertnstert.2004.07.818

Cited by 8 publications

(15 citation statements)

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“…Within small follicles, moreover, AMH is expressed exclusively by granulosa cells with mitotic activity (22, 23), presumably because it interacts with mitogenic growth factors during follicle development (i.e., epidermal growth factor, transforming growth factor-β, and insulin-like growth factor-I) (24, 25). Therefore, our findings of a positive relationship between intrafollicular AMH concentrations and the ratios of E 2 /FSH and E 2 /androgens in the same follicles may represent the degree of granulosa cell proliferation during FSH-stimulated follicle growth, which could counteract AMH inhibition of FSH-dependent aromatase activity during folliculogenesis (6, 8, 20, 21). An alternate explanation for our findings is that decreased AMH bioactivity from polymorphisms in AMH or the AMH type II receptor might simultaneously increase AMH production and decrease AMH inhibition of follicle sensitivity to FSH (26).…”

Section: Discussionmentioning

confidence: 81%

“…At oocyte retrieval, follicular fluid was aspirated from the first follicle of each ovary, which was selected by accessibility and size of at least 16 mm in mean diameter, as calculated by 3 independent follicular measurements in perpendicular planes. These measurements were used to calculate the size of the follicle in which AMH predicts enhanced oocyte quality (6), while also controlling for follicle size as a confounding variable (8). …”

Section: Methodsmentioning

confidence: 99%

“…Following gonadotropin therapy for IVF-ET, AMH concentrations in small (8–12 mm in diameter) and large (16–20 mm in diameter) antral follicles positively correlate with granulosa cell responsiveness to FSH and negatively correlate with intrafollicular P levels (8). Moreover, AMH levels in these large antral follicles predict enhanced embryo implantation and successful pregnancy outcome, suggesting a role for AMH in oocyte development (6).…”

Section: Introductionmentioning

confidence: 99%

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Intrafollicular antimüllerian hormone levels predict follicle responsiveness to follicle-stimulating hormone (FSH) in normoandrogenic ovulatory women undergoing gonadotropin releasing-hormone analog/recombinant human FSH therapy for in vitro fertilization and embryo transfer

Dumesic

Lesnick

Stassart³

et al. 2009

Fertility and Sterility

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Objective-To investigate the relationship between antimullerian hormone (AMH) and steroidogenesis in follicles of normoandrogenic ovulatory women undergoing FSH therapy for IVF-ET. Design-Prospective cohort.Setting-Institutional/private practice.Patients-26 normoandrogenic ovulatory women. All women received GnRH analog and ovarian stimulation for IVF-ET.Interventions-Follicle fluid was aspirated at oocyte retrieval from the first follicle of each ovary.Main Outcome Measures-Follicle fluid was assayed for AMH, E 2 , P, androstenedione, T, dihydrotestosterone, insulin and FSH.Results-Intrafollicular AMH levels positively and negatively correlated with E 2 and FSH concentrations in follicles, respectively, causing a positive relationship between follicle fluid AMH levels and E2/FSH ratios as a measure of follicle sensitivity to FSH. A positive relationship also existed in follicles between AMH levels and E 2 /androgen ratios as a marker of aromatase activity. Conclusions-AMH

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Section: Discussionmentioning

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intrafollicular antimüllerian hormone levels predict follicle responsiveness to follicle-stimulating hormone (FSH) in normoandrogenic ovulatory women undergoing gonadotropin releasing-hormone analog/recombinant human FSH therapy for in vitro fertilization and embryo transfer

Dumesic

Lesnick

Stassart³

et al. 2009

Fertility and Sterility

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“…It has been demonstrated that AMH expression increases significantly from the primary through the early antral stages of folliculogenesis [13,57] and declines during the final follicular maturation process, suggesting a negative effect of follicle luteinization on the production of AMH in GCs [3,22,23]. Moreover, it has been shown a negative relationship between human FF AMH and P 4 at the time of egg retrieval, suggesting an inverse correlation between a more advanced process of luteinization and ability of the follicle to secrete AMH [20].…”

Section: Discussionmentioning

confidence: 99%

Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

Barberi

Ermini

Morelli

et al. 2012

J Assist Reprod Genet

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Purpose Down-regulation with gonadodropin-releasing agonist (GnRH-a) protocol during IVF stimulation leads to a severe endogenous LH suppression, which may affect the follicular development. The aim of the study was to evaluate the effects of recombinant LH (r-LH) administration, during late follicular development stages, in recombinant FSH (r-FSH) stimulated cycles on follicular fluid (FF) parameters and on cumulus cell quality. Methods Twenty patients undergoing IVF were stimulated in a long GnRH agonist protocol with r-FSH alone or with r-LH supplementation when the leading follicle reached diameter of 14 mm. FF was collected at the time of oocyte retrieval from 32 follicles ≥ 18 mm. Serum FSH, LH, estradiol (E 2 ), and progesterone (P 4 ) were evaluated on the day of hCG administration. Intra-follicular E 2 , P 4 , AMH and TGF-β were assayed. Total RNA from 18 individual cumuli was isolated for gene expression analyses. Results R-LH increased FF P 4 levels. FF TGF-β levels and PTGS2 and HAS2 expression in cumulus cells (CCs) positively correlated with increased P 4 levels observed in FFs, while a negative correlation was found between P 4 and AMH levels. Conclusions FF positive correlation between P 4 and TGF-β levels and CC expression of PTGS2 and HAS2 suggest an association with a better follicle quality. In addition, our data suggest that late follicular phase r-LH supplementation leads to a more advanced stage of follicular maturation.

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“…Serum E 2 , FSH, LH, T, and A concentrations were determined by using enzyme-amplified time-resolved fluorescence technology (DPC 1000 automatic analyzer, Los Angeles, CA, American). To make allowance for the variation in FF volume, FF hormone levels were adjusted to the relative content of per gram protein [Fanchin et al 2005;Franchimont et al 1989]. FF protein contents were determined by using routine biuret reaction method (BCA protein assay reagent kit, Pierce Rockford, IL, American).…”

Section: Hormone Assaysmentioning

confidence: 99%

The relationship between serum anti-Müllerian hormone levels and the follicular arrest for women with polycystic ovary syndrome

Hua

et al. 2014

Systems Biology in Reproductive Medicine

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(2015) The relationship between serum anti-Müllerian hormone levels and the follicular arrest for women with polycystic ovary syndrome, Systems Biology in Reproductive Medicine, 61:2, 103-109, DOI: 10.3109/19396368.2014.973123 To link to this article: https://doi.org/10. 3109/19396368.2014 AbstractThe aim of this study was to ascertain whether higher levels of serum anti-Mü llerian hormone (AMH) are associated with the ovarian follicular arrest in women with polycystic ovary syndrome (PCOS). This prospective study compared AMH levels between serum and dominant follicular fluid (FF) in ovulatory polycystic ovary (PCO) women and anovulatory (menstrual cycle !60 days.) PCOS women. All 102 women provided a baseline hormone profile and underwent controlled ovarian hyperstimulation (COH). The anovulatory PCO women had a similar body mass index (BMI), antral follicle count (AFC), and baseline serum AMH levels as the ovulatory PCO women except that their median luteinizing hormone (LH; 10.0 mIU/ml), testosterone (T) (0.61 ng/l), and androstenedione (A) (3.47 ng/l) levels were significantly higher than ovulatory PCO women (4.9 mIU/m; 0.43 ng/l and 2.09 ng/l, respectively). The ovarian response to gonadotropin stimulation during COH including serum AMH on the day of HCG administration and dominant FF AMH at 36 hours after HCG administration, total follicle stimulating hormone (FSH) dose administrated, peak E 2 , (estrogen) levels and number of occytes retrieved were all similar between women with anovulatory and ovulatory PCO. Using multiple regression analysis it was found that an important independent determinant affecting AMH was AFC, as opposed to LH and T. Logistic regression analysis showed that the two most important factors affecting ovulation were serum LH and T, whereas serum AMH and AFC were not selected for inclusion in the model. The reduction in AMH during COH occurs as a consequence of dominant follicles with a corresponding reduction in small antral follicle number. Elevated serum AMH levels in PCO women seem to be related only to follicular excess and not follicular arrest.

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Per-follicle measurements indicate that anti-Müllerian hormone secretion is modulated by the extent of follicular development and luteinization and may reflect qualitatively the ovarian follicular status

Cited by 8 publications

References 0 publications

Intrafollicular antimüllerian hormone levels predict follicle responsiveness to follicle-stimulating hormone (FSH) in normoandrogenic ovulatory women undergoing gonadotropin releasing-hormone analog/recombinant human FSH therapy for in vitro fertilization and embryo transfer

Intrafollicular antimüllerian hormone levels predict follicle responsiveness to follicle-stimulating hormone (FSH) in normoandrogenic ovulatory women undergoing gonadotropin releasing-hormone analog/recombinant human FSH therapy for in vitro fertilization and embryo transfer

Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

The relationship between serum anti-Müllerian hormone levels and the follicular arrest for women with polycystic ovary syndrome

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