Peptoniphilus faecalis sp. nov., isolated from swine faeces

Abstract

An obligately anaerobic, Gram-positive, non-motile, coccus-shaped bacterial strain designated AGMB00490T was isolated from swine faeces. 16S rRNA gene sequence-based phylogenetic analysis indicated that the isolate belongs to the genus Peptoniphilus and that the most closely related species is Peptoniphilus gorbachii WAL 10418T (=KCTC 5947T, 97.22 % 16S rRNA gene sequence similarity). Whole genome sequence analysis determined that the DNA G+C content of strain AGMB00490T was 31.2 mol% and moreover that the genome size and numbers of tRNA and rRNA genes were 2 129 517 bp, 34 and 10, respectively. Strain AGMB00490T was negative for oxidase and urease; positive for catalase, indole production, arginine arylamidase, leucine arylamidase, tyrosine arylamidase and histidine arylamidase; and weakly positive for phenylalanine arylamidase and glycine arylamidase. The major cellular fatty acids (>10 %) of the isolate were determined to be C16 : 0 and C18 : 1 ω9c. Strain AGMB00490T produced acetic acid as a major end product of metabolism. Accordingly, phylogenetic, physiologic and chemotaxonomic analyses revealed that strain AGMB00490T represents a novel species for which the name Peptoniphilus faecalis sp. nov. is proposed. The type strain is AGMB00490T (=KCTC 15944T=NBRC 114159T).

Peptoniphilus coli sp. nov. and Peptoniphilus urinae sp. nov., isolated from humans

“…Peptoniphilus spp. have mostly been isolated from human clinical samples (Ezaki et al 2001 ; Citron et al 2012 ; Diop et al 2019 ) and in some rare cases they are isolated from animals like swine, cattle or pig (Rooney et al 2011 ; Wylensek et al 2020 ; Ryu et al 2021 ). Currently, there are more than 20 species validly published with standing in the nomenclature ( https://lpsn.dsmz.de/genus/peptoniphilus ).…”

Phytogenic supplement containing menthol, carvacrol and carvone ameliorates gut microbiota and production performance of commercial layers

“…ASVs assigned to Fastidiosipila by SILVA, are among those assigned to Clostridium genus in Green Genes database. Another relatively recently annotated genus Peptoniphilus 23 are common genital microbiota in humans 26 and also very recently reported in swine feces 27 . Among the genera reported as suppressed by phytogen supplementation, we have a few generally accepted beneficial genera such as Lactobacillus, Ruminococcus and Butyricicoccus and a wide range of genera with known and emerging pathogenic behaviours.…”

Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse

“…(). C 16 : 0 , one of the major fatty acids of CBA3646 T , has also been shown to be the major fatty acid of strains of members of the genus Peptoniphilus [13–18], indicating that CBA3646 T shares cellular properties with members of the genus Peptoniphilus . C 14 : 0 was a major fatty acid in P.…”

“…The phenotypic characteristics of CBA3646 T and those of the reference strains P. asaccharolyticus KCTC 3321 3). C 16 : 0 , one of the major fatty acids of CBA3646 T , has also been shown to be the major fatty acid of strains of members of the genus Peptoniphilus [13][14][15][16][17][18], indicating that CBA3646 T shares cellular properties with members of the genus Peptoniphilus. C 14 : 0 was a major fatty acid in P. asaccharolyticus KCTC 3321 T but was a minor fatty acid in CBA3646 T .…”

“…its species were formerly classified as members of the genus Peptostreptococcus) in 2001 on the basis of the sequence of the 16S rRNA gene [8,12]. Species of the genus Peptoniphilus have been successfully isolated from various human clinical specimens [13][14][15][16][17] and swine faeces [18,19]. Bacteria of the genus Peptoniphilus are Gram-stain-positive, non-motile, anaerobic cocci.…”