Peptidomic Analysis on Mouse Lung Tissue Reveals AGDP as a Potential Bioactive Peptide against Pseudorabies Virus Infection

Abstract

Pseudorabies virus (PRV) infection could cause severe histopathological damage via releasing multiple factors, including cytokines, peptides, etc. Here, peptidomic results showed that 129 peptides were identified in PRV-infected mouse lungs and were highly involved in the process of PRV infection. The role of one down-regulated biological peptide (designated as AGDP) during PRV infection was investigated. To verify the expression profiles of AGDP in response to PRV infection, the expression level of the precursor protein of AGDP mRNA was significantly decreased in PRV-infected mouse lungs and cells. The synthesized AGDP-treating cells were less susceptible to PRV challenges than the controls, as demonstrated by the decreased virus production and gE expression. AGDP not only inhibited the expression of TNF-α and IL-8 but also appeared to suppress the extracellular release of high-mobility group box 1 (HMGB1) by inhibiting the output of nuclear HMGB1 in cells. AGDP could also inhibit the degradation of IκBα and the phosphorylation levels of P65 after PRV infection. In total, our results revealed many meaningful peptides involved in PRV infection, thereby enhancing the current understanding of the host response to PRV infection, and how AGDP may serve as a promising candidate for developing novel anti-PRV drugs.

Peptidome analysis reveals critical roles for peptides in a rat model of intestinal ischemia/reperfusion injury

“…Recent advances in peptidomics, which is an emerging field derived from proteomics are actively being used to study multiple diseases, such as bronchopulmonary dysplasia, acute myocardial infarction, and neonatal respiratory distress syndrome [9,40,41]. Some of the advantages of peptide treatment are low molecular weight, low toxicity, and specific targeting, and this makes it a promising new regime that could be applied to a wide range of diseases [29,[42][43][44][45]. However, supporting data from peptidomics studies of the intestine are currently unavailable.…”