Peptidomic Analysis of Cultured Cardiomyocytes Exposed to Acute Ischemic-Hypoxia

Wu, Linquan; Li, Hua; Li, Xing; Chen, Yumei; Zhang, Qi‐Jun; Cheng, Zijie; Fan, Yi; Qian, Lei; Song, Guixian

doi:10.1159/000456282

Cited by 19 publications

(17 citation statements)

References 54 publications

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“…Secondly, by analyzing the cellular component category, we found that the protein precursors are located throughout the subcellular regions, which indicates that these peptides exist in various cellular components. Moreover, the main cellular component of the identified peptides was associated with the membrane structure, which is consistent with the results of Wu et al [28]. .…”

Section: Discussionsupporting

confidence: 91%

“…Although FDR is more accurate than the p value for detecting differentially expressed peptides, we would get very few peptides though FDR. Because we found that some studies also applied the p value to peptidomic analysis [28, 47], we used p values in our study as well. There were many false positives in our study with this method and only the PDFV has been validated by cell experiments; the other peptides were reported purely on the basis of the p value and without any further validation.…”

Section: Discussionmentioning

confidence: 99%

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Peptidomic Analysis of Endometrial Tissue from Patients with Ovarian Endometriosis

Xue

et al. 2018

Cell Physiol Biochem

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Background/Aims: Ovarian endometriosis (OvE) is ovarian cyst that is lined with endometrial tissue. They are found in 17–44% of women with endometriosis. Their clinical manifestations include pelvic pain, dysmenorrhea, dyspareunia, and infertility. Although the incidence of OvE has increased yearly, the exact pathogenesis of OvE is still unclear. We used peptidomics, an emerging branch of proteomics, to identify differentially expressed peptides in order to determine the possible roles of these peptides in the pathogenesis of OvE. Methods: The ectopic and eutopic endometria of OvE were used to extract peptides with 10-kDa molecular weight cutoff filters, and the peptide precursor proteins were then identified with PEAKS software, followed by quantification with the TMT labeling method and subsequent analysis by liquid chromatography-tandem mass spectrometry. Gene ontology (GO) analysis, pathway analysis, SMART, and SABLE were used to study the possible functions of these peptide according to their precursor proteins’ function. The effects of peptides derived from VCAM-1 (PDFV) on endometrial stromal cell (ESC) migration and invasion were examined with wound healing assays and Transwell assays and the expression of E-cadherin was detected by western blotting. Results: A total of 491 peptides were identified with abundant differences between the two groups of samples (p < 0.05, and absolute fold change ≥ 2). SMART and SABLE database showed that 42 of the 491 peptides were located in the conserved structural domains of their protein precursors and contained secondary structure and, among them, 2 peptides’ precursor proteins were associated with the cell proliferation. Additionally, 5 peptides’ precursor proteins were associated with endometriosis. Our study confirmed that PDFV promoted ESC migration and invasion and reduced E-cadherin expression (p < 0.05). Conclusion: PDFV and its precursor protein VCAM-1 may be involved in the process of OvE formation by reducing the expression of E-cadherin. The peptidomics analysis provides new insight for future studies of the mechanisms of OvE development.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Peptidomic Analysis of Endometrial Tissue from Patients with Ovarian Endometriosis

Xue

et al. 2018

Cell Physiol Biochem

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“…Neonatal rat ventricular cardiomyocytes were extracted as previously described. 19 Briefly, for 2-3 days, neonatal rats were anesthetized with 75% ethanol and hearts were sheared and immediately placed in cold phosphate buffered saline (PBS, Wisent, CA). Hearts were then digested using 0.4% Type 2 collagenase/0.6% pancreatin (Sigma).…”

Section: Cell Culturementioning

confidence: 99%

Overexpression of lncRNA Gm2691 attenuates apoptosis and inflammatory response after myocardial infarction through PI3K/Akt signaling pathway

Tian

et al. 2019

IUBMB Life

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Acute myocardial infarction is one of the most threatening disease in the world. In previous studies, numerous dysregulated lncRNAs exposed to ischemic reperfusion injury have been identified. In this differential lncRNAs, Gm2691 attracted our attention due to its high fold change. The aim of the study was to investigate the function and mechanism of lncRNA Gm2691 in ischemic reperfusion injury. AnaeroPack anaerobic system treated neonatal rat ventricular cardiomyocytes were used to analyze the function of lncRNA Gm2691 in vitro. Tunel, Caspase3, and inflammation markers were detected to evaluate apoptosis and inflammatory response. Rat acute myocardial infarction was performed to elucidate the function of lncRNA Gm2691 in vivo. The results showed that LncRNA Gm2691 improved the cardiac function and attenuated the inflammatory response in vivo. We also found that lncRNA Gm2691 reduced the apoptosis and improved cell survival rates in anaeroPack anaerobic system treated neonatal rat ventricular cardiomyocytes. Western blot analysis revealed that lncRNA Gm2691 decreased Akt and ERK1/2 activities, suggesting that lncRNA Gm2691 may functioned through Akt signaling pathway. We verified the function and mechanism of lncRNA Gm2691 and provide evidence that lncRNA Gm2691 may play important role in ischemic reperfusion injury, and understanding the precise role of Gm2691 will undoubtedly shed new light on the clinical treatment.

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“…Segmental functional peptides were previously reported with biological functions associated with precursor proteins (Dumont & Bischoff, ; Gao et al, ; Jagot‐Lacoussiere et al, ; Kawaguchi et al, ). We identified protein precursors of differential expressed peptides associated with PCOS through the genome‐wide Open Targets Platform (Wu et al, ). Most precursor proteins were associated with obesity (data not shown), which indicated that differentially secreted peptides were derived from adipose tissues.…”

Section: Discussionmentioning

confidence: 99%

Analysis of secreted peptidome from omental adipose tissue in polycystic ovarian syndrome patients

Jia

Tao

Xue

et al. 2018

Journal Cellular Physiology

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Polycystic ovarian syndrome (PCOS) is a common endocrinopathy associated with increased risk of metabolic disorders. Prevalence of adiposity and obesity is greater in women suffering from PCOS. Moreover, adipose tissue dysfunction has been demonstrated in PCOS patients, particularly in abdominal adipose tissue. This dysfunction likely aggravates the metabolic and reproductive abnormalities. We used liquid chromatography-mass spectrometry to compare the peptides secreted from PCOS and non-PCOS abdominal adipose tissue. We detected 298 upregulated peptides and 31 downregulated peptides (absolute fold change ≥ 2 and p < 0.05). Twenty-nine peptides were only detected in the PCOS group, while 18 were only detected in the control group. In addition, we demonstrate that these cleavage products are not degradation products of the proteasome based on previous studies reported. Gene Ontology enrichment and pathway analysis were performed to study differentially secreted peptides through their precursor proteins. We identified 12 peptides from 10 precursor proteins associated with PCOS, and 6 peptide sequences were located in the functional domains of their corresponding precursor proteins. These results provide a deeper understanding of adipose tissue-derived peptides in PCOS for future functional studies.

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Peptidomic Analysis of Cultured Cardiomyocytes Exposed to Acute Ischemic-Hypoxia

Cited by 19 publications

References 54 publications

Peptidomic Analysis of Endometrial Tissue from Patients with Ovarian Endometriosis

Peptidomic Analysis of Endometrial Tissue from Patients with Ovarian Endometriosis

Overexpression of lncRNA Gm2691 attenuates apoptosis and inflammatory response after myocardial infarction through PI3K/Akt signaling pathway

Analysis of secreted peptidome from omental adipose tissue in polycystic ovarian syndrome patients

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