Peptides derived from the C-terminal domain of HIV-1 Viral Protein R in lipid bilayers: Structure, membrane positioning and gene delivery

Marquette, Arnaud; Leborgne, Christian; Schartner, Vanessa; Salnikov, Evgeniy S.; Bechinger, Burkhard; Kichler, Antoine

doi:10.1016/j.bbamem.2019.183149

Cited by 16 publications

(9 citation statements)

References 53 publications

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“…Altogether, these studies suggested that viral glycoproteins might target raft domains within the mammalian plasma membrane (Figure 3b). In addition, negatively charged phospholipids, such as PS, can also affect the adsorption and the membrane location of fusion peptides, as shown in a recent study involving POPC/POPS vesicles and peptides derived from the C-terminal domain of HIV-1 viral protein R [114]. Glycoproteins of other enveloped viruses such as influenza or SARS-CoV viruses were also shown to have a strong association with raft-like lipid domains [54,115].…”

Section: Protein-lipid Interactionsmentioning

confidence: 95%

Mimicking the Mammalian Plasma Membrane: An Overview of Lipid Membrane Models for Biophysical Studies

Luchini

Vitiello

2020

Biomimetics

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Cell membranes are very complex biological systems including a large variety of lipids and proteins. Therefore, they are difficult to extract and directly investigate with biophysical methods. For many decades, the characterization of simpler biomimetic lipid membranes, which contain only a few lipid species, provided important physico-chemical information on the most abundant lipid species in cell membranes. These studies described physical and chemical properties that are most likely similar to those of real cell membranes. Indeed, biomimetic lipid membranes can be easily prepared in the lab and are compatible with multiple biophysical techniques. Lipid phase transitions, the bilayer structure, the impact of cholesterol on the structure and dynamics of lipid bilayers, and the selective recognition of target lipids by proteins, peptides, and drugs are all examples of the detailed information about cell membranes obtained by the investigation of biomimetic lipid membranes. This review focuses specifically on the advances that were achieved during the last decade in the field of biomimetic lipid membranes mimicking the mammalian plasma membrane. In particular, we provide a description of the most common types of lipid membrane models used for biophysical characterization, i.e., lipid membranes in solution and on surfaces, as well as recent examples of their applications for the investigation of protein-lipid and drug-lipid interactions. Altogether, promising directions for future developments of biomimetic lipid membranes are the further implementation of natural lipid mixtures for the development of more biologically relevant lipid membranes, as well as the development of sample preparation protocols that enable the incorporation of membrane proteins in the biomimetic lipid membranes.

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Section: Protein-lipid Interactionsmentioning

confidence: 95%

Mimicking the Mammalian Plasma Membrane: An Overview of Lipid Membrane Models for Biophysical Studies

Luchini

Vitiello

2020

Biomimetics

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“…5c). It has been shown that mHTT has high affinity for bioengineered lipid membranes and that insertion of these proteins into these membranes triggers their aggregation 47 . Our analysis also revealed that the largest HTTEx1Q72-mCherry puncta (volume > 5 µm 3 ) were nearly all (90-100%) localized at the myotube surface at DCC 7-21 (Fig.…”

Section: Modulating Neuronal Activity Alters Nm Transmission Of Mhttex1mentioning

confidence: 99%

Transmission-selective muscle pathology induced by active propagation of mutant huntingtin across the human neuromuscular synapse

Dinamarca¹,

Colombo

Brykczynska

et al. 2021

Preprint

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A potential explanation for the spatiotemporal accumulation of pathological lesions in the brain of patients with neurodegenerative protein misfolding diseases (PMDs) is cell-to-cell transmission of aggregation-prone, misfolded proteins. Little is known about central to peripheral transmission and its contribution to pathology. We show that transmission of Huntington’s disease- (HD-) associated mutant HTT exon 1 (mHTTEx1) occurs across the neuromuscular junctions in human iPSC cultures and in vivo in wild-type mice. We found that transmission is an active and dynamic process, that happens prior to aggregate formation and is regulated by synaptic activity. Furthermore, we find that transmitted mHTTEx1 causes HD-relevant pathology at a molecular and functional level in human muscle cells, even in the presence of ubiquitous expression mHTTEx1. With this work we uncover a casual-link between mHTTEx1 synaptic transmission and pathology, highlighting the therapeutic potential in blocking toxic protein transmission in PMDs.

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“…The interactions of the peptides with the bilayer interface eventually result in pore-formation [191] and membrane lysis [192], thereby modulating nucleic acid delivery. Inspired by the ability to enhance the efficiency of several gene delivery systems, researchers in the past extensively used LAH4 peptides as targeting moiety [187,[193][194][195][196][197][198]. By now, there is a growing number of peptidic, as well as polymeric and lipidic carriers that utilize other histidine-rich moieties, such as O 10 H 6 [199], MS(O 10 H 6 ) [200,201], histidine-rich Tat peptide [202][203][204], or His6 RPCs [205,206] to develop new promising gene delivery strategies.…”

Section: Histidine-rich Peptidesmentioning

confidence: 99%

Peptide-Assisted Nucleic Acid Delivery Systems on the Rise

Tarvirdipour

Skowicki

Schoenenberger

et al. 2021

IJMS

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Concerns associated with nanocarriers’ therapeutic efficacy and side effects have led to the development of strategies to advance them into targeted and responsive delivery systems. Owing to their bioactivity and biocompatibility, peptides play a key role in these strategies and, thus, have been extensively studied in nanomedicine. Peptide-based nanocarriers, in particular, have burgeoned with advances in purely peptidic structures and in combinations of peptides, both native and modified, with polymers, lipids, and inorganic nanoparticles. In this review, we summarize advances on peptides promoting gene delivery systems. The efficacy of nucleic acid therapies largely depends on cell internalization and the delivery to subcellular organelles. Hence, the review focuses on nanocarriers where peptides are pivotal in ferrying nucleic acids to their site of action, with a special emphasis on peptides that assist anionic, water-soluble nucleic acids in crossing the membrane barriers they encounter on their way to efficient function. In a second part, we address how peptides advance nanoassembly delivery tools, such that they navigate delivery barriers and release their nucleic acid cargo at specific sites in a controlled fashion.

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Peptides derived from the C-terminal domain of HIV-1 Viral Protein R in lipid bilayers: Structure, membrane positioning and gene delivery

Cited by 16 publications

References 53 publications

Mimicking the Mammalian Plasma Membrane: An Overview of Lipid Membrane Models for Biophysical Studies

Mimicking the Mammalian Plasma Membrane: An Overview of Lipid Membrane Models for Biophysical Studies

Transmission-selective muscle pathology induced by active propagation of mutant huntingtin across the human neuromuscular synapse

Peptide-Assisted Nucleic Acid Delivery Systems on the Rise

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