Peptides and vasomotor mechanisms

Mione, Marina; Ralevic, Vera; Burnstock, Geoffrey

doi:10.1016/0163-7258(90)90027-y

Cited by 84 publications

(37 citation statements)

References 374 publications

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“…Furthermore, our data indicate desensitization upon continued exposure to SP. These findings are consistent with previous observations in other vascular beds (Zawadzki et al 1981, Furchgot 1984, Mione et al 1990.…”

Section: Discussionsupporting

confidence: 83%

“…CGRP induced concentration-dependent vasorelaxation in agreement with findings in the in situ in vivo perfused tibia model (Lindblad et al 1995) as well as other vascular beds (Brain et al 1985, Edvinsson et al 1989, Mione et al 1990, Prieto et al 1991. In the present study, we found that only a small part of the CGRP-induced relaxation was endothelium-mediated, since relaxation by CGRP was pronounced after removal of the endothelium.…”

Section: Discussionsupporting

confidence: 81%

See 1 more Smart Citation

Vasorelaxation in isolated bone arteries: Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide bradykinin studied in pigs

Lundgaard¹,

Aalkjær²,

Bjurholm

et al. 1997

Acta Orthopaedica Scandinavica

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 81%

Vasorelaxation in isolated bone arteries: Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide bradykinin studied in pigs

Lundgaard¹,

Aalkjær²,

Bjurholm

et al. 1997

Acta Orthopaedica Scandinavica

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“…Trans-mural complexes, composed of those sensory nerve fibres releasing calcitonin gene-related peptide (CGRP), substance P (SP), vasoactive intestinal peptide (VIP), nitric oxide (NO) and adenosine 5 0 -triphosphate (ATP) (Lundberg, 1996), act in most cases as modulators of the autonomic sympathetic and parasympathetic activity in vessels of the intestinal tract (Maggi and Meli, 1988;Mione et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…Calcitonin gene-related peptide is a 37-amino-acid vasoactive neurotransmitter that has been shown to act as a physiological antagonist of autonomic nerve-dependent increases in vascular tone (Mione et al, 1990). For example, CGRP is known as the main neuropeptide involved in the capsaicin-sensitive nerve-induced vasodilatation of the rat mesenteric vascular bed (Kawasaki et al, 1988(Kawasaki et al, , 1990.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the non‐adrenergic/non‐cholinergic response to perivascular nerve stimulation in the double‐perfused mesenteric bed of the mouse

Legros

Tirapelli

Brochu

et al. 2007

British J Pharmacology

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Background and purpose: Calcitonin gene-related peptide (CGRP), a capsaicin-sensitive neuromodulator of splanchnic vascular tone in several animal species, remains poorly investigated in mouse models. We therefore assessed whether endogenous CGRP is a non-adrenergic/non-cholinergic (NANC) neuromodulator in the mesenteric vascular bed of the mouse. Experimental approach: Arterial and venous changes in perfusion pressure in response to perivascular nerve stimulation (PNS) were monitored in the mouse mesenteric bed under basal conditions or precontracted with KCl (artery) or U46619 (vein) in circuits pretreated with guanethidine, atropine, indomethacin and prazosin. Arterial responses to NANC were also characterized with a CGRP1 antagonist, haCGRP 8À37. Finally, the PNS-induced release of arterial CGRP was measured by enzyme immunoassay. Key results: HaCGRP 8À37 enhanced PNS-induced arterial increases in perfusion pressure under basal tone. PNS-induced stimulation of NANC triggered an haCGRP 8À37 or capsaicin-sensitive reduction in perfusion pressure of the pre-contracted arterial bed only. Chemical removal of the endothelium inhibited PNS-and haCGRP-induced reduction in perfusion pressure in the arterial mesenteric bed. Responses to NANC nerves were reduced by guanylate and adenylate cyclase inhibitors (1H-[1,2,4]oxadiazole[4,3-a] quinoxalin-1-one (ODQ)) and [9-(tetrahydro-2-furanyl)-9H-purin-6-amine] (SQ 22 536), respectively. A neuronal NOS inhibitor (7-nitroindazole; 7-NI) also enhanced the response to NANC in vessels from wild-type, eNOS KO but not iNOS KO mice. Finally, PNS enhanced the release of immunoreactive CGRP from the perfused arterial mesenteric bed. Conclusions and implications: Our study demonstrates a role for CGRP in the NANC-dependent reduction in perfusion pressure of the arterial but not venous mesenteric bed of the mouse. (2007) Keywords: CGRP; haCGRP 8À37 ; neuronal nitric oxide synthase; arterial mesenteric vasculature; mouse model British Journal of Pharmacology

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“…In salt-sensitive hypertension, synthesis and release of vasoactive neuropeptides from sensory ganglia innervating the splanchnic circulation are increased, 10,44 and this may play a role in blood pressure regulation, 45 but it is not known whether these are related to the observed decreases in the activity of NADPH oxidase.…”

Section: Discussionmentioning

confidence: 99%

Differential Regulation of NADPH Oxidase in Sympathetic and Sensory Ganglia in Deoxycorticosterone Acetate–Salt Hypertension

et al. 2007

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Abstract-We demonstrated recently that superoxide anion levels are elevated in prevertebral sympathetic ganglia of deoxycorticosterone acetate-salt hypertensive rats and that this superoxide anion is generated by reduced nicotinamideadenine dinucleotide phosphate oxidase. In this study we compared the reduced nicotinamide-adenine dinucleotide phosphate oxidase enzyme system of dorsal root ganglion (DRG) and sympathetic celiac ganglion (CG) and its regulation in hypertension. The reduced nicotinamide-adenine dinucleotide phosphate oxidase activity of ganglion extracts was measured using fluorescence spectrometry of dihydroethidine; the activity in hypertensive dorsal root ganglion was 34% lower than in normotensive DRG. In contrast, activity was 79% higher in hypertensive CG than normotensive CG. mRNA for the oxidase subunits NOX1, NOX2, NOX4, p47 phox , and p22 phox were present in both CG and DRG; mRNA for NOX4 was significantly higher in CG than in DRG. The levels of mRNA and protein expression of the membrane-bound catalytic subunit p22 phox and of the regulatory subunits p47 phox and Rac-1 were measured in CG and DRG in normotensive and hypertensive rats. p22 phox mRNA and protein expression was greater in CG of hypertensive rats but not in DRG. Compared with normotensive controls, p47 phox mRNA and protein, as well as Rac-1 protein, were significantly decreased in hypertensive DRG but not in CG. Immunohistochemical staining of p47 phox showed translocation from cytoplasm to membrane in hypertensive CG but not in hypertensive DRG. This suggests that reduced nicotinamide-adenine dinucleotide phosphate oxidase activation in sympathetic neurons and sensory neurons is regulated in opposite directions in hypertension. This differential regulation may contribute to unbalanced vasomotor control and enhanced vasoconstriction in the splanchnic circulation. Key Words: superoxide Ⅲ sympathetic ganglia Ⅲ sensory ganglia Ⅲ NADPH oxidase Ⅲ rat Ⅲ hypertension T he splanchnic circulation is of great importance in regulating systemic blood pressure. It receives Ϸ60% of the cardiac output and contains approximately one third of the total blood volume. 1 The splanchnic circulation is innervated by both the sympathetic division of the autonomic nervous system (prevertebral sympathetic ganglion neurons, including celiac ganglia [CGs] and superior and inferior mesenteric ganglia) and by spinal sensory nerves (dorsal root ganglia neurons [DRGs]). Elevated sympathetic nervous system activation has been shown in various types of hypertension. 2,3 In particular, sympathetic ganglionic blockade can reduce the arterial blood pressure increase in deoxycorticosterone acetate (DOCA)-salt hypertension, 4 indicating an important role of sympathetic ganglia in the development and maintenance of salt-induced hypertension. On the other hand, sensory nerves play a counterregulatory role in preventing increases in blood pressure through either afferent baroreceptormediated mechanisms 5 or efferent release of vasodilatory neuropeptides, such as ...

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Peptides and vasomotor mechanisms

Cited by 84 publications

References 374 publications

Vasorelaxation in isolated bone arteries: Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide bradykinin studied in pigs

Vasorelaxation in isolated bone arteries: Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide bradykinin studied in pigs

Characterization of the non‐adrenergic/non‐cholinergic response to perivascular nerve stimulation in the double‐perfused mesenteric bed of the mouse

Differential Regulation of NADPH Oxidase in Sympathetic and Sensory Ganglia in Deoxycorticosterone Acetate–Salt Hypertension

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