“…The 4 epitopes, IGF-IR-6-26, IGF-IR-26-42, IGF-IR-56-81, and IGF-IR-234-252, so named according to their epitope span,were chemically synthesized and characterized, as previously described. 32 Based on initial characterization in vitro, we selected 2 of the peptide sequences, IGF-1R-56-81 and IGF-1R-234-252 for integration into an engineered recombinant peptide with a "promiscuous" Measles virus fusion (MVF) T-cell epitope (KLLSLIKGVIVHRLEGVE) with a 4-residue (GPSL) linker sequence. We subsequently examined the immunogenicity of these peptide mimics in rabbits and mice, as previously described.…”