Peptide transporter 1 (PepT1) in fish: A review

Wang, Junli; Yan, Xiao; Lu, Rongzhu; Meng, Xiaolin; Nie, Guoxing

doi:10.1016/j.aaf.2017.06.007

Cited by 33 publications

(17 citation statements)

References 81 publications

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“…A similar positive impact on growth was also observed with insect meal in the present study performed in rainbow trout. It is known that free amino acids and small peptides are absorbed specifically and more rapidly than the bolus of amino acids produced by protein digestion, especially in juveniles (Oliva-Teles et al 1999 ; Lundquist and Artursson 2016 ; Wang et al 2017 ). Furthermore, amino acids, especially branched-chain amino acids, are known as signaling molecules.…”

Section: Discussionmentioning

confidence: 99%

Characterizing alternative feeds for rainbow trout (O. mykiss) by 1H NMR metabolomics

et al. 2018

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IntroductionFish feed formulations are constantly evolving to improve the quality of diets for farmed fish and to ensure the sustainability of the aquaculture sector. Nowadays, insect, microalgae and yeast are feedstuff candidates for new feeds. However, the characterization of aquafeed is still based on proximate and targeted analyses which may not be sufficient to assess feed quality.ObjectivesOur aim was to highlight the soluble compounds that specifically differ between selected plant-based feeds complemented with alternative feedstuffs and discuss their origin and potential for fish nutrition.MethodsA growth trial was carried out to evaluate growth performances and feed conversion ratios of fish fed plant-based, commercial, insect, spirulina and yeast feeds. 1H NMR metabolomics profiling of each feed was performed using a CPMG sequence on polar extracts. Spectra were processed, and data were analyzed using multivariate and univariate analyses to compare alternative feeds to a plant-based feed.ResultsFish fed insect or yeast feed showed the best growth performances associated with the lowest feed conversion ratios compared to plant-based feed. Soluble compound 1H NMR profiles of insect and spirulina alternative feeds differed significantly from the plant-based one that clustered with yeast feed. In insect and spirulina feeds, specific differences compared to plant-based feed concerned glycerol and 3-hydroxybutyrate, respectively.ConclusionThis strategy based on compositional differences between plant-based and alternative feeds can be useful for detecting compounds unsuspected until now that could impact fish metabolism.Electronic supplementary materialThe online version of this article (10.1007/s11306-018-1454-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Characterizing alternative feeds for rainbow trout (O. mykiss) by 1H NMR metabolomics

et al. 2018

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“…Protein extracts and hydrolysates are useful dietary ingredients for fish because they supply free compounds that can act as feed attractant, balance the absorption kinetic of amino acids in complete feed, and may contain bioactive peptides [10][11][12][13]. Furthermore, teleosts can specifically benefit of the direct absorption of small peptides in the larval stage and in the later stages, especially through the PepT1 transporter, which is activated during refeeding after a fasting state to ensure compensatory growth as compared to mammals and birds [14,15].…”

Section: Introductionmentioning

confidence: 99%

Integrative Metabolomics for Assessing the Effect of Insect (Hermetia illucens) Protein Extract on Rainbow Trout Metabolism

Roques

Deborde

Guimas³

et al. 2020

Metabolites

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Nutrition of high trophic species in aquaculture is faced with the development of sustainable plant-based diets. Insects seem particularly promising for supplementing plant-based diets. However, the complex effect of whole insect meal on fish metabolism is not well understood, and even less is known about insect meal extracts. The purpose of this work was to decipher the metabolic utilization of a plant-based diet supplemented with the gradual addition of an insect protein extract (insect hydrolysate at 0%, 5%, 10% and 15%). 1H-NMR profiling was used to assess metabolites in experimental diets and in fish plasma, liver and muscle. A significant dose-dependent increase in growth and feed efficiency with increasing insect extract amounts was observed. The incremental incorporation of the insect extract in diet had a significant and progressive impact on the profile of dietary soluble compounds and trout metabolome. The metabolites modulated by dietary insect extracts in plasma and tissues were involved in protein and energy metabolism. This was associated with the efficient metabolic use of dietary free amino acids toward protein synthesis through the concomitant supply of balanced free amino acids and energy substrates in muscle. The findings provide new insights into how the dietary food metabolome affects fish metabolism.

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“…The K m (1.07 mM) corresponds with those reported for dipeptides in numerous studies in fish. These range from ~0.1–10 mM as calculated via numerous techniques including brush border membrane vesicle uptake, everted sleeves and vector expression systems (reviewed in: Verri et al ., ; Wang et al ., ). A similar range in dipeptide K m values has also been reported in mammalian systems (Abe & Ohmama, ; Fei et al ., ; Ganapathy & Leibach, ).…”

Section: Discussionmentioning

confidence: 97%

Acquisition of alanyl‐alanine in an Agnathan: Characteristics of dipeptide transport across the hindgut of the Pacific hagfish Eptatretus stoutii

Weinrauch

Blewett

Glover

et al. 2019

Journal of Fish Biology

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This study used 3 H-L -alanyl-L -alanine to demonstrate dipeptide uptake using in vitro gut sacs prepared from the hindgut of the Pacific hagfish Eptatretus stoutii.Concentration-dependent kinetic analysis resulted in a sigmoidal distribution with a maximal (± SE) uptake rate (J max -like) of 70 ± 3 nmol cm −2 h −1 and an affinity constant (K m -like) of 1072 ± 81 μM. Addition of high alanine concentrations to transport assays did not change dipeptide transport rates, indicating that hydrolysis of the dipeptide in mucosal solutions and subsequent uptake via apical amino acid transporters was not occurring, which was further supported by a K m distinct from that of amino acid transport. Transport occurred independent of mucosal pH, but uptake was reduced by 42% in low mucosal sodium. This may implicate cooperation between peptide transporters and sodium-proton exchangers, previously demonstrated in several mammalian and teleost species. Finally, apical L -alanyl-L -alanine uptake rates (i.e., mucosal disappearance) were significantly increased following a meal, demonstrating regulation of uptake. Overall, this examination of dipeptide acquisition in the earliest extant Agnathan suggests evolutionarily conserved mechanisms of transport between hagfish and later-diverging vertebrates such as teleosts and mammals.

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Peptide transporter 1 (PepT1) in fish: A review

Cited by 33 publications

References 81 publications

Characterizing alternative feeds for rainbow trout (O. mykiss) by 1H NMR metabolomics

Characterizing alternative feeds for rainbow trout (O. mykiss) by 1H NMR metabolomics

Integrative Metabolomics for Assessing the Effect of Insect (Hermetia illucens) Protein Extract on Rainbow Trout Metabolism

Acquisition of alanyl‐alanine in an Agnathan: Characteristics of dipeptide transport across the hindgut of the Pacific hagfish Eptatretus stoutii

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