Peptide nucleic acid-ionic self-complementary peptide conjugates: highly efficient DNA condensers with specific condensing mechanism

Cao, Meiwen; Zhao, Wenjing; Zhou, Peng; Xie, Zilong; Sun, Yawei; Xu, Hai

doi:10.1039/c6ra26329a

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Peptides have been of interest as siRNA delivery systems as they are structurally well defined, biodegradable, and often nontoxic and can be prepared through cost-effective synthetic methods. − With peptides, there is also the possibility to incorporate bioinspired sequences that specifically recognize nucleic acids or proteins for targeting purposes. − A common example is the use of cell-penetrating peptides as carriers of siRNA …”

Section: Introductionmentioning

confidence: 99%

Core–Shell Structures from the Coassembly of Lipoprotein-like Nanoparticles and Plasmid DNA for Gene Delivery

et al. 2022

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We reported self-assembled core–shell nanoparticles (NPs) based on lipoprotein-like NPs and plasmid DNA (pDNA). Lipoprotein-like NPs were prepared using cholic acid (CA)-modified lipopeptides. We designed six different lipopeptides with different peptide segments to construct a series of NPs. It was proven that these NPs have different positive surface charges. These NPs could bind pDNA through electrostatic interaction to form core–shell complexes. The interactions between NPs and pDNA were systematically investigated. The number of NP charges determines the strength of the interaction between NPs and pDNA. Thus, various types of core–shell structures, such as loose and dense core–shell NPs, were found in this system. Cytotoxicity test confirmed that the carriers had no toxicity. We also proved that the core–shell structures have a good cell transfection effect. This study would expand the application of lipopeptide assemblies in the gene delivery field, which may lead to the development of peptide-based gene vectors for therapeutic application.

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Section: Introductionmentioning

confidence: 99%

Core–Shell Structures from the Coassembly of Lipoprotein-like Nanoparticles and Plasmid DNA for Gene Delivery

et al. 2022

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“…DNA condensation is usually mediated by certain condensing agents under cooperative noncovalent interactions, and the choice of condensing agents can significantly impact the condensing mechanism and the resultant condensed nanostructure. , For example, cationic surfactants/lipids can induce DNA compaction into globules or closely packed beadlike structures by first binding electrostatically onto the DNA chain and then causing DNA collapse into the compact state via hydrophobic interactions. − Positively charged polyelectrolytes can induce DNA compaction into spherelike aggregates through electrostatic interaction to generate polyion pairs and liberate small counterions for entropy gain. ,, The surfactant- or polyelectrolyte-induced DNA condensation usually results in random folding of the DNA chain. On the contrary, nature gives the most efficient way for DNA condensation in a well-controlled manner to form highly ordered conformations.…”

Section: Introductionmentioning

confidence: 99%

Peptide-Induced DNA Condensation into Virus-Mimicking Nanostructures

Cao

Wang

Zhao

et al. 2018

ACS Appl. Mater. Interfaces

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A series of surfactant-like peptides have been designed for inducing DNA condensation, which are all comprised of the same set of amino acids in different sequences. Results from experiments and molecular dynamics simulations show that the peptide's self-assembly and DNA-interaction behaviors can be well manipulated through sequence variation. With optimized pairing modes between the β-sheets, the peptide of IVAGK can induce efficient DNA condensation into virus-mimicking structures. The condensation involves two steps; the peptide molecules first bind onto the DNA chain through electrostatic interactions and then self-associate into β-sheets under hydrophobic interactions and hydrogen bonding. In such condensates, the peptide β-sheets act as scaffolds to assist the ordered arrangement of DNA, mimicking the very nature of the virus capsid in helping DNA packaging. Such a hierarchy affords an extremely stable structure to attain the highly condensed state and protect DNA against enzymatic degradation. Moreover, the condensate size can be well tuned by the DNA length. The condensates with smaller sizes and narrow size distribution can deliver DNA efficiently into cells. The study helps not only for probing into the DNA packaging mechanism in virus but also delineating the role of peptide self-assembly in DNA condensation, which may lead to development of peptide-based gene vectors for therapeutic applications.

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An amphiphilic peptide with cell penetrating sequence for highly efficient gene transfection

Wang

Nie

Ding

et al. 2020

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Peptide nucleic acid-ionic self-complementary peptide conjugates: highly efficient DNA condensers with specific condensing mechanism

Abstract: Peptide nucleic acid-ionic self-complementary peptide conjugates can induce efficient DNA condensation via base-pairing interaction and peptide association.

Cited by 5 publications

References 39 publications

Core–Shell Structures from the Coassembly of Lipoprotein-like Nanoparticles and Plasmid DNA for Gene Delivery

Core–Shell Structures from the Coassembly of Lipoprotein-like Nanoparticles and Plasmid DNA for Gene Delivery

Peptide-Induced DNA Condensation into Virus-Mimicking Nanostructures

An amphiphilic peptide with cell penetrating sequence for highly efficient gene transfection

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