Peptide Nucleic Acid Films and Capsules: Assembly and Enzymatic Degradation

Becker, Alisa L.; Johnston, Angus P. R.; Caruso, Frank

doi:10.1002/mabi.200900347

Cited by 40 publications

(31 citation statements)

References 24 publications

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“…Diese Methode wurde dadurch weiterentwickelt, dass hybridisierte Peptid-Nukleinsäuren schichtweise als DNA-Hüllen von Mikrokapseln aufgebaut und die Hüllen durch Anwendung einer Nuklease oder Protease als Katalysator für den Abbau der Hüllen aufgespalten wurden. [94] Eine andere Methode zur Entriegelung DNA-funktionalisierter Mikrokapseln umfasste den Einschluss eines sequenzspezifischen Aptamers in den Mikrokapseln und die Öffnung der Kapseln durch die Bildung von Aptamer-Ligand-Komplexen (Abbildung 20). [95] CaCO 3 -Mikropartikel wurden mit dem anti-Sulforhodamin-B-Aptamer beschichtet und dienten so als Kerne für die schichtweise Abscheidung der beiden Polyelektrolyte Polydiallyldimethylammoniumchlorid (PDDA) und Polystyrolsulfonat (PSS).…”

Section: Methodsunclassified

Stimuliresponsive DNA‐funktionalisierte Nano‐ und Mikrocontainer zur schaltbaren und kontrollierten Freisetzung

Lü

Willner

2015

Angewandte Chemie

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Nanotechnology enables the design of materials with outstanding performance. A key element of nanotechnology is the ability to manipulate and control matter on the nanoscale to achieve a certain desired set of specific properties. Here, we discuss recent insight into the formation mechanisms of inorganic nanoparticles during precipitation reactions. We focus on calcium carbonate, and describe the various transient stages potentially occurring on the way from the dissolved constituent ions to finally stable macrocrystals—including solute ion clusters, dense liquid phases, amorphous intermediates, and nanoparticles. The role of polymers in nucleating, templating, stabilizing, and/or preventing these structures is outlined. As a specific example for applied nanotechnology, the properties of cement are shown to be determined by the formation and interlocking of calcium‐silicate‐hydrate nanoplatelets. The aggregation of these platelets into mesoscale architectures can be controlled with polymers.

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Section: Methodsunclassified

Stimuliresponsive DNA‐funktionalisierte Nano‐ und Mikrocontainer zur schaltbaren und kontrollierten Freisetzung

Lü

Willner

2015

Angewandte Chemie

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“…Poly(thymine) was deposited as base monolayer on the microparticles, using electrostatic interactions. [141] Copyright 2010, Wiley-VCH. Subsequently, by applying a layer-by-layer deposition process, three additional bilayers of the duplex (1)/(2) were deposited on the base layer of (1)/(2) using complementary base-pair interactions, and subsequently, the layers were rigidified by crosslinking with the strand (3).…”

Section: Enzyme-responsive Dna Microcapsulesmentioning

confidence: 99%

Synthesis and Applications of Stimuli‐Responsive DNA‐Based Nano‐ and Micro‐Sized Capsules

Liao

Willner

2017

Adv Funct Materials

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Stimuli-responsive, drug-loaded, DNA-based nano-and micro-capsules attract scientific interest as signal-triggered carriers for controlled drug release. The methods to construct the nano-/micro-capsules involve i) the layer-by-layer deposition of signal-reconfigurable DNA shells on drugloaded microparticles acting as templates, followed by dissolution of the core templates; ii) the assembly of three-dimensional capsules composed of reconfigurable DNA origami units; and iii) the synthesis of stimuli-responsive drug-loaded capsules stabilized by DNA−polymer hydrogels. Triggers to unlock the nano-/micro-capsules include enzymes, pH, light, aptamer−ligand complexes, and redox agents. The capsules are loaded with fluorescent polymers, metal nanoparticles, proteins or semiconductor quantum dots as drug models, with anti-cancer drugs, e.g., doxorubicin, or with antibodies inhibiting cellular networks or enzymes over-expressed in cancer cells. The mechanisms for unlocking the nano-/micro-capsules and releasing the drugs are discussed, and the applications of the stimuli-responsive nano-/microcapsules as sense-and-treat systems are addressed. The scientific challenges and future perspectives of nano-capsules and micro-capsules in nanomedicine are highlighted. Drug Delivery groups or duplex DNA or G-quadruplex by photoactive groups may lead to the triggered separation of duplex DNA or G-quadruplex structures ( Figure 1D). For example, disulfide bridges were reported to stabilize DNA duplex structures, while the reduction of the disulfide by thiols led to the separation of the duplex structures. [24][25][26] Also, trans-azobenzene photoisomerizable units linked covalently to duplex nucleic acids or associated with G-quadruplexes via supramolecular interactions, were reported to stabilize these structures, while the cis-azobencene units lack binding affinities (or stabilization effects) to these structures. [27][28][29][30] Photoisomerization of the trans-azobenzene units to the cis-azobenzene configuration, which lacks affinity toward the duplex DNA, results in the release of the photoactive units, in the weakening of the duplex structures, and eventually in their separation. By the cyclic photoisomerization of the photoactive azobenzene units, the double-stranded nucleic acid is then switched between stable and less stable configurations. Alternatively, photodegradable o-nitrobenzyl phosphate ester groups can be incorporated into the duplex DNA structure, and the light-induced separation of the phosphate units may lead to the separation of the duplex DNA. [31] Finally, sequence-specific nucleic acids, known as aptamers, reveal selective recognition properties toward low-molecular-weight substrates (e.g., ATP, cocaine) [32,33] or macromolecular ligands (e.g., thrombin, VEGF). [34,35] The formation of high-affinity complexes between the aptamer and the ligand may then provide a mechanism to separate duplex nucleic acid hybrids [36] (Figure 1E).The structural features of nucleic acids and the possibilities to reconfigure DNA ...

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“…The latter category usually covalently links amino acids to other molecules, either an alkyl chain to form a peptide amphiphile, or to an aromatic group to create π-π interactions between the aromatic groups 25. Design of other systems based on lipids, which primarily act as barriers, or polynucleic acid for information storage and retrieval mechanisms has also been explored 26-27.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Self-Assembly of Nanostructures for Theranostics

Chen¹,

Liang

2012

Theranostics

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Self-assembly of small molecules or macromolecules through non-covalent or covalent bonds to build up supramolecular nanostructures is a prevalent and important process in nature. While most chemists use small molecules to assemble nanostructures with physical or chemical perturbations, nature adopts enzymes to catalyze the reaction to assemble biological, functional nanostructures with high efficiency and specificity. Although enzymatic self-assembly of nanostructures has been remained challenging for chemists, there are still a few examples of using important enzymes to initiate the self-assembly of nanostructures for diagnosis or therapy of certain diseases because down-regulation or overexpression of certain enzymes always associates with abnormalities of tissues/organs or diseases in living body. Herein, we introduce the concept of enzymatic self-assembly and illustrate the design and application of enzyme-catalyzed or -regulated formation of nanostructures for theranostics.

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Peptide Nucleic Acid Films and Capsules: Assembly and Enzymatic Degradation

Cited by 40 publications

References 24 publications

Stimuliresponsive DNA‐funktionalisierte Nano‐ und Mikrocontainer zur schaltbaren und kontrollierten Freisetzung

Stimuliresponsive DNA‐funktionalisierte Nano‐ und Mikrocontainer zur schaltbaren und kontrollierten Freisetzung

Synthesis and Applications of Stimuli‐Responsive DNA‐Based Nano‐ and Micro‐Sized Capsules

Enzymatic Self-Assembly of Nanostructures for Theranostics

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