Peptide NCTX15 derived from spider toxin gland effectively relieves hyperuricemia in mice

Liu, Yixiang; Liu, Naixin; Bian, Wenxin; Zhang, Yue; Wu, Yutong; Peng, Ying; Ru, Zeqiong; Fu, Zhe; Wang, Yinglei; Li, Chao; Yang, Xinwang; Wang, Ying

doi:10.1016/j.bbrc.2023.149222

Cited by 3 publications

(5 citation statements)

References 45 publications

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“…In vitro, NCTX15 demonstrates XO inhibitory activity. In HUA mice, XO activity is also inhibited, with the 1 mg/kg dose of NCTX15 significantly reducing serum XO levels by 52.6 ± 7.08% [70].…”

Section: Spider Venommentioning

confidence: 99%

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Understanding Hyperuricemia: Pathogenesis, Potential Therapeutic Role of Bioactive Peptides, and Assessing Bioactive Peptide Advantages and Challenges

Chen,

Yang,

Rao

et al. 2023

Foods

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Hyperuricemia is a medical condition characterized by an elevated level of serum uric acid, closely associated with other metabolic disorders, and its global incidence rate is increasing. Increased synthesis or decreased excretion of uric acid can lead to hyperuricemia. Protein peptides from various food sources have demonstrated potential in treating hyperuricemia, including marine organisms, ovalbumin, milk, nuts, rice, legumes, mushrooms, and protein-rich processing by-products. Through in vitro experiments and the establishment of cell or animal models, it has been proven that these peptides exhibit anti-hyperuricemia biological activities by inhibiting xanthine oxidase activity, downregulating key enzymes in purine metabolism, regulating the expression level of uric acid transporters, and restoring the composition of the intestinal flora. Protein peptides derived from food offer advantages such as a wide range of sources, significant therapeutic benefits, and minimal adverse effects. However, they also face challenges in terms of commercialization. The findings of this review contribute to a better understanding of hyperuricemia and peptides with hyperuricemia-alleviating activity. Furthermore, they provide a theoretical reference for developing new functional foods suitable for individuals with hyperuricemia.

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Section: Spider Venommentioning

confidence: 99%

“…This promotes the excretion of UA from the kidneys, thereby reducing the SUA level in HUA mice [58]. In HUA mice, NCTX15 downregulates URAT1 and GLUT9 protein expression levels, and upregulates the OAT1 expression level to promote UA excretion [70].…”

Section: Peptides That Regulate the Expression Levels Of Ua Transportersmentioning

confidence: 99%

Understanding Hyperuricemia: Pathogenesis, Potential Therapeutic Role of Bioactive Peptides, and Assessing Bioactive Peptide Advantages and Challenges

Chen,

Yang,

Rao

et al. 2023

Foods

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“…Spider venom comprises various biologically active substances, including small ions, peptides, and proteins with antibacterial and anti-inflammatory properties. Liu et al 117 extracted and characterized a short peptide, NCTX15, with the sequence QSGHTFK, from the Nephila clavata toxin gland. In vitro experiments demonstrated that NCTX15 (1 mg mL −1 ) effectively inhibited XOD activity by 40%.…”

Section: Evaluation Of Bioactive Peptides In Anti-hyperuricemiamentioning

confidence: 99%

“…Furthermore, QSGHTFK demonstrates an alleviation of the inflammatory response and renal injury by inhibiting the expression of inflammatory factors such as IL-6, IL-1β, NLR family, and by attenuating NLRP3 inflammasome-mediated pyroptosis in hyperuricemic mice. 117…”

Section: Evaluation Of Bioactive Peptides In Anti-hyperuricemiamentioning

confidence: 99%

Anti-hyperuricemia bioactive peptides: a review on obtaining, activity, and mechanism of action

Chen,

Li,

Cai

et al. 2024

Food Funct.

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“…They perform the basic functions of venom and help spiders adapt to their ecology. It is crucial to study specific spider toxins to treat spider bites and develop practical applications [ 42 ]. In the following sections, we analyze the multifunctional CAP superfamily.…”

Section: Introductionmentioning

confidence: 99%

Identification and Evolutionary Analysis of the Widely Distributed CAP Superfamily in Spider Venom

Jiang,

Wang,

Zhang

et al. 2024

Toxins

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Venom plays a crucial role in the defense and predation of venomous animals. Spiders (Araneae) are among the most successful predators and have a fascinating venom composition. Their venom mainly contains disulfide-rich peptides and large proteins. Here, we analyzed spider venom protein families, utilizing transcriptomic and genomic data, and highlighted their similarities and differences. We show that spiders have specific combinations of toxins for better predation and defense, typically comprising a core toxin expressed alongside several auxiliary toxins. Among them, the CAP superfamily is widely distributed and highly expressed in web-building Araneoidea spiders. Our analysis of evolutionary relationships revealed four subfamilies (subA-subD) of the CAP superfamily that differ in structure and potential functions. CAP proteins are composed of a conserved CAP domain and diverse C-terminal domains. CAP subC shares similar domains with the snake ion channel regulator svCRISP proteins, while CAP subD possesses a sequence similar to that of insect venom allergen 5 (Ag5). Furthermore, we show that gene duplication and selective expression lead to increased expression of CAP subD, making it a core member of the CAP superfamily. This study sheds light on the functional diversity of CAP subfamilies and their evolutionary history, which has important implications for fully understanding the composition of spider venom proteins and the core toxin components of web-building spiders.

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Peptide NCTX15 derived from spider toxin gland effectively relieves hyperuricemia in mice

Cited by 3 publications

References 45 publications

Understanding Hyperuricemia: Pathogenesis, Potential Therapeutic Role of Bioactive Peptides, and Assessing Bioactive Peptide Advantages and Challenges

Understanding Hyperuricemia: Pathogenesis, Potential Therapeutic Role of Bioactive Peptides, and Assessing Bioactive Peptide Advantages and Challenges

Anti-hyperuricemia bioactive peptides: a review on obtaining, activity, and mechanism of action

Identification and Evolutionary Analysis of the Widely Distributed CAP Superfamily in Spider Venom

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