Peptide mimicking antigenic and immunogenic epitope of neuwiedase from Bothrops neuwiedi snake venom

Cardoso, Rafael M.; Homsi-Brandeburgo, Maria Inês; Rodrigues, Veridiana M.; Santos, Welson Barbosa; Souza, G.L.R.; Prudêncio, Carlos Roberto; Siquieroli, Ana Carolina Silva; Goulart, Luiz Ricardo

doi:10.1016/j.toxicon.2008.11.009

Cited by 25 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Depending on the antigenicity of the different constituent toxins and their relative abundance in the venom, or the venoms used in the immunogen mixture, the antibodies against each toxin type can be found within the antivenoms [27]. The presence of common antigenic regions leads to cross-neutralisation of the snake venom toxins by antibodies raised against different toxins [28]. This occurs most commonly with toxins of the same type (e.g., phospholipases, snake venom metalloproteinases).…”

Section: Discussionmentioning

confidence: 99%

Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms

Silva

Hodgson

2016

Toxins

View full text Add to dashboard Cite

There is limited information on the cross-neutralisation of neurotoxic venoms with antivenoms. Cross-neutralisation of the in vitro neurotoxicity of four Asian and four Australian snake venoms, four post-synaptic neurotoxins (α-bungarotoxin, α-elapitoxin-Nk2a, α-elapitoxin-Ppr1 and α-scutoxin; 100 nM) and one pre-synaptic neurotoxin (taipoxin; 100 nM) was studied with five antivenoms: Thai cobra antivenom (TCAV), death adder antivenom (DAAV), Thai neuro polyvalent antivenom (TNPAV), Indian Polyvalent antivenom (IPAV) and Australian polyvalent antivenom (APAV). The chick biventer cervicis nerve-muscle preparation was used for this study. Antivenom was added to the organ bath 20 min prior to venom. Pre- and post-synaptic neurotoxicity of Bungarus caeruleus and Bungarus fasciatus venoms was neutralised by all antivenoms except TCAV, which did not neutralise pre-synaptic activity. Post-synaptic neurotoxicity of Ophiophagus hannah was neutralised by all antivenoms, and Naja kaouthia by all antivenoms except IPAV. Pre- and post-synaptic neurotoxicity of Notechis scutatus was neutralised by all antivenoms, except TCAV, which only partially neutralised pre-synaptic activity. Pre- and post-synaptic neurotoxicity of Oxyuranus scutellatus was neutralised by TNPAV and APAV, but TCAV and IPAV only neutralised post-synaptic neurotoxicity. Post-synaptic neurotoxicity of Acanthophis antarcticus was neutralised by all antivenoms except IPAV. Pseudonaja textillis post-synaptic neurotoxicity was only neutralised by APAV. The α-neurotoxins were neutralised by TNPAV and APAV, and taipoxin by all antivenoms except IPAV. Antivenoms raised against venoms with post-synaptic neurotoxic activity (TCAV) cross-neutralised the post-synaptic activity of multiple snake venoms. Antivenoms raised against pre- and post-synaptic neurotoxic venoms (TNPAV, IPAV, APAV) cross-neutralised both activities of Asian and Australian venoms. While acknowledging the limitations of adding antivenom prior to venom in an in vitro preparation, cross-neutralization of neurotoxicity means that antivenoms from one region may be effective in other regions which do not have effective antivenoms. TCAV only neutralized post-synaptic neurotoxicity and is potentially useful in distinguishing pre-synaptic and post-synaptic effects in the chick biventer cervicis preparation.

show abstract

Section: Discussionmentioning

confidence: 99%

Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms

Silva

Hodgson

2016

Toxins

View full text Add to dashboard Cite

show abstract

“…the dots are subdivided according to the type of selection (S). the clone location is identified according to its respective lane number (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) and column letter (A-F). the negative control (wild-type M13 phage, without peptide) is located in lane G. the positive control (total tick proteins) is located in lane h. the "-" represent blank dots.…”

Section: Binding Assays: Antigenicity and Specificitymentioning

confidence: 99%

“…12 these mimotopes may represent linear B cell epitopes, conformational epitopes, and conformational discontinuous epitopes of tick proteins, as shown elsewhere. 10,13 Another important and complementary approach for antigen discovery is in silico analysis, which has been used to determine protein features that are important for antigen selection and may also lead to the identification of potential vaccine candidates. 14 in this investigation, we have used the strategy to immunize chickens to generate a very large response to R. microplus proteins, as well as use the hyperimmune sera for a large-scale antigen fingerprinting based on phage display, which generated a reservoir of novel tick antigens with immunogenic potential.…”

mentioning

confidence: 99%

Antigen Fingerprinting of Polyclonal Antibodies Raised in Immunized Chickens with Tick Total Proteins: A Reservoir for the Discovery of Novel Antigens

et al. 2011

View full text Add to dashboard Cite

Identification of tick-protective antigens remains the limiting step in vaccine development. The authors have generated several B cell epitope candidates by fingerprinting Rhipicephalus (Boophilus) microplus proteins that were characterized through bioselection of random peptide phage display libraries against polyclonal antibodies antitick proteins. From 280 clones selected and sequenced, 107 distinct reactive clones were validated by dot-blot assays. Eight consensus motifs were generated, and the most frequent ones were PxxKxH, NxxKxxL, and HTS (68.2%, 65%, and 42%, respectively). The consensus sequences identified potential vaccine targets by alignment with the protein database of R. microplus, which may have putative roles in the host response. Sequences that did not align with known proteins but shared extensive homology among each other were classified as conformational epitopes. Sequence alignments also recognized multiple targets, and the most predominant proteins were identified. Finally, immunized mice sera recognized tick proteins, demonstrating that functional epitope profiles can be identified through selection of phage-displayed peptide libraries with hyperimmune sera and revealing that the epitope-displaying phages can be used as potential vaccine immunogens.

show abstract

“…The inflammatory disorders affecting the intestinal tissues, as ulcerative colitis and Crohn's 2 disease, remain as severe gastrointestinal diseases to be solved [22,23]. Currently, therapeutic 3…”

Section: Discussionmentioning

confidence: 99%

“…Additional evidence was observed on the fact that the amount of TNF was not reduced in the presence of NA 2 EDTA, a chelating agent used to remove bivalent ions, which completely eliminates the biological activity of SVMPs [21]. Furthermore, the polyclonal antibody anti-BmooMP-alpha-I probably binds to different domains from the catalytic domain of the enzyme responsible fot catalyzing the proteolysis of TNF [22,23]. In vivo model for assessment of TNF production.…”

Section: Figurementioning

confidence: 99%

Interação entre uma metaloprotease purificada da peçonha de Bothrops moojeni (BmooMP-alfa-I) e o fator de necrose tumoral (TNF): modelos de estudo in vitro e in vivo.

Silva¹

View full text Add to dashboard Cite

A DEUS pela vida que me foi concedida, pelas oportunidades e conquistas alcançadas. Por me dar forças nos vários momentos difíceis e olhar pelo meu crescimento.Aos meus pais, ERCI e VANILDA, vocês são a minha fonte de inspiração que ensinaram tudo de bom que aprendi em minha vida.A você MÃE que me recebeu desde pequena com seu abraço caloroso e com sábias palavras me ensinou lutar pelos meus objetivos e, sempre com seu sorriso fácil e alegre me deu forças para continuar.A você PAI pela proteção, carinho e principalmente por me ensinar a ter fé. Obrigada por me orientar em meu feito de lutas e incertezas, mas também de muitas esperanças e sonhos.Aos meus irmãos, CARLA e DOUGLAS, com vocês que compartilho em minha vida tantos momentos bons de risadas, brincadeira, festas e, claro meus problemas.Aos meus colegas do LABORATÓRIO DE IMUNOPARASITOLOGIA, pelo trabalho compartilhado, as dúvidas, o aprendizado, as brincadeiras que me fizerem sorrir. Obrigada principalmente pelo apoio nas horas difíceis, somente com ele e a amizade de vocês consegui chegar até aqui. Não citarei nomes para não cometer injustiça com ninguém todos vocês foram muito importantes pra mim e, hoje são meus amigos de coração.Ao meu orientador, Dr. JOSÉ ROBERTO MINEO, por me receber como aluna, pela oportunidade de trabalho, pelo incentivo, por compreender minhas dificuldades, pelo apoio nos momentos de dúvidas. Foi ótimo caminhar ao lado da nossa equipe.Aos funcionários do Programa de Pós-Graduação em Imunologia e Parasitologia, pela colaboração na resolução dos problemas e amizade.

show abstract

Peptide mimicking antigenic and immunogenic epitope of neuwiedase from Bothrops neuwiedi snake venom

Cited by 25 publications

References 26 publications

Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms

Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms

Antigen Fingerprinting of Polyclonal Antibodies Raised in Immunized Chickens with Tick Total Proteins: A Reservoir for the Discovery of Novel Antigens

Interação entre uma metaloprotease purificada da peçonha de Bothrops moojeni (BmooMP-alfa-I) e o fator de necrose tumoral (TNF): modelos de estudo in vitro e in vivo.

Contact Info

Product

Resources

About