“…Serine phosphorylation of the H3K9 histone peptide has a pronounced effect on the K M value for the sirtuin 2 enzymatic activity, resulting in a >200-fold decrease in specificity constant. 36 Interestingly, we have not found any studies describing the effect of phosphorylation on HDAC I, II, and IV class activity, and prompted by the slight preferences of glutamic acid, phosphoserine mimetics, at +2 position over serine, we hypothesized that the phosphorylation can influence the deacetylation by class IIa HDACs. We have synthesized single peptides bearing phosphoserine, glutamate, and serine in +2 position and tested their cleavage by class IIa HDACs.…”