Peptide-mediated microalgae harvesting method for efficient biofuel production

Maeda, Yoshiaki; Tateishi, Takuma; Niwa, Yuta; Muto, Masaki; Yoshino, Tomoko; Kisailus, David; Tanaka, Tsuyoshi

doi:10.1186/s13068-015-0406-9

Cited by 20 publications

(5 citation statements)

References 44 publications

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“…This marine diatom accumulates a large amount of storage lipids in the form of triacylglycerol (TAG) [ 22 ]. Multiomics studies of this diatom have been conducted [ 22 , 23 , 24 ], and these investigations provided useful targets for genetic manipulation to further improve the efficiency of biofuel production [ 25 , 26 ]. The outdoor cultivation study conducted at Kitakyushu (33°92’73”N, 130°74’15”E) in Japan revealed that this diatom showed steady growth in raceway- and column-type bioreactors without temperature control from spring to autumn seasons (April–November).…”

Section: Introductionmentioning

confidence: 99%

Outdoor Cultivation of Marine Diatoms for Year-Round Production of Biofuels

et al. 2017

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Biofuel production using microalgae is believed to have the advantage of continuous year-round production over crop plants, which have strong seasonality. However, actual year-round production of microalgal lipids using outdoor mass cultivation has rarely been demonstrated. In our previous study, it was demonstrated that the oleaginous diatom, Fistulifera solaris, was culturable in outdoor bioreactors from spring to autumn, whereas biomass and lipid production in winter failed because F. solaris did not grow below 15 °C. Therefore, another candidate strain that is culturable in winter is required. In this study, a cold-tolerant diatom, Mayamaea sp. JPCC CTDA0820, was selected as a promising candidate for biofuel production in winter. Laboratory-scale characterization revealed that this diatom was culturable at temperatures as low as 10 °C. Subsequently, F. solaris (April–October) and Mayamaea sp. JPCC CTDA0820 (November–March) were cultured in outdoor open-pond bioreactors, wherein year-round production of diatom lipids was successfully demonstrated. The maximal values of areal productivities of biomass and lipids reached to 9.79 and 1.80 g/(m2 day) for F. solaris, and 8.62 and 0.92 g/(m2 day) for Mayamaea sp. JPCC CTDA0820, respectively. With the combined use of these two diatom species, stable year-round production of microalgal lipids became possible.

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Section: Introductionmentioning

confidence: 99%

Outdoor Cultivation of Marine Diatoms for Year-Round Production of Biofuels

et al. 2017

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“…In this study, we employed frustulin, which is known to localize at the surface of the silica cell walls, as an anchor molecule for peptide display and potentially providing an active interface for TiO 2 nucleation. There are several other types of silica cell wall-associated proteins including silaffin, which are embedded in the silica, and cingulin, which are specifically localized at the girdle bands (lateral face) of the diatom cells .…”

Section: Results and Discussionmentioning

confidence: 99%

“…Genetically Engineered Diatom Cell Walls for Enhanced Titania Deposition. In our previous study, 49 a cell surface display system for F. solaris was established by engineering a silica cell wall-associated protein, frustulin1. For a further enhancement of the deposition of titanium species on the silica cell walls, a titanium-associated peptide (RKKRKKRKKRKKGGGW, referred to as dTi1 peptide), designed by Dickerson et al, 42 was displayed on the surface of the silica cell walls with frustulin1 as the anchor, a green fluorescent protein marker, and a (GGGGS) 3 linker (Figure 3A, see also the experimental section in the Supporting Information).…”

Section: Acs Applied Bio Materialsmentioning

confidence: 99%

Development of Titania-Integrated Silica Cell Walls of the Titanium-Resistant Diatom, Fistulifera solaris

Maeda

Niwa

Tang

et al. 2018

ACS Appl. Bio Mater.

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We report the biological synthesis of titania that is integrated into the silica-based cell walls of a titaniumresistant diatom, Fistulifera solaris. Titania is deposited across the diatom cell walls by simply incubating F. solaris in a culture medium containing a high concentration (2 mM) of a water-soluble organo-titanium compound, titanium(IV) bis(ammonium lactato) dihydroxide (TiBALDH) that would otherwise inhibit the growth of other diatom species. Furthermore, we genetically engineered the interfaces of the diatom cell walls with a titanium-associated peptide, which subsequently increased the Ti/Si atomic ratio by more than 50% (i.e., from 6.2 ± 0.2% to 9.7 ± 0.5%, as identified by inductively coupled plasma−atomic emission spectrometry). The titanium content on the F. solaris silica cell walls is one of the highest reported to date, and comparable to that of chemically synthesized TiO 2 −silica composites. Subsequent thermal annealing at 500 °C in air converted the cell wall-bound titania to nanocrystalline anatase TiO 2 , a highly photocatalytically active phase. We propose that incubation of the titanium-resistant F. solaris with TiBALDH as demonstrated in this study could be a promising bioprocess toward the scalable synthesis of TiO 2 . In addition, the genetic engineering we used to modulate the surface properties of diatom silica cell walls could be extended to synthesize controlled nanomaterials for multiple applications including bioremediation, water purification, and energy conversion/storage.

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“…S8C), demonstrating a lack of specific interactions between the cells of the Z Taq and antiZ Taq strains. Previous studies have shown that the time of contact between interacting partners is an important parameter to consider (50). To test this, the PilA1-Z Taq ΔpilT1 and PilA1-antiZ Taq ΔpilT1 strains were cultivated together and then analyzed for improved sedimentation, in comparison to the strains grown individually.…”

Section: Display Of Polymerizing Affibodies For Affinity-drivenmentioning

confidence: 99%

Surface Display of Small Affinity Proteins on Synechocystis sp. Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1

2018

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Functional surface display of small affinity proteins, namely Affibodies (6.5 kDa), was evaluated for the model cyanobacterium sp. PCC 6803, through anchoring to native surface structures. These structures included confirmed or putative subunits of the type IV pili, the S-layer protein, and the heterologous autotransporter antigen 43 system. The most stable display system was determined to be through C-terminal fusion to PilA1, the major type IV pili subunit in , in a strain unable to retract these pili (Δ). Type IV pili synthesis was upheld, albeit reduced, when fusion proteins were incorporated. However, pili-mediated functions such as motility and transformational competency were negatively affected. Display of Affibodies on and the complementary anti-idiotypic Affibodies on or was able to mediate interspecies cell-cell binding by Affibody complex formation. The same strategy was however not able to drive cell-cell binding and aggregation of-only mixtures. Successful Affibody-tagging of the putative minor pilin PilA4 showed that it locates to the type IV pili in , and that its extracellular availability depends on PilA1. In addition, Affibody-tagging of the S-layer protein indicated that the domains responsible for anchoring and secretion of this protein are located at the N- and C-terminus, respectively. This study can serve as a basis for future surface display of proteins on for biotechnological applications. Cyanobacteria are gaining interest for their potential as autotrophic cell factories. Development of efficient surface display strategies could improve their suitability for large-scale applications by providing options for designed microbial consortia, cell immobilization, and biomass harvesting. Here, surface display of small affinity proteins was realized by fusing them to the major subunit of the native type IV pili in sp. PCC 6803. Display of complementary affinity proteins allowed specific cell-cell binding between and or Additionally, successful tagging of the putative pilin PilA4, helped determine its localization to the type IV pili. Analogous tagging of the S-layer protein shed light on the regions involved in its secretion and surface anchoring.

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Peptide-mediated microalgae harvesting method for efficient biofuel production

Cited by 20 publications

References 44 publications

Outdoor Cultivation of Marine Diatoms for Year-Round Production of Biofuels

Outdoor Cultivation of Marine Diatoms for Year-Round Production of Biofuels

Development of Titania-Integrated Silica Cell Walls of the Titanium-Resistant Diatom, Fistulifera solaris

Surface Display of Small Affinity Proteins on Synechocystis sp. Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1

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