Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica

Abstract

Tethered bilayer lipid membranes (tBLMs) on solid supports have substantial advantages as models of artificial cell membranes for such biomedical applications as drug delivery and biosensing. Compared with untethered lipid membranes, tBLMs have more space between substrate and the bilayer and greater stability. The purpose of this work was to use these properties to fabricate and characterize a zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipid tBLM containing 2 mol% 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide(poly(ethylene glycol))-2000 (DSPE-PEG2000-NHS) lipid tethers on a 3-aminopropyltrimethoxysilane-modified mesoporous silica substrate. A quartz crystal microbalance with dissipation monitoring was used to monitor the process of vesicle adsorption and tBLM self-assembly, and atomic force microscopy was performed to characterize the structural properties of the tBLM obtained. Whereas tether-containing lipid vesicles ruptured neither spontaneously nor as a result of osmotic shock, introduction of an amphipathic α-helical (AH) peptide induced vesicle rupture and subsequent tBLM formation. Taken together, our findings suggest that the AH peptide is an efficient means of rupturing vesicles of both simple and complex composition, and is, therefore, useful for formation of tBLMs on solid and mesoporous materials for applications in biotechnology.

From spherical compartments to polymer films: exploiting vesicle fusion to generate solid supported thin polymer membranes

“…We used QCM-D to investigate: (i) the attachment of polymersomes on gold coated sensors; (ii) the impact of osmotic shock induced by a flow of Ca 2+ -free HEPES buffer (the polymersomes were formed in Ca 2+ enriched HEPES buffer) and if it is able to rupture the polymersomes and initiate the planar membrane formation; and finally, (iii) if the PDMS length (units) influences the formation of solid supported polymer membranes. QCM-D is a powerful technique for analyzing surfaces and commonly used for the study of solid supported membranes, 7,24,27,28,52 especially lipid bilayer formation 5,53,54 and the adsorption of compounds on a surface. QCM-D monitors frequency changes (ΔF) of special quartz sensors in response to changes of the adsorbed mass.…”

Stem cell homing using local delivery of plerixafor and stromal derived growth factor‐1alpha for improved bone regeneration around Ti‐implants

“…, where C is the mass sensitivity constant (17.7 ng⋅Hz −1 ⋅cm −2 ) and n the overtone number (1, 3, 5, and so forth). Prior to analysis, the mesoporous titania matrix was deposited onto Ti QCM‐D sensors (QSX 310, Q‐Sense, Sweden), a methodology proven to result in stable mesoporous thin films . The initial flow in the QCM‐D experiments was pure Milli‐Q H 2 O using a flow rate of 50.0 mL/min until a stable baseline was obtained.…”

“…Prior to analysis, the mesoporous titania matrix was deposited onto Ti QCM-D sensors (QSX 310, Q-Sense, Sweden), a methodology proven to result in stable mesoporous thin films. 30,36,37,[42][43][44][45] The initial flow in the QCM-D experiments was pure Milli-Q H 2 O using a flow rate of 50.0 mL/ min until a stable baseline was obtained. Thereafter, a flow at 50.0 mL/min of either plerixafor (0.4 mg/mL) or SDF-1a (8.0Á10 24 mg/mL) dissolved in water was added to monitor the drug adsorption into the mesoporous film.…”

Tethered Membrane Architectures—Design and Applications

“…Sparsely tethered membranes can be combined with mesoporous substrates to increase control over membrane architecture, composition and hydration (Wallin et al, 2015). By using vesicles comprised of 1-palmitoyl-2-oleoyl-snglycero-3-phosphocholine (POPC) and a small amount of Distearoylphosphatidylethanolamine (DPSE) furnished with a long-chain poly(ethylene) glycol (PEG-2000) tether and an N-hydroxysuccinimide anchor, a lipid bilayer was formed on mesoporous silica functionalized with amines.…”