Peptide identification in alcalase hydrolysated pollen and comparison of its bioactivity with royal jelly

Maqsoudlou, Atefe; Mahoonak, Alireza Sadeghi; Mora, Leticia; Mohebodini, Hossein; Ghorbani, Mansour

doi:10.1016/j.foodres.2018.09.027

Cited by 40 publications

(45 citation statements)

References 47 publications

(60 reference statements)

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“…Many studies have reported the presence of phosphorylated, glycosylated, methylated and deamided proteins in RJ. These post translational modifications could explain the heterogeneous composition of peptides in the RP-HPLC profile of RJ (Maqsoudlou et al, 2018). It has also been reported that major RJ protein genes have also been reported to be present in a honeybee, as they can make them (Zheng et al, 2012).…”

Section: Proteinsmentioning

confidence: 92%

“…Besides the MRJPs, low amounts of several minor proteins including bioactive peptides are present in RJ (Jamnik, Raspor, & Javornik, 2012 (Fontana et al, 2004). Although there have been few studies about the mechanism of peptide generation in RJ, according to a report by Maqsoudlou et al (2018) none of the natural peptides identified in RJ were attributed to honeybees since they originated from the plant species source where the pollen had been collected by them. Therefore it is proposed that the honeybees do not have the gene involved in the production of these peptides.…”

Section: Proteinsmentioning

confidence: 99%

“…Some of these peptides are naturally found in pollen and the honeybee receives them directly by eating pollen (Schönleben et al., 2007). Possibly some of the pollen proteins are converted into peptides in RJ through honeybee enzymes (Maqsoudlou et al, 2018).…”

Section: Proteinsmentioning

confidence: 99%

“…The antioxidant properties have been proven in various experimental models attributed to proteins and peptides, which are potentially associated with the recovery after fatigue (Moriyama, Bagchi, & Bagchi, 2017; Functional properties of Royal jelly 2011a). Considering that RJ is produced after the digestion of bee pollen by natural enzymes in honeybee, and that all pollen phenolic compounds are also found in RJ, the antioxidant activity of RJ can be concluded to be related to phenolic compounds, proteins and peptides (Maqsoudlou et al, 2018). Some studies have been exploring the biological effects of RJ with the use of whole-animal experimental systems in in vitro conditions.…”

Section: Antioxidative Activitymentioning

confidence: 99%

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Royal Jelly: Chemistry, Storage and Bioactivities

Maghsoudlou

Mahoonak

Mohebodini

et al. 2019

Journal of Apicultural Science

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Royal jelly (RJ) has been known for centuries, but in the last 5-6 decades its systematic production and consumption has increased. RJ is secreted by the hypopharyngeal and mandibular glands of worker honeybees (Apis mellifera). This thick and milky substance contains water, proteins, carbohydrates, lipids, minerals, vitamins and such bio-active compounds as acetylcholine, peptides, the hormones testosterone, progesterone, prolactin, estradiol, (hydroxydecanoic acid) (HAD), adenosine monophosphate (AMP)-N1Oxide, polyphenols, flavonoids and adenosine. Because of its bioactive compounds, RJ can be considered as a functional and nutraceutical food. The main goal of this review is to summarize and update its physicochemical properties, bio-active ingredients, storage stability and shelf life. The functional properties are antioxidative activity, insulin-like action, improvement against diabetes, liver protection, antitumoral action, neurotrophic action, antibiotic effect, anti-inflammatory action and wound healing, hypotensive effect and blood regulatory actions, anti-aging effect and skin protection, effects on the reproductive system and fertility and also fortifying, tonic action and immunomodulating and anti-alergic activity. RJ may cause allergic reactions, asthma and even fatal anaphylaxis in some humans. Therefore, RJ should be orally ingested as nutreaceutical agent or food-ingredient only after an allergy test.

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Section: Proteinsmentioning

confidence: 92%

Section: Proteinsmentioning

confidence: 99%

Section: Proteinsmentioning

confidence: 99%

Section: Antioxidative Activitymentioning

confidence: 99%

See 2 more Smart Citations

Royal Jelly: Chemistry, Storage and Bioactivities

Maghsoudlou

Mahoonak

Mohebodini

et al. 2019

Journal of Apicultural Science

Self Cite

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“…4 The antioxidant activity of RJ inhibits oxidative stress and protects DNA against oxidative stress. 5 Dalfardi et al reported the protective effects of RJ against the toxic effects of silver nanoparticles on the hippocampus of male rats. 6 Furthermore, Teixeira et al indicated that the administration of RJ improved the brain antioxidant system in rats.…”

Section: Introductionmentioning

confidence: 99%

Effects of Royal Jelly on the Prefrontal Cortex in a Rat - Morphine Toxicity Model

Jalili

Roshankhah

Mohammadi

et al. 2019

J Apple Biotechnol Rep

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Introduction: Royal jelly (RJ) is a honey bee secretion with numerous medicinal properties and antioxidant activities. Morphine is a major risk factor in the development of functional disorders in several organ systems. This study was designed to evaluate the effects of RJ against morphineinduced damage to the prefrontal cortex of rats. Materials and Methods: In this study, 48 male Wistar rats were randomly assigned into 6 groups: sham group, morphine group, RJ groups (100, and 200 mg/kg), and morphine + RJ groups. Treatments were administered intraperitoneally and orally for 20 days on a daily basis. Ferric reducing/ antioxidant power (FRAP) method was applied to determine the total antioxidant capacity. The number of neurons and, dendritic spines were investigated by Golgi technique, and Griess technique was employed to determine the serum nitrite oxide level. Results: Morphine administration significantly increased the nitrite oxide level and total antioxidant capacity, and reduced neuronal dendritic spines and neurons compared to the sham group (P < 0.05). In all RJ and Morphine + RJ groups, the number of neurons and neuronal dendritic spines were elevated significantly, while nitrite oxide level and total antioxidant capacity were reduced compared to the morphine group (P < 0.05). Conclusions: RJ administration protected animals against oxidative stress and nitrite oxide. It also improves some prefrontal cortex parameters including number of neurons and dendritic spines because of the morphine.

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Physicochemical, antioxidant, antimicrobial, and in vitro cytotoxic activities of corn pollen protein hydrolysates obtained by different peptidases

Akbarmehr

Peighambardoust

Ghanbarzadeh

et al. 2023

Food Science & Nutrition

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The applications of protein hydrolysates as food preservatives and nutraceutical ingredients have attracted much attention because of their beneficial effects. The interest in these ingredients has shifted toward their biological activities with benefits to human health. Bioactive peptides are known as antioxidant agents that could promote health‐promoting effects and prolong food shelf‐life beyond their basic nutritional value. Thus, the aim of this study was to investigate antioxidant, antimicrobial, and in vitro cytotoxic properties of corn pollen protein (CPP) hydrolysates obtained by different enzymes. Proteolytic activity in terms of degree of hydrolysis (DH) and SDS‐PAGE analysis was measured in pancreatin (H‐Pan), pepsin (H‐Pep), and trypsin (H‐Tri) hydrolysates. Amino acid composition, antioxidant and antimicrobial activities, and cytotoxicity of hydrolysates were evaluated. DH and SDS‐PAGE revealed higher proteolytic activity of pepsin compared to other enzymes. Amino acid analysis showed that the functional amino acids such as antioxidant types were most predominant in H‐Pep compared to two other samples. Antioxidant activity of hydrolysates was found to be affected by the type of enzyme and the concentration of hydrolysates. There was a significant difference ( p < 0.05) between antioxidant activity of different hydrolysates. The highest antioxidant activity in terms of Trolox equivalent antioxidant capacity (0.23–2.75 mM), DPPH (33.3%–64.8%), and hydroxyl (33.7%–63.2%) radical scavenging activities, chelation of iron (33.2%–62.5%) and copper (30.2%–50.5%) metals, and total antioxidant activity (0.65–0.85) was obtained for H‐Pep followed by H‐Pan and H‐Tri samples. Antibacterial tests showed that pepsin‐hydrolyzed protein was not significantly ( P > 0.05) effective against E. coli at any concentrations, however, it showed significant ( P < 0.05) concentration‐dependent effect against S. aureus (with inhibition zones of 15–25 mm). Cytotoxicity results revealed that CPP, as a nonhydrolyzed protein, did not generally show antiproliferative activity, however, a significant ( P < 0.05) ability of H‐Pep hydrolysate in decreasing HT‐29 colon cancer cell line viability was seen in a concentration‐dependent manner (the lowest cell viability of 32% at 5 mg/mL). Overall, investigating the application of protein‐based hydrolysates is one of the possible strategies that govern their applied intentions as preservatives and nutraceuticals in the food and pharmaceutical industries.

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Peptide identification in alcalase hydrolysated pollen and comparison of its bioactivity with royal jelly

Cited by 40 publications

References 47 publications

Royal Jelly: Chemistry, Storage and Bioactivities

Royal Jelly: Chemistry, Storage and Bioactivities

Effects of Royal Jelly on the Prefrontal Cortex in a Rat - Morphine Toxicity Model

Physicochemical, antioxidant, antimicrobial, and in vitro cytotoxic activities of corn pollen protein hydrolysates obtained by different peptidases

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