Peptide‐Functionalized, Low‐Biofouling Click Multilayers for Promoting Cell Adhesion and Growth

Abstract

A universal method of assembling low‐biofouling films that can be engineered to promote the specific adhesion and growth of cells onto surfaces is described (see image). Click chemistry and the layer‐by‐layer method are used to assemble films of poly(ethylene glycol) acrylate, which are functionalized with the adhesion‐promoting peptide, arginine‐glycine‐aspartate (RGD).

Particle Targeting in Complex Biological Media

“…[124b,133] For an even higher PEG proportion, multilayered PEG capsules can be generated via LbL assembly and click chemistry, using alkyne-and azide-functionalized PEG molecules (PEG Alk and PEG Az ) as building blocks (Figure 12). [130,134] When crosslinked with a disulfide cleavable linker, the PEGbased multilayered capsules exhibited specific deconstruction under reducing conditions and low-fouling properties with negligible cytotoxicity. [130] Further post-functionalization of the PEG surface with targeting ligands, e.g., RGD or scFv, was also achieved, providing multilayered capsules with targeting ability and enabling the capsules to be used as targeted drug carriers.…”

“…[130] Further post-functionalization of the PEG surface with targeting ligands, e.g., RGD or scFv, was also achieved, providing multilayered capsules with targeting ability and enabling the capsules to be used as targeted drug carriers. [133,134] When preparing these types of multilayered capsules through LbL assembly, it has been shown that the assembly method used can substantially affect the resulting particles; therefore particles with tailored properties (but composed of the same materials) can be engineered through judicious choice of the assembly technology. [150] Additionally, PEG hydrogel particles with controllable size and tunable elasticity can be engineered using a MS templating method.…”

“…Reproduced with permission. [134] www.advancedsciencenews.com www.advhealthmat.de also be assembled from PDPA and PEG block copolymers which, after functionalization with peptides, can target atherosclerotic plaques. [152] Other neutral or zwitterionic polymers have also been used as building blocks to generate ultralowfouling drug carrier systems.…”

“…[59] This approach has been reported as an efficient method for attaching different targeting ligands, including Man, scFv, Tf, and polypeptides. [59,133,134,158] However, PEG itself could affect the efficiency of conjugating the ligand to the particle. Bargheer et al reported that higher PEGylation of iron oxide NPs resulted in diminished binding of Tf onto PEGylated particle surfaces.…”

In-situ monitoring of drug release from therapeutic eluting polyelectrolyte multilayers under static and dynamic conditions

“…Therapeutic incorporation at significant doses in biocompatible and biodegradable LbL host and their controlled release under physiological conditions are at the frontier of the rapidly advancing LbL field [5,6]. A large body of literature exists that covers a diverse range of therapeutic agents such as small drugs [7], peptides [8,9], proteins [10,11] and nucleic acids [12] at mild aqueous conditions. LbL can be incorporated in the drug delivery structures in multiple ways.…”