Peptide defenses of the Cascades frog Rana cascadae: implications for the evolutionary history of frogs of the Amerana species group

Conlon, J. Michael; Al-Dhaheri, Ahmed S.; al-Mutawa, Eissa; al-Kharrge, Rokaya; Ahmed, Eman Farouk; Kolodziejek, Jolanta; Nowotny, Norbert; Nielsen, Per F.; Davidson, Carlos

doi:10.1016/j.peptides.2007.03.010

Cited by 29 publications

(15 citation statements)

References 32 publications

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“…ToAP2S1 was also tested to evaluate if it also has lost its hemolytic activity. NDBP-4.23 and ToAP1 might be considered of interest to future studies, because in all the tested concentrations their hemolysis curves were significantly lower than 50% of hemolysis (Conlon et al, 2007; Thaker et al, 2011; Figures 3B,C ). However, NDBP-5.7 presents a hemolysis percent higher than 50% in concentrations up to 50 μM ( Figure 3D ).…”

Section: Resultsmentioning

confidence: 99%

Activity of Scorpion Venom-Derived Antifungal Peptides against Planktonic Cells of Candida spp. and Cryptococcus neoformans and Candida albicans Biofilms

et al. 2016

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The incidence of fungal infections has been increasing in the last decades, while the number of available antifungal classes remains the same. The natural and acquired resistance of some fungal species to available therapies, associated with the high toxicity of these drugs on the present scenario and makes an imperative of the search for new, more efficient and less toxic therapeutic choices. Antimicrobial peptides (AMPs) are a potential class of antimicrobial drugs consisting of evolutionarily conserved multifunctional molecules with both microbicidal and immunomodulatory properties being part of the innate immune response of diverse organisms. In this study, we evaluated 11 scorpion-venom derived non-disulfide-bridged peptides against Cryptococcus neoformans and Candida spp., which are important human pathogens. Seven of them, including two novel molecules, showed activity against both genera with minimum inhibitory concentration values ranging from 3.12 to 200 μM and an analogous activity against Candida albicans biofilms. Most of the peptides presented low hemolytic and cytotoxic activity against mammalian cells. Modifications in the primary peptide sequence, as revealed by in silico and circular dichroism analyses of the most promising peptides, underscored the importance of cationicity for their antimicrobial activity as well as the amphipathicity of these molecules and their tendency to form alpha helices. This is the first report of scorpion-derived AMPs against C. neoformans and our results underline the potential of scorpion venom as a source of antimicrobials. Further characterization of their mechanism of action, followed by molecular optimization to decrease their cytotoxicity and increase antimicrobial activity, is needed to fully clarify their real potential as antifungals.

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Section: Resultsmentioning

confidence: 99%

Activity of Scorpion Venom-Derived Antifungal Peptides against Planktonic Cells of Candida spp. and Cryptococcus neoformans and Candida albicans Biofilms

et al. 2016

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“…4b). Temporin-ECa isolated from E. cyanophlyctis is more similar 69.23% to temporin-1CSc from Rana muscosa and 61.54% to temporin-1 M from Rana cascadae (Rollins-Smith et al 2006;Conlon et al 2007) (Fig. 4c).…”

Section: Homology and Phylogenymentioning

confidence: 84%

Identification and Characterization of Novel Antibacterial Peptides from Skin Secretions of Euphlyctis cyanophlyctis

Asoodeh

Ghorani‐Azam

Chamani

2011

Int J Pept Res Ther

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In this study, we extracted and purified antimicrobial peptides (AMPs) secreted from skin of Euphlyctis cyanophlyctis using reverse phase-high performance liquid chromatography. Three AMPs were isolated from skin secretions of this frog and sequenced using tandem mass spectrometry. The purified peptides were named buforin-EC (1875.05 ± 0.5 Da), cyanophlyctin (2347.50 ± 0.5 Da) and temporin-ECa (1013.33 ± 0.5 Da). Multiple alignments and homology search showed that buforin-EC, cyanophlyctin and temporin-ECa had a homology of 71.43, 47.1, and 69.23% to buforin II, brevinin-2EC, and temporin-1CSc, respectively. Antimicrobial tests demonstrated that our peptides have a great antimicrobial effect on both grampositive and gram-negative bacteria. The results indicated that they have an overall minimum inhibitory concentration (MIC) below 13 lM against E. coli. No hemolysis was observed in around of their MIC values. In conclusion, skin secretions of E. cyanophlyctis contain a novel class of AMPs with the proper characteristics.

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“…These peptides protect the animal against invasion by a wide range of pathogenic microorganisms [5][6][7]. The skin of amphibian species is a particularly rich source of AMPs; more than 22 kinds of AMPs have already been identified in the skin secretion from a single species of ranid frog, Rana palustris [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, several reports have been published confirming the identification of AMPs from the skin of ranid frogs. For example, ten AMPs have been found from the skin secretions of Rana dybowskii collected on Tsushima Island, Japan [8,9]. An additional six AMPs have been identified from the skin of R. dybowskii in Korea [10].…”

Section: Introductionmentioning

confidence: 99%

Identification and characterisation of a novel antimicrobial polypeptide from the skin secretion of a Chinese frog (Rana chensinensis)

Jin

Song

et al. 2009

International Journal of Antimicrobial Agents

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Peptide defenses of the Cascades frog Rana cascadae: implications for the evolutionary history of frogs of the Amerana species group

Cited by 29 publications

References 32 publications

Activity of Scorpion Venom-Derived Antifungal Peptides against Planktonic Cells of Candida spp. and Cryptococcus neoformans and Candida albicans Biofilms

Activity of Scorpion Venom-Derived Antifungal Peptides against Planktonic Cells of Candida spp. and Cryptococcus neoformans and Candida albicans Biofilms

Identification and Characterization of Novel Antibacterial Peptides from Skin Secretions of Euphlyctis cyanophlyctis

Identification and characterisation of a novel antimicrobial polypeptide from the skin secretion of a Chinese frog (Rana chensinensis)

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