Peptide Conjugated Magnetic Nanoparticles for Magnetically Mediated Energy Delivery to Lung Cancer Cells

Abstract

Aim:In the present study, we examine the effects of internalized peptide-conjugated iron oxide nanoparticles and their ability to locally convert alternating magnetic field (AMF) energy into other forms of energy (e.g., heat and rotational work). Materials & methods: Dextran-coated iron oxide nanoparticles were functionalized with a cell penetrating peptide and after internalization by A549 and H358 cells were activated by an AMF. Results: TAT-functionalized nanoparticles and AMF exposure increased reactive oxygen species generation compared with the nanoparticle system alone. The TAT-functionalized nanoparticles induced lysosomal membrane permeability and mitochondrial membrane depolarization, but these effects were not further enhanced by AMF treatment. Although not statistically significant, there are trends suggesting an increase in apoptosis via the Caspase 3/7 pathways when cells are exposed to TATfunctionalized nanoparticles combined with AMF. Conclusion: Our results indicate that internalized TAT-functionalized iron oxide nanoparticles activated by an AMF elicit cellular responses without a measurable temperature rise.

Ferrite Nanoparticles-Based Reactive Oxygen Species-Mediated Cancer Therapy

“…The alternating magnetic field is the most commonly used due to its large penetration. Hilt et al showed that peptide-conjugated magnetic nanoparticles (TAT-IONP) could increase cellular ROS generation in both A549 and H358 cell lines upon exposure to an alternating magnetic field, resulting in an increase in apoptosis via the Caspase 3/7 pathways (Hauser et al, 2016a ). Orel et al designed magnetic nanodots composed of doxorubicin-loaded Fe 3 O 4 nanoparticles, which could release more free iron to promote the formation of highly reactive oxygen species combined with electromagnetic fields, achieving remote modulation of redox state of Walker-256 carcinosarcoma tumor for cancer nanotherapy (Orel et al, 2018 ).…”

Superparamagnetic Iron Oxide Nanoparticles Modified with Silica Layers as Potential Agents for Lung Cancer Treatment

“…Conventionally, SPIONs are destined for subcutaneous or intravenous administration [ 19 ]. However, in the case of lung cancer, pulmonary delivery of the drug is more effective, allows for the reduction of systemic toxicity of the treatment and increases patients’ compliance [ 20 ].…”

A facile one-pot synthesis of poly(acrylic acid)-functionalized magnetic iron oxide nanoparticles for suppressing reactive oxygen species generation and adsorption of biocatalyst

“…Dextran-coated IONPs were functionalized with a cell penetrating peptide and used to inhibit the excessive formation of ROS for the treatment of lung cancer [21]. In recent years, several possible functionalizing agents, such as poly(acrylic-co-maleic), poly(trolox ester) polymers, natural antioxidants (quercetin, curcumin,green tea polyphenols, and anthocyanins),ascorbic, citric, humic, gallic, and polyacrylic acids have also been used to functionalize the bare surface of IONPs to improve colloidal stability and surface properties [20][21][22][23]. Among these surface functionalizing agents, poly(acrylic acid) (PAA) provides both electrostatic and steric repulsions against particles to stabilize their dispersion via surface functionalization of bare IONPs [24].…”