Peptide and Protein Analysis Using Ion Mobility–Mass Spectrometry

Enders, Jeffrey R.; Kliman, Michal; Sundarapandian, Sevugarajan; McLean, John A.

doi:10.1002/9781118116555.ch6

Cited by 3 publications

(1 citation statement)

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“…46 It has the additional benefit of being highly adaptable to preseparation protocols like gas chromatography and ion mobility-mass spectrometry. 47–51…”

Section: Detection Methodsmentioning

confidence: 99%

Amino acids as metabolic substrates during cardiac ischemia

Drake

Sidorov

McGuinness

et al. 2012

Exp Biol Med (Maywood)

111

104

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The heart is well known as a metabolic omnivore in that it is capable of consuming fatty acids, glucose, ketone bodies, pyruvate, lactate, amino acids and even its own constituent proteins, in order of decreasing preference. The energy from these substrates supports not only mechanical contraction, but also the various transmembrane pumps and transporters required for ionic homeostasis, electrical activity, metabolism and catabolism. Cardiac ischemia – for example, due to compromise of the coronary vasculature or end-stage heart failure – will alter both electrical and metabolic activity. While the effects of myocardial ischemia on electrical propagation and stability have been studied in depth, the effects of ischemia on metabolic substrate preference has not been fully appreciated: oxygen deprivation during ischemia will significantly alter the relative ability of the heart to utilize each of these substrates. Although changes in cardiac metabolism are understood to be an underlying component in almost all cardiac myopathies, the potential contribution of amino acids in maintaining cardiac electrical conductance and stability during ischemia is underappreciated. Despite clear evidence that amino acids exert cardioprotective effects in ischemia and other cardiac disorders, their role in the metabolism of the ischemic heart has yet to be fully elucidated. This review synthesizes the current literature of the metabolic contribution of amino acids during ischemia by analyzing relevant historical and recent research.

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“…46 It has the additional benefit of being highly adaptable to preseparation protocols like gas chromatography and ion mobility-mass spectrometry. 47–51…”

Section: Detection Methodsmentioning

confidence: 99%

Amino acids as metabolic substrates during cardiac ischemia

Drake

Sidorov

McGuinness

et al. 2012

Exp Biol Med (Maywood)

111

104

View full text Add to dashboard Cite

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Multidimensional Separations by Ion Mobility‐Mass Spectrometry

Hines

Enders

McLean

2012

Encyclopedia of Analytical Chemistry

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Ion mobility‐mass spectrometry (IM‐MS) couples gas‐phase structural separations by IM with mass‐to‐charge separations by MS. The utility of this integration of multidimensional separation dimensions is manifold, including (i) high throughput multidimensional separations, (ii) separation of chemical noise, and (iii) rapid information in structural biology. This article introduces the unique advantages of IM‐MS in biological fields ranging from systems biology to structural biology, the context of which is introduced by a historical perspective of key advances accomplished over the last century. Contemporary and emerging technology for performing IM‐MS is presented, and applications using conformational data sets in proteomics, glycomics, and lipidomics are described.

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