Peptide and glycopeptide dendrimers. Part II

Vepřek, Pavel; Ježek, Jan

doi:10.1002/(sici)1099-1387(199905)5:5<203::aid-psc181>3.0.co;2-n

Cited by 65 publications

(39 citation statements)

References 144 publications

(145 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MAP peptides are known to elicit strong antibody responses with higher titers compared with the monomeric peptide coupled to a carrier protein (11). A rabbit polyclonal antibody was raised against this phospho-MAP peptide by Capralogics.…”

Section: Methodsmentioning

confidence: 99%

Smooth Muscle Archvillin Is an ERK Scaffolding Protein

Gangopadhyay

Kengni

Appel

et al. 2009

Journal of Biological Chemistry

View full text Add to dashboard Cite

ERK influences a number of pathways in all cells, but how ERK activities are segregated between different pathways has not been entirely clear. Using immunoprecipitation and pulldown experiments with domain-specific recombinant fragments, we show that smooth muscle archvillin (SmAV) binds ERK and members of the ERK signaling cascade in a domain-specific, stimulus-dependent, and pathway-specific manner. MEK binds specifically to the first 445 residues of SmAV. B-Raf, an upstream regulator of MEK, constitutively interacts with residues 1-445 and 446 -1250. Both ERK and 14-3-3 bind to both fragments, but in a stimulus-specific manner. Phosphorylated ERK is associated only with residues 1-445. An ERK phosphorylation site was determined by mass spectrometry to reside at Ser 132 . A phospho-antibody raised to this site shows that the site is phosphorylated during ␣-agonist-mediated ERK activation in smooth muscle tissue. Phosphorylation of SmAV by ERK decreases the association of phospho-ERK with SmAV. These results, combined with previous observations, indicate that SmAV serves as a new ERK scaffolding protein and provide a mechanism for regulation of ERK binding, activation, and release from the signaling complex.

show abstract

Section: Methodsmentioning

confidence: 99%

Smooth Muscle Archvillin Is an ERK Scaffolding Protein

Gangopadhyay

Kengni

Appel

et al. 2009

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…72 The dendrimer design was inspired by the use of polylysine dendrimers as scaffolds for the presentation of peptide antigens (multiple antigenic peptide systems (MAPs)) in order to promote the production of antibodies to the proteins from which the peptide was derived. 73,74 Thus, Nomizu prepared a series of polylysine dendrimers functionalized with 16 ((Ac-YIGSR) 16 K 8 K 4 K 2 KG -designated Y16), 8 ((Ac-YIGSR) 8 K 4 K 2 KG -designated Y8), and 4 ((Ac-YIGSR) 4 K 2 KG -designated Y4) peptides. Lung colony counts in mice co-injected with B16-F10 melanoma cells and Y16 dendrimer were reduced by 97% from control.…”

Section: Peptide-functionalised Dendrimers As Inhibitors Of Metastasismentioning

confidence: 99%

Dendrimers for Biomedical Applications

Kaminskas

McLeod

Jones

et al. 2014

Frontiers in Nanobiomedical Research

View full text Add to dashboard Cite

Dendrimers are globular polymers with well-defi ned molecular structures and polyfunctional surfaces. The ability to functionalize the surface of dendrimers with a range of chemical species and to tailor physicochemical properties has resulted in the exploration of dendrimers for a wide range of biomedical applications. The clinical application of dendrimers is still in the early stages of development, although data generated thus far have suggested that they have the potential to advance the fi elds of drug delivery, gene therapy, imaging and wound healing. This chapter provides an overview of the current state-of-the-art of research into the biomedical application of dendrimers.

show abstract

“…However, the amplification factor can easily lead to nanomolar dissociation constants for the assembly as a whole when single affinities are in the millimolar to micromolar range. The MAP strategy affords construction of multivalent assemblies with an exceptional degree of flexibility as illustrated in Figure 15.5 [32,33,35,36]. MAPs containing monoepitopes, chimeric epitopes, and diepitopes can be generated at will.…”

Section: Thiol Chemistrymentioning

confidence: 99%