Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification

Abstract

The interest in therapeutic monoclonal antibodies (mAbs) has significantly grown in the pharmaceutical industry, exceeding 100 FDA mAbs approved. Although the upstream processing of their industrial production has been significantly improved in the last years, the downstream processing still depends on immobilized protein A affinity chromatography. The high cost, low capacity and short half-life of immobilized protein A chromatography matrices, encouraged the design of alternative short-peptide ligands for mAb purification. Most of these peptides have been obtained by screening combinatorial peptide libraries. These low-cost ligands can be easily produced by solid-phase peptide synthesis and can be immobilized on chromatographic supports, thus obtaining matrices with high capacity and selectivity. Furthermore, matrices with immobilized peptide ligands have longer half-life than those with protein A due to the higher stability of the peptides. In this review the design and synthesis of peptide ligands, their immobilization on chromatographic supports and the evaluation of the affinity supports for their application in mAb purification is described.

Rapid Point-of-Care Tests Using Staphylococcal Protein A Can Detect Early IgM Responses in HIV-1 and Treponema pallidum Infections

“…Contributing to the pathogenesis of infection, this effect of SpA is mediated by its natural ability to closely interact with cell-surface and circulating immunoglobulins, primarily of IgG class but also other immunoglobulin classes, in humans and animals ( 27 , 28 ). The distinct feature of having unconventional binding affinity to mammalian immunoglobulins has uniquely positioned SpA for various applications in immunobiological research and biomedical technologies, including immunodiagnostics and affinity chromatography ( 15 , 29 ).…”

Compendium on Food Crop Plants as a Platform for Pharmaceutical Protein Production

“…Generally, recombinant proteins (synthesized in any system) intended for human or animal therapy must be of a high degree of purity. Hence, the purification procedure is focused on obtaining proteins free from any contamination, while maintaining their correct chemical structure and biological function [ 67 , 68 ]. The cost of protein purification accounts for 45% to 92% of the overall manufacturing process [ 1 ].…”

“…When planning plant-derived protein purification strategies, other factors such as high recovery, ease, and repeatability should be considered in addition to economic considerations. In order to meet the strict purity requirements of the biopharmaceutical industry in the production of therapeutics, chromatography is used during purification of the protein product [ 67 ]. Chromatography is undeniably the method of choice, although it is mostly used for small-scale purification of recombinant proteins.…”

Machine Learning Model for Biomimetic Chromatography Peptide Ligands

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“…BC thus addresses the challenges and limitations of traditional AC. The discovery and development of peptide ligands for BC relies on combinatorial approaches and screening libraries for identifying promising leads . A variety of screening methods is available to identify peptide sequences that bind with a particular substrate.…”