Peptic and Tryptic Digestion Products as Inexpensive Culture Mediums for Routine Bacteriologic Work

Bacterial Spores Ii. A Study of Bacterial Spore Germination in Relation to Environment

“…Although their work was not directly concerned with spore germination, it is readily applicable to this process on the basis of the foregoing assumption that spores are not inert, but active bodies having the same or more exacting requirements for their metabolism than do vegetative cells. Stickel and Meyer (1918) suggested the general use of peptic or tryptic digestion products of protein material in bacteriological work, particularly in the cultivation of the sporulating anaerobes, due to the high amino acid content and nitrogen availability of such mediums as compared with the usual standard mediu;ms. Among the numerous investigators suggesting the use of various forms of yeast autolysate or extract mediums, either because of their growth-stimulating properties or as economical substitutes for meat extracts, are Kressler (1918), Kligler (1919), Eberson (1920) and Ayers and Rupp (1920).…”