Pepsinogen C: A Possible Biological Marker of Epithelial Differentiation in Barrett's Esophagus

Vizoso, F; P, Vérez; Andicoechea, Alejandro; Quintela, I; Alexandre, E; Merino, Antonio

doi:10.1177/172460080101600210

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…In addition, pepsinogen C (PGC)is expressed only in the mucosa of the gastric fundus and is expressed by all regions of the gastric mucosa. In a preliminary study, PGC was expressed by columnar non goblet cells in most areas without specialized intestinal metaplasia which can help clinicians identify high risk BE patients and guide endoscopic surveillance 36 . Semaphorin 3C (SEMA3C) has been reported to drive a number of oncogenic programs, correlate poor cancer prognosis, and promote the progression of multiple different cancer types 37 , 38 .…”

Section: Discussionmentioning

confidence: 99%

Analysis of immune related gene expression profiles and immune cell components in patients with Barrett esophagus

Shi

Guo

Chen

et al. 2022

Sci Rep

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Barrett's esophagus (BE) is a well-known precancerous condition of esophageal adenocarcinoma. However, the immune cells and immune related genes involved in BE development and progression are not fully understood. Therefore, our study attempted to investigate the roles of immune cells and immune related genes in BE patients. The raw gene expression data were downloaded from the GEO database. The limma package in R was used to screen differentially expressed genes (DEGs). Then we performed the least absolute shrinkage and selection operator (LASSO) and random forest (RF) analyses to screen key genes. The proportion of infiltrated immune cells was evaluated using the CIBERSORT algorithm between BE and normal esophagus (NE) samples. The spearman index was used to show the correlations of immune genes and immune cells. Receiver operating characteristic (ROC) curves were used to assess the diagnostic value of key genes in BE. A total of 103 differentially expressed immune-related genes were identified between BE samples and normal samples. Then, 7 genes (CD1A, LTF, FABP4, PGC, TCF7L2, INSR,SEMA3C) were obtained after Lasso analysis and RF modeling. CIBERSORT analysis revealed that resting CD4 T memory cells and gamma delta T cells were present at significantly lower levels in BE samples. Moreover, plasma cell and regulatory T cells were present at significantly higher levels in BE samples than in NE samples. INSR had the highest AUC values in ROC analysis. We identified 7 immune related genes and 4 different immune cells in our study, that may play vital roles in the occurrence and development of BE. Our findings improve the understanding of the molecular mechanisms of BE.

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Section: Discussionmentioning

confidence: 99%

Analysis of immune related gene expression profiles and immune cell components in patients with Barrett esophagus

Shi

Guo

Chen

et al. 2022

Sci Rep

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Regulation of Cdx2 expression by promoter methylation, and effects of Cdx2 transfection on morphology and gene expression of human esophageal epithelial cells

et al. 2006

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Caudal-related homeobox 2 (Cdx2) has been suggested as an early marker of Barrett's esophagus (BE), which is the premalignant lesion of esophageal adenocarcinoma (EAC). However, the mechanism of ectopic Cdx2 expression in the esophageal epithelial cells and its role in the development of BE remained unclear. RT-PCR, pyrosequencing and methylation-specific PCR were used to determine expression and promoter methylation of Cdx2 in human esophageal epithelial cells (HET1A and SEG1) after treatment with 5-aza-2'-deoxycytidine (DAC), acid, bile acids and their combination. HET1A cells with stable transfection of Cdx2 were characterized for morphology and gene expression profiles with Affymetrix array. We found Cdx2 was expressed in most human EAC cell lines, but not in squamous epithelial cell lines. DAC-induced demethylation and expression of Cdx2 in HET1A and SEG1 cells, and treatment with a DNA methylating agent counteracted the effect of DAC. Treatment of HET1A and SEG1 cells with acid, bile acids or both also resulted in promoter demethylation and expression of Cdx2. HET1A cells with stable transfection of human Cdx2 formed crypt-like structures in vitro. Microarray analysis and quantitative real-time PCR showed that stable transfection of Cdx2 up-regulated differentiation markers of intestinal columnar epithelial cells and goblet cells in HET1A cells. This may be partially due to modulation of Notch signaling pathway, as western blotting confirmed down-regulation of Hes1 and up-regulation of Atoh1 and Muc2. Our data suggest that exposure to acid and/or bile acids may activate Cdx2 expression in human esophageal epithelial cells through promoter demethylation, and ectopic Cdx2 expression in esophageal squamous epithelial cells may contribute to intestinal metaplasia of the esophagus.

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Pepsinogen C: A Possible Biological Marker of Epithelial Differentiation in Barrett's Esophagus

Cited by 2 publications

References 8 publications

Analysis of immune related gene expression profiles and immune cell components in patients with Barrett esophagus

Analysis of immune related gene expression profiles and immune cell components in patients with Barrett esophagus

Regulation of Cdx2 expression by promoter methylation, and effects of Cdx2 transfection on morphology and gene expression of human esophageal epithelial cells

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