Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

Abstract

The structure of pepsin-solubilized collagen (PSC) obtained from the skin of Lophius litulon was analyzed using the sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). SDS-PAGE results showed that PSC from Lophius litulon skin was collagen type I and had collagen-specific α1, α2, β, and γ chains. FTIR results indicated that the infrared spectrum of PSC ranged from 400 to 4000 cm−1, with five main amide bands. SEM revealed the microstructure of PSC, which consisted of clear fibrous and porous structures. In vitro antioxidant studies demonstrated that PSC revealed the scavenging ability for 2,2-diphenyl-1-picrylhydrazyl (DPPH), HO·, O2−·, and ABTS·. Moreover, animal experiments were conducted to evaluate the biocompatibility of PSC. The collagen sponge group showed a good biocompatibility in the skin wound model and may play a positive role in the progression of the healing process. The cumulative results suggest that collagen from the skin of Lophius litulon has potential applications in wound healing due to its good biocompatibility.

Perspective on the Therapeutic Applications of Algal Polysaccharides

“…Other biological activities such as anti-glycation [ 193 ], anti-inflammatory [ 194 – 197 ], anti-microbial [ 198 , 199 ], anti-coagulant [ 200 – 204 ], anti-ageing [ 205 – 209 ] and anti-obesity [ 208 , 209 ] have also been recognized in seaweed polysaccharides.…”

Fish Waste: From Problem to Valuable Resource

“…PSC obtained from the skin of Lophius litulon was also tested. It has been shown to possess both antioxidant properties in vitro based on its ability to scavenge different free radicals, such as DPPH, ABTS·, HO·, and O 2 - and increase the content of superoxide dismutase (SOD) and catalase (CAT), and wound healing activity in mice model [ 87 ]. Collagen from red tilapia Oreochromis sp.…”

“… Skin regeneration, antibacterial 30 mg/kg BW [ 84 ] Collagen Skin Oreochromis sp. Wound healing Not specified [ 85 ] Collagen Scale Ctenopharyngodon idellus Wound healing Not specified [ 86 ] Collagen Skin Lophius litulon Antioxidant, wound healing 1–8 mg/mL4 g/day [ 87 ] Collagen Skin Oreochromis sp. Antioxidant 2–7 mg/mL [ 88 ] Collagen/chitosan Skin Not specified Antioxidant 12–100 μg/mL [ 89 ] Collagen/gelatin Skin Priacanthus hamrur Antioxidant, antibacterial DPPH and ABTS 1 mg/mL Antibacterial 200 μg [ …”

Using Collagen Peptides From the Skin of Monkfish (Lophius litulon) to Ameliorate Kidney Damage in High-Fat Diet Fed Mice by Regulating the Nrf2 Pathway and NLRP3 Signaling

“…The meat is usually made for human consumption, and the by-product skin is often treated as waste or used for making fish food ( 29 ). In our previous study, on which this study is based, we evaluated the physicochemical properties of extracts collected from the skin of monkfish ( Lophius litulon ) with pepsin (pepsin-solubilized collagen, PSC) and showed that PSC has good antioxidant and wound healing activity ( 30 ). In this study, PSC was hydrolyzed to obtain collagen peptides (pepsin-solubilized collagen peptide, PSCP) with higher antioxidant activity.…”