Abstract:Pepsin successfully catalyzed the synthesis of several peptide derivatives from N‐protected di‐ or tripeptides and amino acid or peptide esters or p‐nitroanilides in dimethylformamide‐water solutions at pH 4.6. An optimal substrates:pepsin ratio depended on the structure of starting peptides, especially their fit to the substrate binding sites of the enzyme. For hexapeptide Z‐Ala‐Ala‐Phe‐Leu‐Ala‐Ala‐OCH3 formation, an equilibrium yield was attained at 1:3. 105 enzyme‐substrates ratio that indicated high effici… Show more
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