Penundaan Pemeriksaan Differential Count Terhadap Gambaran Scattergram Hematology Analyzer Cell-Dyn Ruby

Abstract

Over the past few decades, diff count examinations have been performed using a hematology analyzer. It has been reported that delay in examination can change the morphology of leukocytes in peripheral blood smears. Cell-Dyn Ruby is a 5-part differential hematology analyzer with multiangle polarized scatter separation (MAPSS) technology that forms the WBC Differential scattergram and NEU-EOS scattergram. The purpose of this study was to obtain an overview of the Cell-Dyn Ruby scattergram diff count on the delayed examination material. The study used an Experimental Laboratory design, the examination used a Cell-Dyn Ruby hematology analyzer. 0 hours examination as a control, 6, 12 and 24 hours examination as treatment. A total of 30 respondents participated in this study and obtained a scattergram image after the delay in the form of an increase in the number of dots in black and pink, as well as a widening of the distribution of orange dots. Based on statistical tests, there were significant differences in the results of the examination (P <0.01) in all parameters of the leukocyte examination consisting of white blood cells (WBC), segmented neutrophils (SEG), band neutrophils (BAND), immature granulocytes (IG), monocytes (MONO), eosinophils (EO), basophils (BASO) and lymphocytes (LYM). Our results show that the examination delay can be detected by the Cell-Dyn Ruby scattergram especially the WBC Differential channel after a delay of 12 hours, and is noticeable after a delay of 24 hours. The suggestion of this research is the use of a scattergram for verification of the feasibility of examination materials and the need for further research to validate with blood smears and comparisons with other tools that have different technologies.

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