Pentraxin‐3 detected in human saliva shows limited correlation with biomarkers associated with systemic inflammation

Wetterö, Jonas; Jönsson, Frida; Löhneysen, Sarah von; Kristenson, Margareta; Garvin, Peter; Sjöwall, Christopher

doi:10.1111/apm.13136

Cited by 3 publications

(3 citation statements)

References 42 publications

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“…Second, levels of sCRP and other salivary biomarkers have been proven to vary during the day ( 32 , 51 – 53 ), with a peak at awakening and lower levels during daytime ( 51 ). Such diurnal variation might have influenced the reliability of sCRP levels measured in our study, since sampling took place at different times of the day.…”

Section: Discussionmentioning

confidence: 99%

Comparison of C-Reactive Protein in Dried Blood Spots and Saliva of Healthy Adolescents

et al. 2021

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Background/AimDetermining C-reactive protein (CRP) by non-invasive methods is of great interest for research addressing inflammation in young people. However, direct comparisons of such methods applied in children and adolescents are lacking so far. This study aimed to evaluate the association between CRP measured in dried blood spots (DBS CRP) and in saliva (sCRP), two less invasive alternatives to venipuncture, in 12- to 14-year-old adolescents. To evaluate the validity of both measurements in the context of biobehavioral studies, the potential of DBS CRP and sCRP to discriminate between defined BMI subgroups was assessed.Materials and MethodsCRP levels in DBS and saliva collected from 87 healthy adolescents (M = 13.25 years, SD = 0.30, 51.7% females) were determined using high sensitive CRP ELISA for serum and salivary CRP ELISA, respectively. Characteristics and correlation of both measurements were assessed for the total sample and for three subgroups classified by BMI percentile ranges (A: ≤ 25; B: 26–74; C: ≥ 75).ResultsIn the total sample, DBS CRP and sCRP were significantly associated (r = 0.59, p < 0.001). Splitting the sample into BMI-dependent subgroups revealed similarly strong associations of DBS CRP with sCRP for all three groups (A: r = 0.51; B: r = 0.61; C: r = 0.53). However, comparing the mean CRP values per BMI subgroup, one-way ANOVA reported significant differences for DBS CRP, but not for sCRP mean values.ConclusionsThe significant correlation of DBS CRP with sCRP was independent of the investigated BMI range groups, yet BMI-dependent distinction was only provided by DBS CRP mean values. Overall, our results suggest that DBS CRP is likely to reflect systemic inflammation more precisely. Salivary CRP can be alternatively determined in studies with adolescents when conditions require it, given the oral health status is assessed. Considering that DBS CRP and sCRP share only 35% of common variance, further studies should examine their specific validity.

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Section: Discussionmentioning

confidence: 99%

Comparison of C-Reactive Protein in Dried Blood Spots and Saliva of Healthy Adolescents

et al. 2021

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“…A DuoSet enzyme-linked immunosorbent assay (ELISA; detection limit 0.22 µg/L) kit was used to analyze PTX3 levels in plasma (R&D Systems, Minneapolis, MN, USA). Assays were performed according to the manufacturers’ instructions as previously described ( 29 , 30 ). Briefly, Costar (Corning, NY, USA) half‐area plates were coated with 2 µg/mL of mouse anti‐human PTX3 and incubated overnight.…”

Section: Methodsmentioning

confidence: 99%

Plasma C-Reactive Protein and Pentraxin-3 Reference Intervals During Normal Pregnancy

et al. 2021

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Although several biomarkers are available to monitor the acute phase response, the short pentraxin C-reactive protein (CRP) is dominating in clinical practice. The long pentraxin 3 (PTX3) is structurally and functionally related to CRP, but not liver-derived. In addition, increased levels of PTX3 have been linked to preeclampsia. Reference intervals are usually based on healthy blood donors. Several physiological and immunological alterations occur during normal pregnancy with subsequent potential effects on blood analytes. Hence, this study aims to determine pregnancy-specific reference intervals for CRP and PTX3. Longitudinal clinical data and blood plasma samples from the 1st, 2nd and 3rd trimester of 100 healthy, non-medicating, females aged 18–40 at the time-point of conception were available to us. High‐sensitivity CRP measurements were performed by turbidimetry and enzyme-linked immunosorbent assay (ELISA) was used to quantify PTX3. CRP and PTX3 levels followed each other during the first two trimesters and both increased during the third trimester. CRP showed a median of 4.12 mg/L in the third trimester, and were significantly higher compared to the first (median 2.39 mg/L, p<0.0001) and the second (median 2.44 mg/L, p=0.0006) trimesters. In the third trimester PTX3 levels reached a median of 7.70 µg/L, and were significantly higher compared to the first (median 3.33 µg/L, p<0.0001) and the second (median 3.70 µg/L, p<0.0001) trimesters. Plasma albumin was inversely correlated with CRP (rho=-0.27, p<0.0001), but not with PTX3. In conclusion, it is important to consider pregnancy-specific reference values as elevations of CRP and PTX3 during the later phase may occur in absence of infection.

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“…Smoking showed lower PTX3 levels. Gheorghe et al 2021 [55] GCF CRP and PTX3 correlated with levels of periodontal inflammation. It reduced with non-surgical periodontal therapy.…”

Section: Gcf and Salivamentioning

confidence: 96%

Role of Pentraxin-3 in Periodontal Inflammation - A Comprehensive Review

Peeran

Elhassan

Dawood

et al. 2021

JPRI

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Acute phase reactants like C-reactive protein (CRP), and pentraxin 3 (PTX3) are increased with inflammation and tissue injury. PTX3 is an acute phase protein and a member of the long pentraxin family. CRP is synthesized in the liver but PTX3 is generated locally at the inflammatory site. It is a fluid-phase pattern-recognition molecule that regulates antimicrobial immunity and inflammation by interfering with selectin-dependent neutrophil recruitment and regulating the complement cascade. Hence, PTX3 could be used as a potential biomarker to identify inflammatory response in both acute and chronic diseases. In this review, we discuss the role of PTX3 in periodontal inflammation.

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Pentraxin‐3 detected in human saliva shows limited correlation with biomarkers associated with systemic inflammation

Cited by 3 publications

References 42 publications

Comparison of C-Reactive Protein in Dried Blood Spots and Saliva of Healthy Adolescents

Comparison of C-Reactive Protein in Dried Blood Spots and Saliva of Healthy Adolescents

Plasma C-Reactive Protein and Pentraxin-3 Reference Intervals During Normal Pregnancy

Role of Pentraxin-3 in Periodontal Inflammation - A Comprehensive Review

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