Pentoxifylline decreases tumor necrosis factor and interleukin-1 during high tidal volume

Oliveira-Júnior, Itamar Souza; Pinheiro, Bruno Valle; Silva, Ismael Dale Cotrim Guerreiro da; Salomão, Reinaldo; Zollner, Ricardo de Lima; Beppu, Osvaldo Shigueomi

doi:10.1590/s0100-879x2003001000011

Cited by 26 publications

(22 citation statements)

References 34 publications

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“…The other portions of lungs were homogenized and mRNA for TNF-a, IL-10, eNOS, and b-actin (housekeeping gene) expression was performed by RT-PCR. 20 Total RNA was isolated from the lung of 5 animals per group and the RT-PCR was previously described by performed in the lungs according to the following groups: 4, 7, 14, 21, and 28 days. Total RNA was extracted using Trizol reagent according to manufacturer's protocol (Gibco-BRL, Gaithersburg, MD), and the RNA was ethanol precipitated and dissolved in diethyl pyrocarbonate-treated water and total RNA was quantified spectrophotometrically at 260 and 280 nm (Genesys 5, Spectronic Instruments, Rochester, NY), RNA concentration (1 µg/1 µL).…”

Section: Animalsmentioning

confidence: 99%

Pulmonary Disease in Hamsters Infected with Leptospira interrogans: Histopathologic Findings and Cytokine mRNA Expressions

Marinho

Oliveira-Júnior²,

Monteiro³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. Our aim was to evaluate the pulmonary changes induced by Leptospira interrogans infection in hamsters, and the gene expression of endogenous mediators in lung fragments during 28 days of observation. The animals were euthanized on days 4, 7, 14, 21, and 28 post-inoculation. Histopathologic lung analysis showed hemorrhage, pneumonia, alveolar congestion, and infiltrated cellular areas, with increasing severity until day 21 post-inoculation. Tumor necrosis factor (TNF)-a mRNA expression enhanced in first days with peak on day 4 and slightly decreased in the final phase. The interleukin (IL)-10 remained relatively constant throughout the period, with the exceptions of days 4 and 14. The endothelial nitric-oxide synthesis (eNOS) showed an increased expression on day 4, followed by an augment on days 7 and 14, and remaining constant up to day 28 post-infection. Our results demonstrate that inoculation of L. interrogans sorovar Icterohaemorrhagiae induced pulmonary lesions, including pulmonary hemorrhage, supporting that the lung is a target organ.

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Section: Animalsmentioning

confidence: 99%

Pulmonary Disease in Hamsters Infected with Leptospira interrogans: Histopathologic Findings and Cytokine mRNA Expressions

Marinho

Oliveira-Júnior²,

Monteiro³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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“…PTX is a phosphodiesterase inhibitor that has been shown to decrease TNF-α levels and to down-regulate neutrophil activation, probably by increasing intracellular cyclic AMP (27). We have previously shown that PTX protects rats from lung injury induced by ventilation with a high tidal volume (15).…”

Section: Discussionmentioning

confidence: 99%

“…We have shown that PTX protects lung dysfunction secondary to ventilatory support (ventilator-induced lung injury) by abolishing the local and systemic release of TNF-α and IL-1ß (15).…”

Section: Is Oliveira-junior Et Almentioning

confidence: 98%

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Effect of pentoxifylline on lung inflammation and gas exchange in a sepsis-induced acute lung injury model

Oliveira-Júnior

Brunialti

Koh

et al. 2006

Braz J Med Biol Res

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Experimental models of sepsis-induced pulmonary alterations are important for the study of pathogenesis and for potential intervention therapies. The objective of the present study was to characterize lung dysfunction (low PaO 2 and high PaCO 2 , and increased cellular infiltration, protein extravasation, and malondialdehyde (MDA) production assessed in bronchoalveolar lavage) in a sepsis model consisting of intraperitoneal (ip) injection of Escherichia coli and the protective effects of pentoxifylline (PTX). Male Wistar rats (weighing between 270 and 350 g) were injected ip with 10 7 or 10 9 CFU/100 g body weight or saline and samples were collected 2, 6, 12, and 24 h later (N = 5 each group). PaO 2 , PaCO 2 and pH were measured in blood, and cellular influx, protein extravasation and MDA concentration were measured in bronchoalveolar lavage. In a second set of experiments either PTX or saline was administered 1 h prior to E. coli ip injection (N = 5 each group) and the animals were observed for 6 h. Injection of 10 7 or 10 9 CFU/100 g body weight of E. coli induced acidosis, hypoxemia, and hypercapnia. An increased (P < 0.05) cell influx was observed in bronchoalveolar lavage, with a predominance of neutrophils. Total protein and MDA concentrations were also higher (P < 0.05) in the septic groups compared to control. A higher tumor necrosis factor-alpha (P < 0.05) concentration was also found in these animals. Changes in all parameters were more pronounced with the higher bacterial inoculum. PTX administered prior to sepsis reduced (P < 0.05) most functional alterations. These data show that an E. coli ip inoculum is a good model for the induction of lung dysfunction in sepsis, and suitable for studies of therapeutic interventions. Correspondence

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“…Artificial ventilation is one of the most important techniques in the ICU, besides being an important tool in the approach of the acute lung injury/acute respiratory distress syndrome (ALI/ARDS) of any nature or in other cases 7,8 . In many cases of respiratory diseases, the use of ventilatory support is required.…”

Section: Introductionmentioning

confidence: 99%

Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs

et al. 2011

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Purpose:To evaluate the effects of mechanical ventilation (MV) of high-oxygen concentration in pulmonary dysfunction in adult and elderly rats. Methods: Twenty-eight adult (A) and elderly (E), male rats were ventilated for 1 hour (G-AV1 and G-EV1) or for 3 hours (G-AV3 and G-EV3). A and E groups received a tidal volume of 7 mL/kg, a positive end-expiratory pressure of 5 cm H2O, respiratory rate of 70 cycles per minute, and an inspiratory fraction of oxygen of 1. We evaluated total protein content and malondialdehyde in bronchoalveolar lavages (BAL) and performed lung histomorphometrical analyses. Results: In G-EV1 animals, total protein in BAL was higher (33.0±1.9 µg/mL) compared with G-AV1 (23.0±2.0 µg/mL). Upon 180 minutes of MV, malondialdehyde levels increased in elderly (G-EV3) compared with adult (G-AV3) groups. Malondialdehyde and total proteins in BAL after 3 hours of MV were higher in elderly group than in adults. In G-EV3 group we observed alveolar septa dilatation and significative increase in neutrofiles number in relation to adult group at 60 and 180 minutes on MV. Conclusion: A higher fraction of inspired oxygen in short courses of mechanical ventilation ameliorates the parameters studied in elderly lungs.

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Pentoxifylline decreases tumor necrosis factor and interleukin-1 during high tidal volume

Cited by 26 publications

References 34 publications

Pulmonary Disease in Hamsters Infected with Leptospira interrogans: Histopathologic Findings and Cytokine mRNA Expressions

Pulmonary Disease in Hamsters Infected with Leptospira interrogans: Histopathologic Findings and Cytokine mRNA Expressions

Effect of pentoxifylline on lung inflammation and gas exchange in a sepsis-induced acute lung injury model

Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs

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