Pentoxifylline augments TRAIL/Apo2L mediated apoptosis in cutaneous T cell lymphoma (HuT-78 and MyLa) by modulating the expression of antiapoptotic proteins and death receptors

Gahlot, Satindra; Khan, Mohammad Aslam; Rishi, Loveena; Majumdar, S.

doi:10.1016/j.bcp.2010.08.018

Cited by 16 publications

(15 citation statements)

References 46 publications

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“…Both the cell lines were passaged for less than 3 months before use in this study. These cell lines were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS, GIBCO (16). Curcumin, N-acetyl-Lcysteine (NAC), rapamycin, 3-methyladenine (3-MA), wortmannin, propidium iodide (PI), 3,3 0 -dihexyloxacarbocyanine iodide (DiOC 6 ), anti-actin antibody, antimouse horseradish peroxidase antibody, and anti-rabbit horseradish peroxidase antibody were purchased from Sigma.…”

Section: Cell Culture and Reagentsmentioning

confidence: 99%

“…Cell viability was determined by PI exclusion assay (16). Briefly, 2 Â 10 5 cells (HuT-78 or MyLa) were treated with different concentration of curcumin (0-25 mmol/L) for 24 hours (18).…”

Section: Pi Exclusion Assay For Cell Viabilitymentioning

confidence: 99%

“…To measure the DNA strand breaks during apoptosis, TUNEL assay was conducted using APO-BRDU Kit (BD PharMingen) according to manufacturer's protocol as described by Gahlot and colleagues (16). Stained cells were analyzed with flow cytometry.…”

Section: Terminal Deoxynucleotidyl Transferase-mediated Dutp Nick Endmentioning

confidence: 99%

“…Annexin V staining kit (BD PharMingen) was used as described previously (16). Stained cells were analyzed with a FACSCalibur (Becton Dickinson).…”

Section: Annexin V Stainingmentioning

confidence: 99%

“…For Western blot analysis, cell lysates were prepared after treatment as described previously (16). Protein extracts were resolved on 10% to 12% SDS-PAGE and analyzed by Western blotting using specific antibodies against caspase-2, Bid, cytochrome c, PARP, c-FLIP, BclxL, cIAP-1, cIAP-2, XIAP, Hsp90, Hsp70, IKK-a, IKK-b, beclin-1, and LC3.…”

Section: Western Blot Analysismentioning

confidence: 99%

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Oxidative Stress Induced by Curcumin Promotes the Death of Cutaneous T-cell Lymphoma (HuT-78) by Disrupting the Function of Several Molecular Targets

Khan

Gahlot

Majumdar

2012

Molecular Cancer Therapeutics

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Curcumin is known to exert its anticancer effect either by scavenging or by generating reactive oxygen species (ROS). In this study, we report that curcumin-mediated rapid generation of ROS induces apoptosis by modulating different cell survival and cell death pathways in HuT-78 cells. Curcumin induces the activation of caspase-8, -2, and -9, alteration of mitochondrial membrane potential, release of cytochrome c, and activation of caspase-3 and concomitant PARP cleavage, but the addition of caspase inhibitors only partially blocked the curcumin-mediated apoptosis. Curcumin also downregulates the expression of antiapoptotic proteins c-FLIP, Bcl-xL, cellular inhibitor of apoptosis protein, and X-linked IAP in a ROS-dependent manner. Curcumin disrupts the integrity of IKK and beclin-1 by degrading Hsp90. Degradation of IKK leads to the inhibition of constitutive NF-kB. Degradation of beclin-1 by curcumin leads to the accumulation of autophagy-specific marker, microtubule-associated protein-I light chain 3 (LC3), LC3-I. Our findings indicate that HuT-78 cells are vulnerable to oxidative stress induced by curcumin and as a result eventually undergo cell death. Mol Cancer Ther; 11(9); 1873-83. Ó2012 AACR.

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Section: Cell Culture and Reagentsmentioning

confidence: 99%

“…Cell viability was determined by PI exclusion assay (16). Briefly, 2 Â 10 5 cells (HuT-78 or MyLa) were treated with different concentration of curcumin (0-25 mmol/L) for 24 hours (18).…”

Section: Pi Exclusion Assay For Cell Viabilitymentioning

confidence: 99%

Section: Terminal Deoxynucleotidyl Transferase-mediated Dutp Nick Endmentioning

confidence: 99%

“…Annexin V staining kit (BD PharMingen) was used as described previously (16). Stained cells were analyzed with a FACSCalibur (Becton Dickinson).…”

Section: Annexin V Stainingmentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

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Oxidative Stress Induced by Curcumin Promotes the Death of Cutaneous T-cell Lymphoma (HuT-78) by Disrupting the Function of Several Molecular Targets

Khan

Gahlot

Majumdar

2012

Molecular Cancer Therapeutics

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Cristazine, a novel dioxopiperazine alkaloid, induces apoptosis via the death receptor pathway in A431 cells

Patil

Jung

et al. 2019

Drug Development Research

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The fungus Chaetomium sp. is a causative agent of infections in humans and is ubiquitous in the natural environment. The secondary metabolites of this genus exhibit many biological activities, including antifungal activity and toxicity in mitochondria. In this study, we isolated cristazine from the fungus C. cristatum, which has the potential to inhibit the growth of human epidermoid carcinoma (A431) cells in a dose-and time-dependent manner. Its inhibitory activity was examined using a cell viability assay and cell death was elucidated by western blot analysis. Cristazine triggered apoptotic cell death via the Type I death receptor pathway including the activation of caspases and other target proteins. However, cristazine did not have any effect on mitochondrial apoptotic proteins such as Bid, cytochrome c, and apoptosis-inducing factor. Cristazine inhibited the cell cycle progression by arresting the G 1 /S phase and up-regulating the inhibitory proteins of cyclin-dependent kinases. Thus, cristazine has great potential for inducing apoptosis in A431 cells via both cell cycle arrest and the inhibition of cell growth. K E Y W O R D S cell cycle arrest, Chaetomium cristatum, cristazine, death receptor apoptosis, human epidermoid carcinoma

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Pentoxifylline during steroid window phase at induction to remission increases apoptosis in childhood with acute lymphoblastic leukemia

et al. 2015

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Pentoxifylline augments TRAIL/Apo2L mediated apoptosis in cutaneous T cell lymphoma (HuT-78 and MyLa) by modulating the expression of antiapoptotic proteins and death receptors

Cited by 16 publications

References 46 publications

Oxidative Stress Induced by Curcumin Promotes the Death of Cutaneous T-cell Lymphoma (HuT-78) by Disrupting the Function of Several Molecular Targets

Oxidative Stress Induced by Curcumin Promotes the Death of Cutaneous T-cell Lymphoma (HuT-78) by Disrupting the Function of Several Molecular Targets

Cristazine, a novel dioxopiperazine alkaloid, induces apoptosis via the death receptor pathway in A431 cells

Pentoxifylline during steroid window phase at induction to remission increases apoptosis in childhood with acute lymphoblastic leukemia

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